The use of Macronet resins to recover γ-decalactone produced by Rhodotorula aurantiaca from the culture broth

Abstract

During the biotransformation of castor oil into gamma-decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of gamma-decalactone of 6.5 g/l was obtained. The parameters of gamma-decalactone adsorption on three Macronet resins (MN-202, MN-102 and MN-100) were investigated in water. Adsorption isotherms of gamma-decalactone for the three Macronet resins were linear. The trapping of gamma-decalactone produced by R. aurantiaca on these resins was then carried out. gamma-Decalactone was effectively retained by all the studied Macronet resins. The resin MN-202 trapped gamma-decalactone more efficiently than MN-102 and MN-100. The percentages of gamma-decalactone adsorbed on the resins MN-202, MN-102 and MN-100 were, respectively, 85, 75 and 81%, whereas around 70% of the adsorbed gamma-decalactone was then desorbed. We propose an industrial process that uses Macronet resins to extract gamma-decalactone from culture broth of R. aurantiaca.

Production of γ‐decalactone by Yarrowia lipolytica: insights into experimental conditions and operating mode optimization

“…Similar results were also reported by Dufossé et al who showed that γ‐decalactone concentration obtained in the culture broth of Sporidiobolus ruinenii increased almost proportionally with ricinoleic acid methyl ester concentration between 0 and 160 g·L ‐1 . Our results contrasted with those reported by Alchihab et al who observed that γ‐decalactone concentration obtained with the strain Rhodotorula aurantiaca was not proportional to the concentration of castor oil added. After 10 days in flasks, the highest γ‐decalactone production, 5.5 g·L ‐1 , was recorded with 20 g·L ‐1 of castor oil.…”

“…Different substrates were described for aroma production such as castor oil and their hydrolysates, fatty acids or esters of these compounds, including methyl ricinoleate, and sugars such as glucose and fructose . The influence of castor oil on the yields of γ‐decalactone was reported by Alchihab et al in flask experiments. The authors showed that γ‐decalactone concentration obtained was not directly proportional to the concentration of castor oil in the medium.…”

Fed-batch versus batch cultures of Yarrowia lipolytica for γ-decalactone production from methyl ricinoleate

“…Nevertheless, the productivity of c-decalactone obtained in the intermittent fed-batch technique was similar to the one obtained by Alchihab et al (2010), which was 45 mg l -1 h -1 with another yeast strain and castor oil.…”

“…Nevertheless, the concentration of both lactones increased along the whole experiment and high amounts of c-decalactone and 3-hydroxy-c-decalactone were achieved with this strategy: 6,798 mg l -1 and 10,018 mg l -1 , respectively, after 173 h of biotransformation. The c-decalactone concentration achieved was slightly higher than the values obtained by Alchihab et al (2010) that used batch cultures of Rhodotorula aurantiaca with Macronet resins to recover the c-decalactone produced, thus reducing the product toxicity effects. Similar behavior describing the product increase until the end of the experiment herein reported was described in other works involving fed-batch cultures (Kim et al 1999;Kyong and Shin 2000;Nicaud et al 2002;Rymowicz et al 2009).…”

Effect of POX genotype and Lip2p overexpression on lactone production and reconsumption by Yarrowia lipolytica using castor oil as substrate

“…Nevertheless, for substrate concentrations higher than 60 g L −1 the production reached a plateau. On the other hand, Alchihab et al [36] observed that ␥-decalactone concentration obtained with the strain Rhodotorula aurantiaca was not proportional to the concentration of castor oil added.…”