The ultrastructure of centriole in mammalian tissue culture cells

Vorobjev, Ivan A.; Chentsov, Yu. S.

doi:10.1016/0309-1651(80)90177-0

Cited by 45 publications

(36 citation statements)

References 3 publications

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“…At the site where the centriole transforms into a cilium, there is no basal plate that is typical of kinocilia . The appendages locate strictly symmetrically, compared with the cilum-free centrioles the structure of which we described previously (34).…”

Section: Resultsmentioning

confidence: 58%

“…Selected interphase cells were identified in light micrographs. Rotational photos were prepared as described previously (34) . To measure the centriole tilt towards the section plane, we made, for comparison, photos of the perpendicular section of the centriole using a microscope with a goniometer (Philips-401) at various tilt angles of the section plane.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Centrioles in the cell cycle. I. Epithelial cells.

Vorobjev¹,

YuS²

1982

The Journal of Cell Biology

341

317

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A study was made of the structure of the centrosome in the cell cycle in a nonsynchronous culture of pig kidney embryo (PE) cells. In the spindle pole of the metaphase cell there are two mutually perpendicular centrioles (mother and daughter) which differ in their ultrastructure . An electron-dense halo, which surrounds only the mother centriole and is the site where spindle microtubules converge, disappears at the end of telophase. In metaphase and anaphase, the mother centriole is situated perpendicular to the spindle axis . At the beginning of the G, period, pericentriolar satellites are formed on the mother centriole with microtubules attached to them ; the two centrioles diverge. The structures of the two centrioles differ throughout interphase ; the mother centriole has appendages, the daughter does not. Replication of the centrioles occurs approximately in the middle of the S period . The structure of the procentrioles differs sharply from that of the mature centriole. Elongation of procentrioles is completed in prometaphasé, and their structure undergoes a number of successive changes. In the G2 period, pericentriolar satellites disappear and some time later a fibrillar halo is formed on both mother centrioles, i .e ., spindle poles begin to form . In the cells that have left the mitotic cycle (Go period), replication of centrioles does not take place; in many cells, a cilium is formed on the mother centriole. In a small number of cells a cilium is formed in the S and G2 periods, but unlike the cilium in the Go period it does not reach the surface of the cell . In all cases, it locates on the centriole with appendages . At the beginning of the G, period, during the G2 period, and in nonciliated cells in the Go period, one of the centrioles is situated perpendicular to the substrate. On the whole, it takes a mature centriole a cycle and a half to form in PE cells.The behavior of centrioles in the cell cycle has been described in a number of works (13,21,22,24,29,30), but the majority of authors have confined themselves to a description of some individual cycle phases rather than making a comprehensive analysis of all the periods of interphase and mitosis . The emphasis in these works is on the location of centrioles in the cell and on the time of their duplication, whereas the interaction between interphase centrioles and cytoplasmic microtubules and the dynamics of all centrosome structures are hardly mentioned . Very little is known about the structure of the growing centriole in unciliated cells from the moment of its emergence near the mother centriole. Of great interest is the verification of the assumption about the oriented location of centrioles in mitosis and interphase.In the present work we have tried to elucidate the changes in centriole structure and general composition of the centriolar apparatus during and at completion of the mitotic cycle. The method was essentially as follows: we marked the individual cell under the light microscope and then examined the serial sections of this ...

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Section: Resultsmentioning

confidence: 58%

Section: Methodsmentioning

confidence: 99%

Centrioles in the cell cycle. I. Epithelial cells.

Vorobjev¹,

YuS²

1982

The Journal of Cell Biology

341

317

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“…Stubblefield and Brinkley (Stubblefield and Brinkley, 1967) described a structure in the distal lumen in centrioles in Chinese hamster fibroblasts that did not exhibit ninefold rotational symmetry. Vorobjev and Chentsov (Vorobjev and Chentsov, 1980) found a fine filament that formed an incomplete circle at the distal end of centrioles in various mammalian species. In the latter two studies, the acorn is not clearly revealed.…”

Section: Discussionmentioning

confidence: 99%

The ultrastructure of theChlamydomonas reinhardtiibasal apparatus: identification of an early marker of radial asymmetry inherent in the basal body

Geimer

Melkonian

2004

Journal of Cell Science

135

193

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The biflagellate unicellular green alga Chlamydomonas reinhardtii is a classic model organism for the analysis of flagella and their organizers, the basal bodies. In this cell, the two flagella-bearing basal bodies, along with two probasal bodies and an array of fibers and microtubules, form a complex organelle called the basal apparatus. The ultrastructure of the basal apparatus was analysed in detail by serial thin-section electron microscopy of isolated cytoskeletons and several newly discovered features are described, including a marker for the rotational asymmetry inherent in the basal bodies and probasal bodies. In addition, the complex three-dimensional basal apparatus ultrastructure is resolved and illustrated, including the attachment sites of all basal apparatus elements to specific microtubular triplets of the basal bodies and probasal bodies. These data will facilitate both the localization of novel basal apparatus proteins and the analysis of mutants and RNA interference cells with only subtle defects in basal apparatus ultrastructure. The early harbinger of radial asymmetry described here could play a crucial role during basal body maturation by orienting the asymmetric attachment of the various associated fibers and therefore might define the orientation of the basal bodies and, ultimately, the direction of flagellar beating.

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“…The mother centriole defines the site of nascent CBB assembly, the position and orientation of cilia formation and beating, the orientation of mitotic spindle poles and the maintenance of cellular geometry. During mitosis, the mother centriole is positioned within the centrosome perpendicular to the spindle axis, suggesting that the asymmetric orientation of centrioles is important for mitotic chromosome segregation (Vorobjev and Chentsov, 1980). Furthermore, proper positioning and orientation of motile cilia is required for effective ciliary beating to move fluid.…”

Section: Introductionmentioning

confidence: 99%

Choosing sides – asymmetric centriole and basal body assembly

Pearson

2014

Journal of Cell Science

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Centrioles and basal bodies (CBBs) are microtubule-rich cylindrical structures that nucleate and organize centrosomes and cilia, respectively. Despite their apparent ninefold rotational symmetry, the nine sets of triplet microtubules in CBBs possess asymmetries in their morphology and in the structures that associate with them. These asymmetries define the position of nascent CBB assembly, the orientation of ciliary beating, the orientation of spindle poles and the maintenance of cellular geometry. For some of these functions, the orientation of CBBs is first established during new CBB biogenesis when the daughter structure is positioned adjacent to the mother. The mother CBB organizes the surrounding environment that nascent CBBs are born into, thereby providing a nest for the new CBB to develop. Protists, including ciliates and algae, highlight the importance of this environment with the formation of asymmetrically placed scaffolds onto which new basal bodies assemble and are positioned. Recent studies illuminate the positioning of nascent centrioles relative to a modular pericentriolar material (PCM) environment and suggest that, like ciliates, centrosomes organize an immediate environment surrounding centrioles for their biogenesis and positioning. In this Commentary, I will explore the positioning of nascent CBB assembly as the first event in building cellular asymmetries and describe how the environment surrounding both basal bodies and centrioles may define asymmetric assembly.

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The ultrastructure of centriole in mammalian tissue culture cells

Cited by 45 publications

References 3 publications

Centrioles in the cell cycle. I. Epithelial cells.

Centrioles in the cell cycle. I. Epithelial cells.

The ultrastructure of theChlamydomonas reinhardtiibasal apparatus: identification of an early marker of radial asymmetry inherent in the basal body

Choosing sides – asymmetric centriole and basal body assembly

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