1997
DOI: 10.1016/s0092-8674(00)80213-5
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The SREBP Pathway: Regulation of Cholesterol Metabolism by Proteolysis of a Membrane-Bound Transcription Factor

Abstract: erstein and Fagan, 1966). The latter is converted to isopentenyl pyrophosphate, which is polymerized and Southwestern Medical Center Dallas, Texas 75235 modified to form the 27 carbons of cholesterol. Cholesterol accumulation lowers the activity of HMG CoA reductase and several other enzymes in the cholesterol biosynthetic pathway, thereby limiting the production Animal cells must regulate their biosynthetic pathways of cholesterol (reviewed in Goldstein and Brown, 1990). so as to produce the required amounts … Show more

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Cited by 3,407 publications
(2,893 citation statements)
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“…The SREBP-1c transcription factor acts as a key regulator of fatty acid synthesis by regulating multiple enzymes required for fatty acid biosynthesis (Brown and Goldstein, 1997). The mRNA level of SREBP-1c was not significantly influenced by lipid type or C. butyricum addition.…”
Section: Discussionmentioning
confidence: 93%
“…The SREBP-1c transcription factor acts as a key regulator of fatty acid synthesis by regulating multiple enzymes required for fatty acid biosynthesis (Brown and Goldstein, 1997). The mRNA level of SREBP-1c was not significantly influenced by lipid type or C. butyricum addition.…”
Section: Discussionmentioning
confidence: 93%
“…Rabbit polyclonal anti-insulin receptor antibody was from Santa Cruz Biotechnology (Mile Elm, UK). Mouse monoclonal anti-sterol regulatory element binding protein-1c precursor (SREBP) [32] was from F. Foufelle (Unit 465 INSERM, Paris, France). Rabbit polyclonal anti-14-3-3-β antibody was raised against a peptide mapping at the amino terminus of the human protein (Autogen Bioclear UK, Mile Elm, UK).…”
Section: Methodsmentioning
confidence: 99%
“…Detection of Kir6.2 and SUR in subcellular fractions of MIN6 cells Equal volumes from the gradient fractions were separated on 9% (w/v) polyacrylamide gels then blotted onto Immobilon-P transfer membrane (Millipore, Watford, UK) and probed with organelle-specific antibodies against mGPDH (mitochondria), phogrin (large dense-core insulin-containing vesicle [LDCV] membranes) [30,35], TGN38 (Golgi) [31], insulin receptor (IR, plasma membrane); LAMP-1 (lysosomes) [36]; SREBP precursor (endoplasmic reticulum [ER]) [32]; 14-3-3-β (cytosol) [37]. The protein and insulin contents in each fraction were determined using a Pierce BCA Protein Assay Kit (Rockford, IL, USA) and a Mercodia Ultrasensitive Mouse Insulin ELISA Kit (Uppsala, Sweden), respectively.…”
Section: Methodsmentioning
confidence: 99%
“…SREBPs are transcription factors of the basic-helix-loophelix-leucine zipper (bHLH-Zip) family and exist three isoforms, SREBP-1a, SREBP-1c and SREBP-2 (Brown and Goldstein, 1997). SREBPs play a central role in cellular lipid homeostasis and regulate many genes encoding enzymes involved in cholesterol homeostasis and fatty acid biosynthesis (Brown and Goldstein, 1999).…”
Section: Srebpsmentioning
confidence: 99%