The use of antibiotic resistance profiling as a means of tracing sources of fecal contamination in source waters

Mthembu, Mathews Simon; Biyela, PT; Djarova, Trayana; Basson, Albertus K.

doi:10.2166/ws.2010.228

Cited by 4 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because these types of studies often fail to compare sites based on physical distance or direction from the source (e.g. (Edge and Hill, 2005; Dickerson et al , 2007; Mthembu et al , 2010; Murugan et al , 2012)), such studies do not provide evidence for this systematic review question. Any study that did not meet all these criteria was excluded.…”

Section: Methodsmentioning

confidence: 99%

Impact of point sources on antibiotic resistance genes in the natural environment: a systematic review of the evidence

Bueno

Williams-Nguyen

Hwang

et al. 2017

Anim. Health. Res. Rev.

View full text Add to dashboard Cite

There is a growing concern about the role of the environment in the dissemination of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG). In this systematic review, we summarize evidence for increases of ARG in the natural environment associated with potential sources of ARB and ARG such as agricultural facilities and wastewater treatment plants. A total of 5247 citations were identified, including studies that ascertained both ARG and ARB outcomes. All studies were screened for relevance to the question and methodology. This paper summarizes the evidence only for those studies with ARG outcomes (n = 24). Sixteen studies were at high (n = 3) or at unclear (n = 13) risk of bias in the estimation of source effects due to lack of information or failure to control for confounders. Statistical methods were used in nine studies; three studies assessed the effect of multiple sources using modeling approaches, and none reported effect measures. Most studies reported higher ARG concentration downstream/near the source, but heterogeneous findings hindered making any sound conclusions. To quantify increases of ARG in the environment due to specific point sources, there is a need for studies that emphasize analytic or design control of confounding, and that provide effect measure estimates.

show abstract

Section: Methodsmentioning

confidence: 99%

Impact of point sources on antibiotic resistance genes in the natural environment: a systematic review of the evidence

Bueno

Williams-Nguyen

Hwang

et al. 2017

Anim. Health. Res. Rev.

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…It is evident from the data gathered herein that the seven WRPs investigated herein were mere dumping sites for the community's wastewater. In this context, the WRPs potentially present a severe potential threat to public health (Mthembu et al, 2010). The WRPs pool the community's wastewater and are typically much closer to residences than open defecation sites have been in the past.…”

Section: Wrps Fail To Treat Domestic Wastewatermentioning

confidence: 99%

Elevated levels of anthropogenic antibiotic resistance gene marker, sul1, linked with extreme fecal contamination and poor water quality in wastewater‐receiving ponds

2023

View full text Add to dashboard Cite

In several low‐ and middle‐income countries, such as India, the rapid construction of toilets to combat open defecation has not been matched with adequate wastewater treatment, resulting in extreme fecal contamination of the receiving environments. The sewage‐receiving surface water bodies, typically close to the residences, are a potential hotspot for disease transmission and antibiotic resistance. Water, soil, and sediment samples from seven wastewater‐receiving ponds (WRPs) were analyzed for water quality, chlorophyll‐a, fecal contamination (yccT for Escherichia coli), 16S rRNA gene copies, and anthropogenic antibiotic resistance gene markers—sul1 and intI1. These WRPs were contrasted with two ponds that did not directly receive sewage. The water quality in the WRPs was comparable to raw sewage (BOD: 210–380 mg/L; COD: 350–630 mg/L; total‐N: 100–190 mg/L; and total‐P: 6–21 mg/L), and the relative levels of the DNA marker of E. coli were very high (yccT: 0.1% to ∼100% of total bacterial count) indicating extreme fecal contamination. The relative levels of sul1 and intI1 were 1–3 orders of magnitude higher in WRPs (sul1: 0.32%–10% of total bacterial count; and intI1: 0.2%–5% of total bacterial count) compared to the ponds that did not receive sewage directly. The relative levels of sul1 correlated with the DNA marker for the fecal indicator, E. coli (p‐value < 0.05; r = 0.50; Spearman's rank correlation), and poor water quality.

show abstract

“…These types of studies often do not explicitly compare sites based on physical distance or direction from the source (e.g. Dickerson, Hagedorn, & Hassall, ; Edge & Hill, ; Mthembu, Biyela, Djarova, & Basson, ; Murugan, Prabhakaran, Al‐Sohaibani, & Sekar, ). As a result, such studies do not provide evidence for this systematic review question.…”

Section: Methodsmentioning

confidence: 99%

Systematic Review: Impact of point sources on antibiotic‐resistant bacteria in the natural environment

Bueno

Williams-Nguyen

Hwang

et al. 2017

Zoonoses and Public Health

View full text Add to dashboard Cite

SummaryPoint sources such as wastewater treatment plants and agricultural facilities may have a role in the dissemination of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARG). To analyse the evidence for increases in ARB in the natural environment associated with these point sources of ARB and ARG, we conducted a systematic review. We evaluated 5,247 records retrieved through database searches, including both studies that ascertained ARG and ARB outcomes. All studies were subjected to a screening process to assess relevance to the question and methodology to address our review question. A risk of bias assessment was conducted upon the final pool of studies included in the review. This article summarizes the evidence only for those studies with ARB outcomes (n = 47). Thirty-five studies were at high (n = 11) or at unclear (n = 24) risk of bias in the estimation of source effects due to lack of information and/ or failure to control for confounders. Statistical analysis was used in ten studies, of which one assessed the effect of multiple sources using modelling approaches; none reported effect measures. Most studies reported higher ARB prevalence or concentration downstream/near the source. However, this evidence was primarily descriptive and it could not be concluded that there is a clear impact of point sources on increases in ARB in the environment. To quantify increases in ARB in the environment due to specific point sources, there is a need for studies that stress study design, control of biases and analytical tools to provide effect measure estimates. K E Y W O R D Santibiotic-resistant bacteria, effect measure, environmental dissemination, point source, systematic review

show abstract

The use of antibiotic resistance profiling as a means of tracing sources of fecal contamination in source waters

Cited by 4 publications

References 0 publications

Impact of point sources on antibiotic resistance genes in the natural environment: a systematic review of the evidence

Impact of point sources on antibiotic resistance genes in the natural environment: a systematic review of the evidence

Elevated levels of anthropogenic antibiotic resistance gene marker, sul1, linked with extreme fecal contamination and poor water quality in wastewater‐receiving ponds

Systematic Review: Impact of point sources on antibiotic‐resistant bacteria in the natural environment

Contact Info

Product

Resources

About