The use of mean platelet volume for evaluation of quality of platelet concentrates

Brozović, B.; Seghatchian, M.J.; McShine, R. L.

doi:10.1097/00001721-199210000-00017

Cited by 8 publications

(5 citation statements)

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Section: Discussionsupporting

confidence: 92%

“…Our finding of smaller MPV in samples of PRM concentrates is in complete agreement with the results of others [7,22]. In one study [7] the same type of blood cell counter as we used measured smaller MPV in PRM concentrates than in the full blood from which the concentrates were derived. Brozovic et al [7] thus suggest that in PRM concentrates the larger subpopulation of platelets is selected out by centrifugation together with the erythrocytes during the initial slow centrifugation step of platelet concentrate preparation.…”

Section: Discussionsupporting

confidence: 92%

“…In one study [7] the same type of blood cell counter as we used measured smaller MPV in PRM concentrates than in the full blood from which the concentrates were derived. Brozovic et al [7] thus suggest that in PRM concentrates the larger subpopulation of platelets is selected out by centrifugation together with the erythrocytes during the initial slow centrifugation step of platelet concentrate preparation. Another possible reason for the finding of a higher MPV in BCM concentrates as compared with PRM concentrates is the nature of the suspension medium.…”

Section: Discussionmentioning

confidence: 99%

“…The PF3AT was performed according to the method described by Brozovic et al [7] but with volume adaptation: 0.05 ml of properly diluted platelet concentrate was first incubated for 20 min at 37 7C with 0.05 ml 5 mg/ml kaolin solution for activation of contact coagulation and platelets; afterwards 0.05 ml of 0.025 M CaCl 2 was added and clotting was recorded. The test performance was adapted to the semiautomatic coagulometer ST4 (Stago) equipped with an electromechanical clot detection principle.…”

Section: Measurement Of Concentrate Ppca With Platelet Factor 3 Availmentioning

confidence: 99%

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The evolution of platelet procoagulant activity of remnant platelets in stored platelet concentrates prepared by the platelet-rich plasma method and the buffy coat method

Planken

Vertessen

Mortelmans

et al. 1999

Annals of Hematology

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Platelets stored as concentrates are gradually activated (storage lesion), a process associated with changes in the expression of platelet procoagulant activity (PPCA). The aim of the present study was to evaluate the evolution of PPCA and the mean platelet volume (MPV) of stored platelets prepared according to the platelet-rich method (PRM) and the buffy coat method (BCM). Using the platelet factor 3 availability clotting test (PF3AT) on appropriately diluted concentrate samples, we found a decrease in PPCA expression of remnant platelets as a function of storage time (0.025 < p < 0.01 between day 1 and 7) in PRM-derived but not in BCM-derived platelet concentrates. Using the PF3AT reduction test we found a more important clotting time reduction in samples obtained from BCM than in samples obtained from PRM platelet concentrates, suggesting a higher PPCA expression of BCM platelets, not significant after 1 day but highly significant after 3 days (p < 0.0005) and after 7 days (p < 0.0005) of storage, as compared with PRM platelets. For both PRM and BCM concentrates there were no significant MPV changes as a function of storage time, but at any storage day the MPV of BCM concentrates was significantly higher (p < 0.0005) than the MPV of PRM concentrates. We conclude that the decrease of PPCA expression in PRM-derived concentrates as a function of storage time is in agreement with the gradual decrease of the platelet activation status in PRM concentrates during storage. There are probably several factors or variables causing platelets of BCM concentrates to express higher PPCA than those of PRM concentrates. Higher PPCA expression in BCM concentrates may be explained by an intrinsic platelet property, such as a difference in MPV between the two kinds of concentrates, or it may be related to an extrinsic factor such as different storage media, e.g., undiluted autologous plasma in PRM concentrates versus Plasmalyte A-diluted autologous plasma in BCM concentrates. Whether the difference in PPCA expression of remnant platelets in PRM and BCM concentrates is just an in vitro laboratory finding or may have consequences for the therapeutic efficiency of the concentrates is an interesting, still unresolved question.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Measurement Of Concentrate Ppca With Platelet Factor 3 Availmentioning

confidence: 99%

See 2 more Smart Citations

The evolution of platelet procoagulant activity of remnant platelets in stored platelet concentrates prepared by the platelet-rich plasma method and the buffy coat method

Planken

Vertessen

Mortelmans

et al. 1999

Annals of Hematology

View full text Add to dashboard Cite

show abstract

“…Second, the method of PRP platelet preparation employed involved an in‐line filtration system for leukoreduction, which may have resulted in the preferential loss of larger (younger) platelets through sequestration in the filter and tubing. Conversely, the apheresis procedures may have preferentially collected larger (younger) platelets from the circulating platelet mass of the donor 25 . We did not collect data on mean platelet volume to compare the apheresis and PRP platelet products.…”

Section: Discussionmentioning

confidence: 99%

In vivo recovery and survival of apheresis and whole blood‐derived platelets: a paired comparison in healthy volunteers

et al. 2006

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A new platelet storage lesion index based on paired samples, without and with EDTA and cell counting: Comparison of three types of leukoreduced preparations

Seghatchian¹

2006

Transfusion and Apheresis Science

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The use of mean platelet volume for evaluation of quality of platelet concentrates

Cited by 8 publications

References 0 publications

The evolution of platelet procoagulant activity of remnant platelets in stored platelet concentrates prepared by the platelet-rich plasma method and the buffy coat method

The evolution of platelet procoagulant activity of remnant platelets in stored platelet concentrates prepared by the platelet-rich plasma method and the buffy coat method

In vivo recovery and survival of apheresis and whole blood‐derived platelets: a paired comparison in healthy volunteers

A new platelet storage lesion index based on paired samples, without and with EDTA and cell counting: Comparison of three types of leukoreduced preparations

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