The spectrum of enzymes involved in activation of 2-aminoanthracene varies with the metabolic system applied

Veres, Zsuzsa; Török, György; Tóth, E.; Vereczkey, L.; Jemnitz, Katalin

doi:10.1016/j.mrgentox.2005.05.009

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…2AA is a pre-carcinogen; its reactive electrophilic metabolites bind with DNA [51]. All of the EXTs exhibited high antigenotoxic potential against 2AA-induced genotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Antigenotoxic, Anti-photogenotoxic, and Antioxidant Properties of Polyscias filicifolia Shoots Cultivated In Vitro

et al. 2020

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Traditional medicinal plants are an important source of active compounds with potential antimutagenic activity. Polyscias filicifolia Bailey (Araliaceae) is a South Asian traditional herb used as an adaptogenic and cardiac drug. Extracts of P. filicifolia contain a wide range of biologically active compounds like phenolic acids and triterpenoid saponins. In the present study. antigenotoxic potential of three naturally occurring phenolic acids and extracts of P. filicifolia growing in vitro with the addition of elicitors was evaluated against direct (4-nitroquinoline-N-oxide (4NQO) and mitomycin C (MMC)) and indirect mutagens (2-aminoanthracene (2AA)). The evaluation was made using a bacterial umu-test. Moreover, the ability to prevent photogenotoxicity induced by chlorpromazine (CPZ) under UVA irradiation was measured. The phytochemical profiling of examined extracts revealed the presence of numerous compounds with the prevelance of chlorogenic, caffeic, and ferulic acid derivatives; however, saponin fractions were also determined. The antioxidant potential of extracts strictly correlated with their composition. The tested extracts exhibited high antigenotoxic activity if the assay was performed with 2AA and metabolic activation. Moreover, the extracts slightly decreased the MMC-induced genotoxicity. However, an increase of the genotoxic effect was observed in the assay performed with 4NQO. In addition, photo-antigenotoxic activity was observed. In our study, phenolic acids exhibited lower activity than the extracts.

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“…2AA is a pre-carcinogen; its reactive electrophilic metabolites bind with DNA [51]. All of the EXTs exhibited high antigenotoxic potential against 2AA-induced genotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Antigenotoxic, Anti-photogenotoxic, and Antioxidant Properties of Polyscias filicifolia Shoots Cultivated In Vitro

et al. 2020

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“…The supernatants were stored at −80 • C until use. The protein content was determined by the method of Lowry using bovine serum albumin as the standard [22]. The in vitro metabolic activation system consisted of rat liver P450 enzymes, which were contained in S9, and an energy-producing system necessary for their function (NADP and glucose-6-phosphate) [8,10].…”

Section: Metabolic Activation Systemmentioning

confidence: 99%

In vitroevaluation of the genotoxicity of a family of novel MeO-PEG-poly(D,L-lactic-co-glycolic acid)-PEG-OMe triblock copolymer and PLGA nanoparticles

Yang

Zhang

et al. 2009

Nanotechnology

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Despite the booming development of nanoparticle materials for pharmaceutical applications, studies on their genotoxicity are few. In our previous efforts to develop an intravenous nanoparticle material, a family of novel monomethoxy(polyethylene glycol)-poly(D,L-lactic-co-glycolic acid)-monomethoxy (PELGE) polymers was synthesized. The cytotoxicity and genotoxicity of nine kinds of selected blank PELGE and PLGA (poly(D,L-lactic and glycolic acid)) nanoparticles were evaluated using methyl thiazolyl tetrazolium (MTT), micronucleus (MN) and sister chromatid exchange (SCE) assays with or without the addition of a metabolic activation system (S9 mix), using Chinese hamster ovary (CHO) cells. The cytotoxicity of nanoparticles exhibited a dose-dependent response, with a concentration of 5 mg ml(-1) being the turning point. The frequencies of MN observed in samples treated with various nanoparticles were not statistically different from those seen in the negative controls in the presence or absence of the S9 mix. Also, no cell cycle delay was observed. The numbers of SCE per cell observed in samples treated with five kinds of PELGE nanoparticles were significantly greater than those found in the negative controls with or without the S9 mix. The discrepancies found in the two assays suggest that the five kinds of nanoparticles may produce only a weakly clastogenic response.

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Oxidative stress-mediated genotoxicity of wastewaters collected from two different stations in northern India

Tabrez¹,

Ahmad

2011

Mutation Research/Genetic Toxicology and Environmental Mutagene

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The spectrum of enzymes involved in activation of 2-aminoanthracene varies with the metabolic system applied

Cited by 6 publications

References 28 publications

Antigenotoxic, Anti-photogenotoxic, and Antioxidant Properties of Polyscias filicifolia Shoots Cultivated In Vitro

Antigenotoxic, Anti-photogenotoxic, and Antioxidant Properties of Polyscias filicifolia Shoots Cultivated In Vitro

In vitroevaluation of the genotoxicity of a family of novel MeO-PEG-poly(D,L-lactic-co-glycolic acid)-PEG-OMe triblock copolymer and PLGA nanoparticles

Oxidative stress-mediated genotoxicity of wastewaters collected from two different stations in northern India

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