The small GTPase NtRHO1 negatively regulates tobacco defense response to tobacco mosaic virus by interacting with NtWRKY50

Abstract

Small GTPases play critical roles in the regulation of plant growth and development. However, the mechanism of small GTPases in plant response to virus infection remains largely unknown. Here, a Rho-type GTPase NtRHO1 was identified as one of up-regulated genes after tobacco mosaic virus (TMV) infection. Subcellular localization of NtRHO1 showed that it was localized in the cytoplasm, plasma membrane as well as nucleus. Transient overexpression of NtRHO1 in Nicotiana benthamiana plants accelerated virus reproduction and led to more reactive oxygen species production. By contrast, silencing of NtRHO1 reduced the sensitivity of N. benthamiana plants to TMV-GFP. Further explorations showed that there existed a direct interaction between NtRHO1 and NtWRKY50, a positive regulator of N. benthamiana plants response to virus infection. Yeast one-hybrid and electrophoretic mobility shift assays showed that this regulation was related to NtWRKY50’s binding capacity to the WK-box of PR1 promoter, which was weakened by the interaction between NtRHO1 and NtWRKY50. Thus, the role of a novel small GTPase NtRHO1 in the plant-pathogen interaction was explored and its mechanism was proposed.

Comprehensive Analysis of Subcellular Localization, Immune Function and Role in Bacterial wilt Disease Resistance of Solanum lycopersicum Linn. ROP Family Small GTPases

“…Similarly, CA-HvRAC1 triggered peroxide burst but also supported the penetration of biotrophic Bgh [ 44 ]. Downstream interactors of Rac/Rop small GTPases played critical roles in the defense system by regulating multiple responses against pathogens, such as the expression of pathogenesis-related genes and the biosynthesis of lignin [ 21 , 23 , 24 , 35 ]. Therefore, the variable downstream molecules targeted by SlRops might lead to different outputs when infected by R. solanacearum , which causes tomato plants to display varying degrees of insusceptibility.…”

“…Moreover, TaRop10, the group II ROP from wheat, showed negative regulation of wheat immunity against stripe rust [ 34 ]. In addition, tobacco NtRHO1 plays a negative role in defense response to TMV [ 35 ]. In conclusion, Rho-related small GTPases perform a dual function via a variety of signal transductions during pathogen infections.…”

Alpha‐momorcharin preserves catalase activity to inhibit viral infection by disrupting the 2b–CAT interaction in Solanum lycopersicum

“…Amplifications of the Actin gene in tomato and EF1α in tobacco served as internal references. Finally, relative expression levels of genes were calculated using the comparative 2 −∆∆ C t method (Han et al, 2022 ). All primers used in this study are shown in Table S1 .…”

ZmRop1 participates in maize defense response to the damage of Spodoptera frugiperda larvae through mediating ROS and soluble phenol production

“…The content of reactive oxygen in maize plants overexpressing or silencing ZmRop1 was determined by nitroblue tetrazolium (NBT) staining (Han et al, 2022 ). The same parts of maize plants after different treatments were cut in Petri dishes, stained with NBT solution and exposed to light for 1 h, then decolorized with 90% ethanol solution in a water bath at 65°C for 10–15 min, and the leaves were observed.…”