The Stability of Penicillin G Sodium in Aqueous Solution

Macek, Thomas J.; Hanus, Edward J.; Feller, Beate A.

doi:10.1002/jps.3030370809

Cited by 8 publications

(9 citation statements)

References 8 publications

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“…Thomas (46) claimed that phosphate, citrate, and acetate buffers all stabilized penicillin. On the other hand, Macek, et al (47), presented evidence which showed that the stabilizing effect of phosphates and citrates depends on their buffering action and not on any specific ion effect. Other workers (48,49) have shown that increasing concentrations of penicillin require increasing concentrations of phosphates to maintain optimum stability.…”

Section: Stability Of Penicillin I N Dosage Formsmentioning

confidence: 99%

Pharmaceutics of Penicillin

Schwartz

Buckwalter

1962

Journal of Pharmaceutical Sciences

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Section: Stability Of Penicillin I N Dosage Formsmentioning

confidence: 99%

Pharmaceutics of Penicillin

Schwartz

Buckwalter

1962

Journal of Pharmaceutical Sciences

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“…The p K a of the acid group in the Penicillin G molecule is around 2.7, so its average affinity for hydrophobic adsorbents increases when the pH decreases since there is a greater quantity of neutral species present in the solution. However, the stability of this penicillin is much higher at neutral pH than at low pH. − Therefore, the efficiency of the adsorption process is expected to be a trade-off between the yield and the integrity of the antibiotic.…”

Section: Introductionmentioning

confidence: 99%

Hydrophobic Adsorption Followed by Desorption with Ethanol–Water for Recovery of Penicillin G from Fermentation Broth

et al. 2020

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The hydrophobic adsorption is an alternative to traditional organic solvent extraction for the recovery and purification of Penicillin G (PenG). However, there is a lack of information concerning the effect of process variables and technical feasibility while balancing product degradation. After assessing the integrity of PenG under different conditions, Amberlite XAD-4 was selected from among three different adsorbents. During the batch process using only 0.05 g XAD-4 /mL medium , the adsorption yield increased from 36% at pH 6 to 44% at pH 4. More than 90% of the antibiotic was captured from the fermentation broth using 0.083 g XAD-4 /mL medium in a 45 min batch performed at pH 4 and 4 °C. Moreover, there was no PenG degradation. The desorption conditions were evaluated, and 95% of the antibiotic could be recovered in only one batch using water−ethanol, which is an unexplored PenG desorption process. The results showed selective adsorption, indicating that the process can also be useful for purification purposes. Hydrophobic adsorption with ethanol desorption is efficient, scalable, and green and could be used in place of traditional methods or in extractive fermentation.

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“…Snails were placed in fresh tanks every 24 h to maintain a relatively consistent antibiotic concentration as the stability of these antibiotics begins to decrease after 48 h at 37 °C (Sigma manufacturer’s instructions). Although the stabilities of these antibiotics differ in aqueous solution, all four have been shown to maintain efficacy aver a 24 h period at temperatures below 30 °C ( Benedict, Schmidt & Coghill, 1946 ; Cote et al, 2010 ; Fujiwara, Kawashima & Ohhashi, 1982 ; Macek, Hanus & Feller, 1948 ; Oswald & Nielsen, 1947 ; Pang, Guan & Cheng, 1984 ; Schwartz & Hayton, 1972 ). Each time snails were moved they were given fresh food (lettuce).…”

Section: Methodsmentioning

confidence: 99%

The behavioral effects of antibiotic treatment on the snailBiomphalaria glabrata

Allan¹,

Blouin²

2017

PeerJ

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Schistosomiasis is a detrimental neglected tropical disease that is transmitted by Planorbid snails. Understanding the transmission and control of this disease requires an extensive understanding of these intermediate hosts, which is only achieved by the effective rearing and study of species such as Biomphalaria glabrata. This species is the intermediate host for Schistosoma mansoni in the New World, and is also the main model for studying schistosomes in mollusks. Antibiotics are used routinely in B. glabrata tissue culture, and occasionally on live snails. Here we show that standard doses of three common antibiotics (penicillin, streptomycin and gentamicin) drastically diminish the activity of healthy B. glabrata, but that treated snails recover rapidly when placed in fresh water. Ampicillin treated snails did not show altered activity. We suggest that researchers keep these apparent toxicities in mind if a need for antibiotic treatment of live Planorbid snails arises.

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The Stability of Penicillin G Sodium in Aqueous Solution

Cited by 8 publications

References 8 publications

Pharmaceutics of Penicillin

Pharmaceutics of Penicillin

Hydrophobic Adsorption Followed by Desorption with Ethanol–Water for Recovery of Penicillin G from Fermentation Broth

The behavioral effects of antibiotic treatment on the snailBiomphalaria glabrata

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