The use of a commercial test system (SALMOTYPE ELISA) for tracing antibodies against Salmonella in the serum of pigs

Gabert, Jörg; Schalch, B.; Greil, B.; Sperner, Brigitte; Stolle, A.; Weber, Cornelia; Krämer, Thomas

doi:10.31274/safepork-180809-962

Cited by 6 publications

(3 citation statements)

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“…Salmonellosis is among the food-borne diseases most widespread global public health problems, and their implication for health and economy is increasingly recognized (Van der Venter, 1999). According to the World Health Organization reports of 1995, 88% of all food-borne diseases were caused by Salmonella (Gabert et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Evaluating the relative resistance of different poultry breeds to Salmonella Typhimurium

Girmay¹,

Dessie²,

Sissay³

et al. 2015

Afr. J. Agric. Res.

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Salmonella infections originating from poultry are one of the major causes of food-borne diseases. To control Salmonella in poultry multifactorial approach is likely to be effective, and genetic resistance of poultry breeds to Salmonella infections may have valuable contribution. Experimental research was conducted to evaluate relative resistance of poultry breeds: Local, Fayoumi and Koekoek to Salmonella Typhimurium from November 2012 to May 2013. Total of 48 chicks were taken and half from each was randomly allocated to treatment and control groups. Treatment groups were orally infected with 10 7-9 S. Typhimurium at two week's age. Samples were taken from week one post infection and continued every week till week four. Liver, spleen and lung were considered to determine relative bacterial loads and feces were added to identify S. Typhimurium. Highest bacterial load was found in Koekoek (1.95 log CFU/ gram) breeds than Local (0.71 log CFU/ gram) and Fayoumi (1.00 log CFU/ gram) breeds (P<0.05). However, no statistically significant difference of bacterial load between Local and Fayoumi was found. There was significant bacterial load difference among liver (2.01 log CFU/ gram), lung (0.95 log CFU/ gram) and spleen (0.69 log CFU/ gram). According to bacterial growth on XLD, Koekoek was found to be 4.26 times more susceptible to Local breed. There was no statistically significant difference between Local and Fayoumi breeds. In conclusion, local breeds were relatively found to be the most resistant among the three poultry breeds to S. Typhimurium.

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Section: Introductionmentioning

confidence: 99%

Evaluating the relative resistance of different poultry breeds to Salmonella Typhimurium

Girmay¹,

Dessie²,

Sissay³

et al. 2015

Afr. J. Agric. Res.

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“…The herd monitoring scheme makes use of the SALMOTYPE®‐ELISA, a test based on the so‐called Danish mix ELISA test (LPS antigens 0:1, 4, 5, 6, 7, 12), licensed and commercially available from Labor‐Diagnostik (Leipzig, Germany; Gabert et al., 1999). When starting the programme, the ‘cut‐off value’ (more than 40% serum titre of antibodies against Salmonella for positive and >20 ≤ 40% for doubtful samples) was specified according to the guidelines for a programme aimed at reducing Salmonella transmission into meat production by way of slaughter pigs as defined by the German Federal Ministry for Food, Agriculture and Forestry.…”

Section: Methodsmentioning

confidence: 99%

The Styrian Salmonella Monitoring Programme for Pork Production

Köfer

Kleb

Pless

2006

Journal of Veterinary Medicine, Series B

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The Styrian Salmonella Monitoring Programme for pork production is based on a representative analysis of the current status, serological meat juice monitoring and bacteriological tests of carcass halves and parts and has been in operation since 1999. A total of 34 170 meat juice samples from 3417 finisher herds were tested using the meat juice SALMOTYPE-ELISA (Labor Diagnostik, Leipzig, Germany) in the period from 1999 to 2003. More than 95% of the samples investigated were below the negative cut-off of <20% based on the 5-year average. The mean extinction values for meat juice samples showed regional differences, which were visualized for epidemiological purposes using the VETGIS geographical information system (Department of Veterinary Administration, Graz, Austria). Salmonella spp. were detected in only 15 cases (0.13%) of a total of 11 330 bacteriologically tested wipe samples from meat-processing plants. The Salmonella isolates detected included four S. Typhimurium, two S. Enteritidis PT 4, five S. Infantis, one S. Bredeny, one S. Saintpaul, one S. Brenderup and one S. Livingstone isolates. The proportion of Salmonella-contaminated pork in the total population estimated from the annual sample showed a falling tendency. It decreased from 0.48% (CI: 0.23 < or = P < or = 0.85) in 1999 to 0.14% (CI: 0.07 < or = P < or = 0.24) in 2003. The contamination of Styrian pork with Salmonella is extremely low and thus poses a negligible risk of infection to consumers.

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“…The Netherlands initiated a sero-surveillance program for finishing pigs as of January 2005. This can be done by testing pig sera by ELISA for the presence of antibodies against Salmonella Spp in sera and/or meat drip (Nielsen et al, 1995, Van der Heijden et al, 1995, Gabert et al, 1999, Proux et al, 2000. In this paper we describe the validation of a novel test, based on SPR that detects porcine Salmonella antibodies directed to sero-groups B and D. Validation of the Salmonella SPR assay has been focused on test characteristics that determine the validity (specificity and sensitivity).…”

mentioning

confidence: 99%

Validation of a surface plasmon resonance based assay to detect Salmonella antibodies in serium of pigs

Swanenburg¹,

Bloemraad²,

Achterberg³

et al. 2005

International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

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The aim of this study was to develop and validate a novel serological test employing surface plasmon resonance (SPR), that was designed for use on the Biacore Q apparatus to detect antibodies against Salmonella Spp in pig sera. Salmonella Spp antigens (sero-groups B and D) were immobilized onto CM-5 sensor chips (Biacore). Test conditions and apparatus adjustments were optimized. Subsequently, validation was done using positive and negative ("in house") reference sera as well as 990 field sera collected at slaughter. A set of 990 sera originating from finishing pigs were tested in the SPR assay and ELISA. Overall agreement between the two tests amounted to 92%. From these results it is concluded that the SPR assay in conjunction with the Biacore Q apparatus is suitable for screening slaughter pigs against Salmonella in a surveillance program.

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The use of a commercial test system (SALMOTYPE ELISA) for tracing antibodies against Salmonella in the serum of pigs

Cited by 6 publications

References 0 publications

Evaluating the relative resistance of different poultry breeds to Salmonella Typhimurium

Evaluating the relative resistance of different poultry breeds to Salmonella Typhimurium

The Styrian Salmonella Monitoring Programme for Pork Production

Validation of a surface plasmon resonance based assay to detect Salmonella antibodies in serium of pigs

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