The targeted inhibition of prostate cancer by iron-based nanoparticles based on bioinformatics

Abstract

Prostate cancer is an epithelial malignant tumor of the prostate, and it is one of the malignant tumors with a high incidence of urogenital system in men. The local treatment of prostate cancer is mainly radical resection and radical radiotherapy, but they are not applicable to advanced prostate cancer. Systemic therapy mainly includes targeted therapy and immunotherapy which could cause many complications, and will affect the prognosis and quality of life of patients. It is urgent to find new treatments for prostate cancer. Bioinformatics offers hope for us to find reliable therapeutic targets. Bioinformatics can use the tumor informations in database and analyze them to screen out the best differentially expressed genes. Using the selected differentially expressed genes as targets, a gene interference plasmid was designed, and the constructed plasmid was used for targeted gene therapy. There are some problems about gene therapy that need to be solved, such as how to transfer genes to target cells is also an important challenge. Due to their large molecular weight and hydrophilic nature, they cannot enter cells through passive diffusion mechanisms. Here we synthesized a DNA carrier used surface modified iron based nanoparticles, and used it to load plasmid including ShRNA which can inhibit the expression of oncogene SLC4A4 selected by bioinformatics’ method. After that we use this iron based nanoparticles/plasmid DNA nanocomposite to treat prostate cancer cells in vitro and in vivo. The target gene SLC4A4 we had selected using bioinformatics had a strong effect on the proliferation of prostate cells; Our nanocomposite could inhibit the expression of SLC4A4 effectively, it had strong inhibitory effects on prostate cancer cells both in vivo and in vitro, and can be used as a potential method for prostate cancer treatment.

Nanotherapy to Reshape the Tumor Microenvironment: A New Strategy for Prostate Cancer Treatment

“…These nanocomposites effectively inhibited the expression of SLC4A4 and demonstrated potent inhibitory effects on PCa cells in vivo and in vitro, demonstrating their potential as therapeutic strategies for the treatment of PCa. 148 In vivo studies have shown that NPs with good biocompatibility can significantly eradicate prostate tumors. GRPr-mediated photothermal thermodynamics combined with an antiapoptotic mechanism can be used to treat PCa.…”

“…These nanocomposites effectively inhibited the expression of SLC4A4 and demonstrated potent inhibitory effects on PCa cells in vivo and in vitro , demonstrating their potential as therapeutic strategies for the treatment of PCa. 148 …”

Intranasal Delivery of Carvedilol- and Quercetin-Encapsulated Cationic Nanoliposomes for Cardiovascular Targeting: Formulation and In Vitro and Ex Vivo Studies

“…7 A study revealed that QC enhances the protective effect of CVD against renal ischemia-reperfusion injury. 24 Globally, researchers have explored and reported the theranostic benefits of various novel drug delivery systems involving nanoparticles (metal or polymeric), 25,26 microspheres, 27 hydrogels, 28 nanosuspensions, 29 emulsions, 30 and lipid-based nanocarriers (NLCs). 31 Lipid-based carrier systems are one of the most explored delivery carriers now-a-days because of their biocompatibility and therefore could enhance the solubility and bioavailability of BCS class II and class IV categoric drugs.…”