The subunits of the parasporal crystal of Bacillus thuringiensis: Size, linkage and toxicity

Huber, Hans E.; Lüthy, Peter; Ebersold, Hans-Rudolf; Cordier, J.-L.

doi:10.1007/bf00417171

Cited by 87 publications

(30 citation statements)

References 23 publications

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“…2B), were observed for both strains. Huber (24), reported that the majority of the crystals from B. thuringiensis, toxic to insects of the Lepidoptera order, are composed of proteins of approximately 130 kDa, which corresponds to the size of deltaendotoxins from the Cry1 class. The 70-kDa polypeptide may corresponds to delta-endotoxins from the Cry2 class, which have a molecular mass 71 kDa.…”

Section: Scanning Electronic Microscopy and Protein Profilementioning

confidence: 99%

A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

Gitahy¹,

De-Souza²,

Monnerat³

et al. 2007

Braz. J. Microbiol.

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The control of the major sugarcane pest, Diatraea saccharalis, is limited by the stem location of the caterpillar. As part of a long-term project towards the development of an alternative and efficient delivery system of Cry proteins to control the sugarcane borer, the current work describes the selection and characterization of a Brazilian B. thuringiensis strain with prominent activity towards D. saccharalis. Strain S76 was eleven-fold more active than the HD-1 Lepidoptera-standard strain, as estimated by the LC50 of 13.06 µg/L and 143.88 µg/ L, respectively. We observed bipiramidal and cuboidal crystals similar to those found in other B. thuringiensis strains with entomopathogenic activity against Lepidoptera and Diptera. In addition, smaller and spherical crystalline inclusions were also observed. The plasmid profile of strain S76 is similar to that of HD-1. PCR amplifications of S76 DNA using cry specific primers confirmed the presence of cry1Aa, cry1Ab, cry1Ac, cry2Aa1, and cry2Ab2, but not cry1Ad, cry2Ac and cry9 type genes. No differences that could explain the superior activity of S76 when compared to HD-1, the Lepidoptera standard strain, were observed. Nevertheless, its higher entomopathogenic activity has pointed this strain S76 as a potential source of cry genes to control sugarcane borer, an important pest that affects sugarcane, a crop that occupies a planted area of about 6 million ha in Brazil.

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Section: Scanning Electronic Microscopy and Protein Profilementioning

confidence: 99%

A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

Gitahy¹,

De-Souza²,

Monnerat³

et al. 2007

Braz. J. Microbiol.

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“…It was a monomer, while the crystal protein in the intact crystal or in the solution was suggested to be a dimer. 24 ) S. aureus V8 protease did not react with fragment T and (Xchymotrypsin took only small protions off, giving polypeptides of mol. wt.…”

Section: Discussionmentioning

confidence: 99%

A Toxic Fragment from the Entomocidal Crystal Protein ofBacillus thuringiensis

Nagamatsu

Itai

Hatanaka

et al. 1984

Agricultural and Biological Chemistry

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“…Additionally, it is necessary to prove the covalent attachment of sugars, not merely their presence. Huber et al (13) detected <0.1% neutral sugars in extensively (more than 10 times) washed B. thuringiensis subsp. kurstaki crystals.…”

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confidence: 99%

“…In particular, reports on the carbohydrate content of purified B. thuringiensis subsp. kurstaki crystals range from 0.1 to 12% (4,5,12,13). These differences in reported values may be due to different methods of crystal purification and the limitations of the analytical procedures selected.…”

mentioning

confidence: 99%

Amino sugars in the glycoprotein toxin from Bacillus thuringiensis subsp. israelensis

Pfannenstiel¹,

Muthukumar²,

Couche³

et al. 1987

J Bacteriol

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The subunits of the parasporal crystal of Bacillus thuringiensis: Size, linkage and toxicity

Cited by 87 publications

References 23 publications

A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)

A Toxic Fragment from the Entomocidal Crystal Protein ofBacillus thuringiensis

Amino sugars in the glycoprotein toxin from Bacillus thuringiensis subsp. israelensis

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