The Thyrotrophin Receptor in Guinea-Pig Thyroid Homogenate: Interaction With the Long-Acting Thyroid Stimulator

Abstract

1974): Correlation between long~acting thyroid stimulator protector level and thyroid 131 I uptake in thyrotoxicosis. Brit. me d. 1974): Stimulation of the human thyroid by infusions of plasma containing LATS protector, J. c/in. Endocr. 39,826. 7. McKENZIE, J. M. (1968): Delayed thyroid response to serum from thyrotoxic patients. Endocrinology 62, 865. 8. ADAMS, D. D. ( 1958): The presence of an abnormal thyroid-stimulating hormone in the serum of some thyrotoxic patients, J. c/in. Endocr. 18, 699. 9. KNIGHT, A. and ADAMS, D. D. (1973): Infusion of LATS protector and LATS into monkeys, Proc. Univ. Otago med. Sch. 51, I I. 10. WALL, J. R., GOOD, B. F. and HETZEL, B. S. (1969): Long-acting thyroid stimulator in euthyroid relatives of thyrotoxic patients, Lancet 2, I 024. I I. CHOPRA, I. : A radio-ligand receptor assay for the long-acting thyroid stimulator, Biochem. J. 14!, 105. 15. KRISS, J.P., PLESHAKOV, V. and CHIEN, J. R. (1964): Isolation and identification of the long-acting thyroid stimulator and its relation to hyperthyroidism and circumscribed pretibial myxoedema, J. c/in. Endocr. 24, 1005. 16. 0DDJE, T. H., MESCHAN, I. and WORTHAM. J. (1955): Thyroid function assay with radioiodine. II. Routine calculation· of thyroidal and renal rate factors,

Effects of Autoantibodies to the Insulin Receptor on Isolated Adipocytes

“…During this terminal phase of her disease, circulating levels of 6 The effects of anti-insulin receptor antibodies on insulin binding and glucose oxidation are very similar to the effects reported for immunoglobulin fractions from patients with Graves' disease. Several laboratories have now reported that serum and immunoglobulin fractions from patients with Graves' disease can inhibit the binding of labeled thyroid-stimulating hormone (TSH) to thyroid membranes (23,24,37,38). In addition, these same preparations stimulate the adenylate cyclase activity in thyroid tissue (23,24,(39)(40)(41) and, when administered in vivo, stimulate thyroid hormone secretion (42,43).…”

The interaction of graves' IgG with the thyrotrophin receptor

“…containing -50 mg protein [8] and 1 ml solubilised receptors contained -1 mg protein [9]. Highly purified bovine TSH (30 units/mg), a generous gift from Dr J. G. Pierce, was labelled with "'1 using the iodogen method [lo] and receptor purified [2,3,11]. Of the labelled material, 65-75% could be bound by an excess of thyroid membranes and showed similar biological activity to the native material [ 111.…”

Accuracy of receptor-based methods for detection of thyrotropin-receptor autoantibodies: a new automated third-generation immunoassay shows higher analytical and clinical sensitivity for the differential diagnosis of hyperthyroidism

“…Following early seminal experiments [6,7], Rees Smith and Hall in the early 1980s were the first to describe a competitive receptor immunoassay with inhibition of TSH binding on the TSHR [8,9]. Further modifications of the analytical procedure and the commercial availability of reagents have made this assay the method of choice for TRAb measurement in most clinical laboratories [10,11].…”