The Sublethal Effects of β-Ecdysterone, a Highly Active Compound from Achyranthes bidentata Blume, on Grape Phylloxera, Daktulosphaira vitifoliae Fitch

Liu, Yongqiang; Yang, Fuqian; Pu, Hongtie; Su, Junhong; Liu, Zongjiang; Dhiloo, Khalid Hussain; Wang, Zhongyue

doi:10.1371/journal.pone.0165860

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…In some cases, however, the effect is the opposite, a phenomenon known as the replanting benefit 5 – 7 . A typical example of the latter phenomenon is that of the perennial herbaceous species Achyranthes bidentata (Amaranthaceae) 6 , 7 , a species cultivated for the medicinal properties thought to be present in the extracts of its dried roots, an ingredient widely used in traditional Chinese medicine 8 , 9 . The factors responsible for this replanting benefit are generally assumed to be associated with either unknown component(s) of its root exudate, changes in the nutritional environment and/or the effect of the first crop on the rhizosphere microflora 5 , 7 .…”

Section: Introductionmentioning

confidence: 99%

Integrated miRNA-mRNA analysis reveals the roles of miRNAs in the replanting benefit of Achyranthes bidentata roots

Yang

Yan

et al. 2021

Sci Rep

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The yield and quality of the medicinal plant Achyranthes bidentata can be increased when it is replanted into a field cultivated previously with the same crop, however, fundamental aspects of its biology (so-called “replanting benefit”) still remain to be elucidated. miRNAs are sRNA molecules involved in the post-transcriptional regulation of gene expression in plant biological processes. Here, 267 conserved and 36 novel miRNAs were identified in A. bidentata roots. We compared the miRNA content of the roots (R1) from first-year planting with that of the roots (R2) of second-year replanting, and screened 21 differentially expressed (DE) miRNAs. Based on in silico functional analysis, integrated miRNA-mRNA datasets allowed the identification of 10 miRNA-target family modules, which might participate in the benefit. The expression profiles of the miRNA-target modules were potentially correlated with the presence of the replanting benefit. The indication was that the miRNA-responsive continuous monoculture could reprogram miRNA-mRNA expression patterns, which possibly promote the root growth and development, enhance its transport activity and strengthen its tolerance to various stresses, thereby improving A. bidentata productivity as observed in the replanting benefit. Our study provides basic data for further research on the molecular mechanisms of the benefit in A. bidentata.

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Section: Introductionmentioning

confidence: 99%

Integrated miRNA-mRNA analysis reveals the roles of miRNAs in the replanting benefit of Achyranthes bidentata roots

Yang

Yan

et al. 2021

Sci Rep

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“…et al, 2016 ). As a result of AB has good medicinal value and pharmacological activity ( Vetrichelvan and Jagadeesan, 2002 ; Shen et al, 2011 ; Liu et al, 2016 ) for invigorating livers and kidneys ( Mitaine-Offer et al, 2001a , b ; Li et al, 2005 ; Liu et al, 2016 ; Yang et al, 2017 ), and sulfur-fumigation has been widely employed to protect AB from insects and molds, AB has become excessively sulfur-fumigated in China. And sulfur-fumigation could cause significant alteration of active constituents in AB.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of Characteristic Sulfur-Fumigated Markers and Chemical Transformation Mechanism for Quality Control of Achyranthes bidentate Blume Using Metabolome and Sulfur Dioxide Residue Analysis

et al. 2018

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Achyranthes bidentata Blume (AB) is a health food and a sulfur-free herbal medicine that is one of the most heavily sulfur-fumigated herbs in the marketplace. In this work, a comprehensive approach using ultra-performance liquid chromatography coupled with quadrupole time-of-flight-MS (UPLC-Q-TOF-MS) and multivariate statistical analysis was developed to identify characteristic sulfur-fumigation markers, elucidate chemical transformation mechanisms and characterize the degree of sulfur-fumigation of AB. Non-fumigated and sulfur-fumigated AB samples were compared by UPLC-Q-TOF-MS/MS analysis. Three triterpene saponins (Betavulgarosides II–IV) and two amides (Feruloyl-4-O-methyldopamine and Moupinamide) were identified as characteristic markers, which were positively correlated with two active AB components, namely oleanic acid and ferulic acid, respectively. Moreover, the extent of the sulfur-fumigation under different weight ratios of sulfur to herbal materials (1:20, 1:40, and 1:80) was analyzed based on chemical transformations and sulfur dioxide residues. Further verification showed that the ratio of 1:40 within 1 h was reasonable and efficient for herb quality preservation and assurance. This study provides a reliable sulfur-fumigation protocol for the quality control of AB and other herbs.

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The root transcriptome of Achyranthes bidentata and the identification of the genes involved in the replanting benefit

Yang

Yan

et al. 2018

Plant Cell Rep

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The transcriptome profiling in replanting roots revealed that expression pattern changes of key genes promoted important metabolism pathways, antioxidant and pathogen defense systems, adjusted phytohormone signaling and inhibited lignin biosynthesis. The yield of the medicinal plant Achyranthes bidentata could be significantly increased when replanted into a field cultivated previously for the same crop, but the biological basis of this so-called "replanting benefit" is unknown. Here, the RNA-seq technique was used to identify candidate genes responsible for the benefit. The analysis of RNA-seq libraries prepared from mRNA extracted from the roots of first year planting (normal growth, NG) and second year replanting (consecutive monoculture, CM) yielded about 40.22 GB sequencing data. After de novo assembly, 87,256 unigenes were generated with an average length of 1060 bp. Among these unigenes, 55,604 were annotated with public databases, and 52,346 encoding sequences and 2881 transcription factors were identified. A contrast between the NG and CM libraries resulted in a set of 3899 differentially transcribed genes (DTGs). The DTGs related to the replanting benefit and their expression profiles were further analyzed by bioinformatics and qRT-PCR approaches. The major differences between the NG and CM transcriptomes included genes encoding products involved in glycolysis/gluconeogenesis, glutathione metabolism and antioxidant defense, in aspects of the plant/pathogen interaction, phytohormone signaling and phenylpropanoid biosynthesis. The indication was that replanting material enjoyed a stronger level of defense systems, a balance regulation of hormone signals and a suppression of lignin formation, thereby promoting root growth and development. The study provides considerable significant insights for a better understanding of the molecular mechanism of the replanting benefit and suggests their possible application in developing methods to reinforce the effects in medicinal plants.

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The Sublethal Effects of β-Ecdysterone, a Highly Active Compound from Achyranthes bidentata Blume, on Grape Phylloxera, Daktulosphaira vitifoliae Fitch

Cited by 4 publications

References 36 publications

Integrated miRNA-mRNA analysis reveals the roles of miRNAs in the replanting benefit of Achyranthes bidentata roots

Integrated miRNA-mRNA analysis reveals the roles of miRNAs in the replanting benefit of Achyranthes bidentata roots

Elucidation of Characteristic Sulfur-Fumigated Markers and Chemical Transformation Mechanism for Quality Control of Achyranthes bidentate Blume Using Metabolome and Sulfur Dioxide Residue Analysis

The root transcriptome of Achyranthes bidentata and the identification of the genes involved in the replanting benefit

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