The use of live‐animal micro‐computed tomography to determine the effect of a novel phospholipase A<sub>2</sub> inhibitor on alveolar bone loss in an <i>in vivo</i> mouse model of periodontitis

Cantley, Melissa; Bartold, P. Mark; Marino, Victor; Reid, Robert C.; Fairlie, David P.; Wyszynski, R. N.; Zilm, Peter; Haynes, David R.

doi:10.1111/j.1600-0765.2008.01132.x

Cited by 27 publications

(36 citation statements)

References 24 publications

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“…The preparation of the inocula and the inoculation protocol to induce an experimental periodontitis in female BALB/c mice has been previously described [22]. All mice were inoculated with either wild type P. gingivalis (Groups 3 and 6) or PAD-deficient P. gingivalis (Groups 2 and 5).…”

Section: Methodsmentioning

confidence: 99%

“…The scanning width was set to 35 mm with a resolution of 9 µm. Detailed specifications for micro CT imaging have previously been published [22]. Animals were anaesthetised prior to scanning with a mixture of ketamine and xylazine and positioned on a polystyrene foam holder then placed within an enclosed container with a HEPA filter at the ends.…”

Section: Methodsmentioning

confidence: 99%

“…The bone volume was calculated in a set number (200) 9 µm slices above and below a standard reference point set within the radiocarpal joint. The CEJ-ABC distance was measured between the second and third molars on three slices for each mouse [22], [25].…”

Section: Methodsmentioning

confidence: 99%

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Porphyromonas gingivalis Peptidylarginine Deiminase, a Key Contributor in the Pathogenesis of Experimental Periodontal Disease and Experimental Arthritis

et al. 2014

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ObjectivesTo investigate the suggested role of Porphyromonas gingivalis peptidylarginine deiminase (PAD) in the relationship between the aetiology of periodontal disease and experimentally induced arthritis and the possible association between these two conditions.MethodsA genetically modified PAD-deficient strain of P. gingivalis W50 was produced. The effect of this strain, compared to the wild type, in an established murine model for experimental periodontitis and experimental arthritis was assessed. Experimental periodontitis was induced following oral inoculation with the PAD-deficient and wild type strains of P. gingivalis. Experimental arthritis was induced via the collagen antibody induction process and was monitored by assessment of paw swelling and micro-CT analysis of the radio-carpal joints. Experimental periodontitis was monitored by micro CT scans of the mandible and histological assessment of the periodontal tissues around the mandibular molars. Serum levels of anti-citrullinated protein antibodies (ACPA) and P. gingivalis were assessed by ELISA.ResultsThe development of experimental periodontitis was significantly reduced in the presence of the PAD-deficient P. gingivalis strain. When experimental arthritis was induced in the presence of the PAD-deficient strain there was less paw swelling, less erosive bone damage to the joints and reduced serum ACPA levels when compared to the wild type P. gingivalis inoculated group.ConclusionThis study has demonstrated that a PAD-deficient strain of P. gingivalis was associated with significantly reduced periodontal inflammation. In addition the extent of experimental arthritis was significantly reduced in animals exposed to prior induction of periodontal disease through oral inoculation of the PAD-deficient strain versus the wild type. This adds further evidence to the potential role for P. gingivalis and its PAD in the pathogenesis of periodontitis and exacerbation of arthritis. Further studies are now needed to elucidate the mechanisms which drive these processes.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Porphyromonas gingivalis Peptidylarginine Deiminase, a Key Contributor in the Pathogenesis of Experimental Periodontal Disease and Experimental Arthritis

et al. 2014

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“…The oral gavage model consists in the inoculation of human bacterial strains of a predefined amount of bacterial in a viscous suspension [18]. Oral gavage utilize mainly P. gingivalis [16,17,21,[26][27][28], A. actinomycetemcomitans [6,29], F. nucleatum [9,17,21], as well as a combination of different strains of bacteria [7-9, 15, 30, 31]. The duration of the experiments vary from 10 days [32] to up to 8 weeks [9,21], differing in the levels of gingival epithelial ulceration, in the levels of inflammatory cytokines [17,21], and in the downgrowth of bone loss [9,21,[32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Long-term evaluation of oral gavage with periodontopathogens or ligature induction of experimental periodontal disease in mice

et al. 2015

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The ligature model was an effective approach to induce inflammation and bone loss similar to human periodontitis, but the oral gavage models were not efficient in inducing periodontal inflammation and tissue destruction in the conditions studied. Ligature models can provide a basis for future interventional studies that contribute to the understanding of the disease pathogenesis and the complex host response to microbial challenge.

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“…Recently, micro-computerized tomography (micro-CT) was used for bone analysis in periodontal research 12,14,27) . Since investigators can follow bone morphological changes in the same animal using micro-CT during the experimental period, it is not necessary to consider individual differences as in section analysis.…”

Section: Introductionmentioning

confidence: 99%

Carrageenin-Induced Periodontitis as an Experimental Model in Rats Analyzed by Micro-Computerized Tomography

Yamamoto

Yokoyama

Tamura³

et al. 2011

J. Hard Tissue Biology.

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Periodontitis is a common disease worldwide and is a cause of tooth loss. For these reasons, many investigators have examined periodontitis not only by in vivo studies but also in vitro studies. For in vivo studies, it is very important which animal is used and how to make experimental periodontitis easily and efficiently in animals. Although the process of periodontitis in monkeys and dogs is similar to that in humans, rats and mice are much more useful animals for experiments because of ease of handling and low cost. For the induction of periodontitis, bacterial inoculation and wire ligature methods were examined previously; however, these methods had temporal and technical problems. Therefore, a simple, easy and reliable experimental model for periodontitis using rats and/or mice is required. In this study, the authors attempted to establish a novel experimental model for periodontitis in the rat lower jaw using carrageenin-immersed silk ligature wire, and examined the change of alveolar bone morphology by micro-computerized tomography. As a result, carrageeninimmersed silk ligature wire induced inflammation of the periodontium with severe alveolar bone loss in all experimental rats. In addition, the degree of absorption of the alveolar bone depended on the dosage times of carrageenin treatment. These findings suggested that this experimental model of periodontitis using carrageenin in rats may be a useful experimental model for periodontal research.

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The use of live‐animal micro‐computed tomography to determine the effect of a novel phospholipase A₂ inhibitor on alveolar bone loss in an in vivo mouse model of periodontitis

Abstract: Live-animal micro-computed tomography is a robust, reproducible technique that clearly demonstrates significant time-dependent changes in alveolar bone volume in a small-animal model of periodontitis.

Cited by 27 publications

References 24 publications

Porphyromonas gingivalis Peptidylarginine Deiminase, a Key Contributor in the Pathogenesis of Experimental Periodontal Disease and Experimental Arthritis

Porphyromonas gingivalis Peptidylarginine Deiminase, a Key Contributor in the Pathogenesis of Experimental Periodontal Disease and Experimental Arthritis

Long-term evaluation of oral gavage with periodontopathogens or ligature induction of experimental periodontal disease in mice

Carrageenin-Induced Periodontitis as an Experimental Model in Rats Analyzed by Micro-Computerized Tomography

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The use of live‐animal micro‐computed tomography to determine the effect of a novel phospholipase A2 inhibitor on alveolar bone loss in an in vivo mouse model of periodontitis

Abstract: Live-animal micro-computed tomography is a robust, reproducible technique that clearly demonstrates significant time-dependent changes in alveolar bone volume in a small-animal model of periodontitis.

Cited by 27 publications

References 24 publications

Porphyromonas gingivalis Peptidylarginine Deiminase, a Key Contributor in the Pathogenesis of Experimental Periodontal Disease and Experimental Arthritis

Porphyromonas gingivalis Peptidylarginine Deiminase, a Key Contributor in the Pathogenesis of Experimental Periodontal Disease and Experimental Arthritis

Long-term evaluation of oral gavage with periodontopathogens or ligature induction of experimental periodontal disease in mice

Carrageenin-Induced Periodontitis as an Experimental Model in Rats Analyzed by Micro-Computerized Tomography

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The use of live‐animal micro‐computed tomography to determine the effect of a novel phospholipase A₂ inhibitor on alveolar bone loss in an in vivo mouse model of periodontitis