The Use of BEREP4 Immunohistochemistry Staining for Detection of Basal Cell Carcinoma

Sunjaya, Anthony Paulo; Tan, Sukmawati Tansil

doi:10.1155/2017/2692604

Cited by 35 publications

(26 citation statements)

References 43 publications

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“…Of note, the pattern of TG3 expression is similar to that of Ep-CAM, specifically found in this type of skin cancer. In fact, routinely, BCC diagnosis is also confirmed by positive Ep-CAM staining [43]. Ep-CAM is absent in normal skin, and negative staining for Ep-CAM in tissue could be due to inappropriate fixation or processing of the samples, which may lead to uncertain conclusions regarding the diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Transglutaminase 3 is expressed in basal cell carcinoma of the skin

Smirnov¹,

Anemona²,

Montanaro³

et al. 2019

European Journal of Dermatology

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Transglutaminase 3 is expressed in basal cell carcinoma of the skin Background: Transglutaminase 3 (TG3) belongs to a family of Ca 2+dependent enzymes which catalyse protein crosslinking. TG3 is important for proper development of the skin and hair shaft, and knockout mice for the Tgm3 gene are sensitive to UVB-induced photodamage due to aberrations in cornified envelope formation. Loss of TG3 is reported in head and neck and oesophageal squamous cell carcinoma, yet, its expression in skin cancer has not been studied. Objectives: The aim of the present study was to analyse the expression pattern of TG3 in skin cancer. Materials and methods: TG3 expression was investigated based on immunohistochemical staining of a tissue micro-array of different types of skin cancer, as well as meta-analysis of public gene array data. Results: Our findings demonstrated that TG3 is normally expressed in spinous/granular layers of the epidermis, but is absent in melanocytes as well as melanoma samples. As expected, its expression was absent in poorly differentiated squamous cell carcinoma of the skin. Surprisingly, we show that samples of basal cell carcinoma demonstrated strong staining for TG3 both in the cytoplasm and nucleus. Furthermore, at the mRNA level, the expression pattern of TGM3 was crucially altered in BCC, but not other types of skin cancer. Conclusion: These findings lead to new questions regarding TG3 involvement in basal cell carcinoma tumourigenesis. Moreover, the expression pattern of TG3 renders it a potential specific marker for basal cell carcinoma diagnosis.

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Section: Discussionmentioning

confidence: 99%

Transglutaminase 3 is expressed in basal cell carcinoma of the skin

Smirnov¹,

Anemona²,

Montanaro³

et al. 2019

European Journal of Dermatology

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“…In order to ensure that the earliest BCC specimens are used, the monoclonal antibody, BerEP4, immunohistochemistry staining was done. BerEP4 shows strong positive result at the cytoplasm and the membrane of the all BCC, anagen hair follicle, sebaceoma, eccrine glands, and the basosquamous carcinomas of the skin [10, 11, 24, 25]. …”

Section: Discussionmentioning

confidence: 99%

Basal Cell Carcinoma Arises from Interfollicular Layer of Epidermis

Tan

Ghaznawie

Heenan³

et al. 2018

Journal of Oncology

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Background BCC is currently the most common type of skin cancer in humans. Although having a low-grade malignancy and metastatic potential, BCC is locally aggressive and destructive. Despite numerous studies, the origin of BCC, whether arising from the follicular or interfollicular layer, remains controversial. Objectives This study aims to evaluate whether BCC arises from the follicular or interfollicular layer by using immunohistochemical staining. Methods Twenty-three specimens of superficial and nodular BCC at its very early stage were examined. The samples were immunohistochemically stained using BerEP4 antibody. The stained specimens were then examined and scored by 2 independent observers. Results BerEP4 was found to be strongly positive in all BCC lesions, including a very early lesions budding off the basal layer of the epidermis. Conclusion This study confirmed that the origin site of BCC is basal layer of epidermis. This finding suggests that BCC arises from the interfollicular epidermis.

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“…Positive staining with antibodies targeting BerEP4, which marks the epithelial cell adhesion molecule, has been proved useful to differentiate BCC from SCC. 4 The present case demonstrates that, although rare, BCC should be included in the differential diagnoses of solitary palmar lesions.…”

Section: Supporting Informationmentioning

confidence: 64%