The Synergistic Effect of Leukocyte Platelet-Rich Fibrin and Micrometer/Nanometer Surface Texturing on Bone Healing around Immediately Placed Implants: An Experimental Study in Dogs

Abstract

Aims. This study evaluated the effects of L-PRF presence and implant surface texture on bone healing around immediately placed implants. Methods. The first mandibular molars of 8 beagle dogs were bilaterally extracted, and implants (Blossom™, Intra-Lock International, Boca Raton, FL) were placed in the mesial or distal extraction sockets in an interpolated fashion per animal. Two implant surfaces were distributed per sockets: (1) dual acid-etched (DAE, micrometer scale textured) and (2) micrometer/nanometer scale textured (Ossean™ surface). L-PRF (Intraspin system, Intra-Lock International) was placed in a split-mouth design to fill the macrogap between implant and socket walls on one side of the mandible. The contralateral side received implants without L-PRF. A mixed-model ANOVA (at α = 0.05) evaluated the effect of implant surface, presence of L-PRF, and socket position (mesial or distal), individually or in combination on bone area fraction occupancy (BAFO). Results. BAFO values were significantly higher for the Ossean relative to the DAE surface on the larger mesial socket. The presence of L-PRF resulted in higher BAFO. The Ossean surface and L-PRF presence resulted in significantly higher BAFO. Conclusion. L-PRF and the micro-/nanometer scale textured surface resulted in increased bone formation around immediately placed implants.

Effects of Autologous Platelet-Rich Fibrin in Post-Extraction Alveolar Sockets: A Randomized, Controlled Split-Mouth Trial in Dogs with Spontaneous Periodontal Disease

“…The clinical application of PRF in bone defects and extraction sites is also related to its ability to function as a "competitive barrier"; as the soft tissue regenerates faster than the hard tissue, the use of this hemo-component allows the periosteum to be strengthened by acting as an interface between these two types of tissues, avoiding the migration of the soft tissue at depth inside the bone defect [59]. In particular, in a study carried out in dogs, the ability of the PRF to prevent the migration of soft tissue within the extraction site when it was positioned around the implant was highlighted, demonstrating the potential of PRF to act as a physical barrier to avoid soft tissue downgrowth in the socket, resulting in increased bone formation [49].…”

“…In some studies, the PRF has been tested on the healing of canine extraction sockets and in peri-implant sites, however all of these studies were experimental; therefore, lesions and tooth extractions were specially created in healthy dogs, which were used animal models for preclinical purposes [45][46][47][48][49][50][51][52][53].…”

Biomimetic Implant Surface Functionalization with Liquid L-PRF Products: In Vitro Study

“…The authors report increases in the rate and the amount of new bone formation in the experimental group compared to the control, especially in the early healing stages. In a histologic study in dogs, Neiva et al [ 20 ] evaluated the effects of L-PRF around immediately placed implants with two different surfaces, micro/nano-rough surface (Ossean™ surface) and dual-etched. The authors reported a significantly increased bone formation by combining Ossean surface with L-PRF concluding that micro/nano-rough surface and L-PRF have a synergistic effect on peri-implant bone healing.…”

The use of bioactive factors to enhance bone regeneration: A narrative review

“…In immediately placed implants in dogs, the addition of L‐PRF in the socket resulted in significantly increased bone formation compared to empty sockets (Neiva, Gil, Tovar, Janal & Marao, ). Moreover, in immediately placed implants with a 4X4 defect, combining umbilical cord MSCs with PRF resulted in significantly increased BIC and newly formed bone compared with the use of PRF alone (Hao, Wang, Xu, Xu & Li, ).…”