The simultaneous separation and determination of six flavonoids and troxerutin in rat urine and chicken plasma by reversed-phase high-performance liquid chromatography with ultraviolet–visible detection

Sensitive determination of kaempferol in rat plasma by high-performance liquid chromatography with chemiluminescence detection and application to a pharmacokinetic study

“…The linear range and detection limit was compared with those obtained by HPLC with UV, FD, ECD, and MS detection in the literatures. Table 1 indicates that the proposed method has wider linear range and lower detection limit than that of the method using UV [18,21], FD [14], and ECD [17], and is almost as sensitive as that of HPLC-MS-MS method [19]. Therefore, the present HPLC-CL method does offer an alternative, sensitive and simple approach to the detection of kaempferol.…”

“…Several mobile phases have been reported for the separation of flavonols on RP-C18 column, such as methanol-tetrafluoracetic acid-water [16], methanol-acetic acid-water [21], and acetonitrile-acetic acid-water [24]. In this work, the mobile phase of methanol-phosphoric acid-water was found to be suitable for the separation of flavonols and compatible with the cerium(IV)-rhodamine 6G CL system in sulfuric acid medium.…”

“…Up to now, analysis of flavonoids has been accomplished by thin-layer chromatography (TLC) [10], gas chromatography (GC) [11,12], capillary electrophoresis (CE) [13,14], micellar electrokinetic chromatography (MEKC) [15], and high-performance liquid chromatography (HPLC) [16][17][18][19][20][21][22][23][24][25]. For the separation and determination of kaempferol in biological samples, high-performance liquid chromatography (HPLC) has been shown to be suitable, in which UV [24], electrochemical [19], fluorescent [17] and mass [22] detections were used.…”

Simultaneous determination of nine flavonoids in beagle dog by HPLC with DAD and application ofGinkgo bilobaextracts on the pharmacokinetic

“…Several previous papers [10][11][12][13] have described high-performance liquid chromatography (HPLC) methods for the simultaneous determination of one or several flavonoids in plasma, but in these researches, administrated drug was single compound or mixture of several flavonoids, so these methods are not suitable for analysis of flavonoids in beagle dog plasma after being administrated GBE. Some scholars have published analysis method of Ginkgo flavonoids in dog or rabbit plasma by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) [14] or HPLC [15][16], but they only developed the simultaneous determination of quercetin, kaempferol, and isorhamnetin using acid hydrolysis or calculated total peak area of GBE in plasma.…”

Simultaneous determination of five flavonoids inSaussurea mongolicaFranch by high-performance liquid chromatography

“…Because of the bioenhancing effects of flavonoids on drugs and nutriments, the determination of flavonoids in Saussurea species will play an essential part in research on the efficacy, safety, and therapeutic reproducibility of this medicinal plant. Several assays have been described for the determination of flavonoids based on gas chromatography (GC) [13], thin layer chromatography (TLC) [14], high-performance liquid chromatography with ultra-violet detection (HPLC-UV) [15,16], HPLC with evaporative light scattering detection (HPLC-ELSD) [17], capillary electrophoresis (CE) [18], gas chromatography with mass spectrometry (GC-MS) [19], HPLC with mass spectrometry (HPLC-MS) [20,21], and with nuclear magnetic resonance spectroscopy (HPLC-NMR) [22].…”