The Use of New Technologies in the Study of Pregnancy Disorders: The OMICS Approach

Visentin, Silvia; Bongiorno, Maria Caterina; Calanducci, Maria; Marin, Loris; Cosmi, Erich

doi:10.17352/2455-2976.000035

Cited by 3 publications

(15 citation statements)

References 31 publications

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“…The insufficiency of a lncRNA transgenic model limits the ability to address the gene-environmental interactions that may result in the highly heterogenous sPTB. Evidence obtained in our earlier studies indicates that lncRNA may be involved in regulating transcription to produce lncRNA-overlapped mRNA and gene-specific mRNA [3,21,22] . In particular, co-differentially expressed pairs of lncRNAs and mRNAs sharing the same genomic loci in sPTB were identified in the ubiquitin-proteasome system (UPS) and found to be related to the ubiquitin-proteasome-collagen (CUP) pathway [23] .…”

Section: Introductionmentioning

confidence: 98%

“…Clinically, sPTB may present as spontaneous preterm labor (sPTL), defined as premature parturition with no prior rupture of fetal membrane, or as preterm premature rupture of fetal membrane (pPROM) that presents as rupture of membrane before uterine contraction. sPTB is a highly heterogeneous syndrome [1,2] , resulting from gene-environment interaction that consequently causes biological changes of (epi)genetic variation and functions as a predisposing factor [3] . Many studies of gene-environment interaction in human sPTB have focused on the determination of a correlation between a monogenic factor, such as a specific genetic locus that is derived from single-omic data generated from genome-wide variation in blood or placenta with the outcome of pregnancy while associated with a specific environmental exposure [1,[4][5][6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that lncRNA is a critical player in regulating epigenetic modification and is thus an important mediator of gene-environment interaction relevant to placental development including trophoblast differentiation [10,11] . In addition to miscarriage (MC), intrauterine grown restriction (IUGR), preeclampsia (PE), and gestational diabetes mellitus (GDM) [12,13] , a pathogenic role of lncRNA is linked to human reproductive disorders, including sPTB [2,3] . LncRNA was found to have an epigenetic regulatory function in the relationship between social and environmental exposures and sPTB [14][15][16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

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Epigenetic impact of long non-coding RNA lnc-ADAM9 on extracellular matrix pathway in preterm syndrome through down-regulation of mRNA-ADAM9

Wang¹,

Liu²,

Hou³

et al. 2023

J Transl Genet Genom

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im: Evidence suggests that the risk of spontaneous preterm birth (sPTB) is the result of environmental exposure interacting with genetic risk and is mediated by epigenetic modification. Long non-coding RNA (lncRNA) comprises a large group of regulators of epigenetic modification that has recently become the focus of increased investigation in reproductive science. Human placenta expresses many lncRNAs, and differential expression profiles (DEPs) have identified several lncRNAs as associated with sPTB. However, little is known about lncRNA’s role in the epigenetic modification of the genes potentially involved in sPTB. This study is to better understand the epigenetic regulation of lncRNA on the development of sPTB. Methods: A transcriptomic analysis of human placentas derived from various pregnancy outcomes was performed as a discovery study. This was followed by a quantitative confirmation to validate the differential transcription of lncADAM9, the lncRNA overlapping with the ADAM9 gene locus, and of lncRNA-overlapped mRNA of ADAM9 (mRNA-ADAM9). In vitro examination of lncADAM9 transgenic (TG) HTR8 cells were used to perform functional assessment to address the role of lncADAM9-mediated epigenetic regulation of extracellular matrix-adhesion (ECM-A) associated molecules. This assessment was then expanded to studies of human fetal membranes. Results: We observed that expression of lncADAM9 was increased in sPTB, and this increase was further associated with the down-regulation of mRNA-ADAM9 in human placentas. In vitro, overexpression of lncADAM9 in lncADAM9-transgenic HRT8 cells led to DEPs relevant to ECM-A molecules, particularly at the loci of CNTN1, NRXN2, SPN, ICAM2, and HLA-DPB1. This was also true in fetal membranes from abnormal versus normal fetal membranes. Conclusion: We have studied the epigenetic impact of differentially expressed lncADAM9 on the ECM-A pathway that is associated with sPTB and documented that this impact may be mediated through the down-regulation of mRNA-ADAM9. Our results of demonstrating the epigenetic regulation of lncADAM9 on the ECM-A pathway may help provide greater insight into critical pathogenic mechanisms underlying sPTB.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Epigenetic impact of long non-coding RNA lnc-ADAM9 on extracellular matrix pathway in preterm syndrome through down-regulation of mRNA-ADAM9

Wang¹,

Liu²,

Hou³

et al. 2023

J Transl Genet Genom

View full text Add to dashboard Cite

show abstract

“…Collectively this information from proteomic studies provides a better clinical picture of T1DM complications and provides a potential for screening patients. In a similar vein, proteomics technologies give a new insight into biological processes involved in several clinical problems associated with pregnancy, including pre-term birth, preeclampsia, and fetal growth alterations 11 .…”

Section: Introductionmentioning

confidence: 99%

Maternal serum proteomic profiles of pregnant women with type 1 diabetes

Gutaj

Matysiak

Matuszewska

et al. 2022

Sci Rep

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Despite improvement in the care of diabetes over the years, pregnancy complicated by type 1 diabetes (T1DM) is still associated with adverse maternal and neonatal outcomes. To date, proteomics studies have been conducted to identify T1DM biomarkers in non-pregnant women, however, no studies included T1DM pregnant women. In this study serum proteomic profiling was conducted in pregnant women with T1DM in the late third trimester. Serum samples were collected from 40 women with T1DM and 38 healthy controls within 3 days before delivery at term pregnancy. Significant differences between serum proteomic patterns were revealed, showing discriminative peaks for complement C3 and C4-A, kininogen-1, and fibrinogen alpha chain. Quantification of selected discriminative proteins by ELISA kits was also performed. The serum concentration of kininogen-1 was significantly lower in women with T1DM than in controls. There were no significant differences in serum concentrations of complement C3 and complement C4-A between study groups. These data indicate that pregnant women with T1DM have a distinct proteomic profile involving proteins in the coagulation and inflammatory pathways. However, their utility as biomarkers of pregnancy complications in women with T1DM warrants further investigation.

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“…These biomarkers can be useful in the prediction and early diagnosis of the disease and its complications, such as retinopathy and nephropathy in the non-pregnant state 9,10 . Proteomics technologies give a new insight into biological processes involved in several clinical problems associated with pregnancy, including preterm birth, preeclampsia, and fetal growth alterations 11 .…”

Section: Introductionmentioning

confidence: 99%

Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes

Gutaj

Matysiak

Matuszewska

et al. 2021

Preprint

View full text Add to dashboard Cite

Despite improvement in the care of diabetes over the years, pregnancy complicated by type 1 diabetes (T1DM) is still associated with adverse maternal and neonatal outcomes. To date, proteomics studies have been conducted to identify T1DM biomarkers in non-pregnant women, however, no studies included T1DM pregnant women. In this study serum proteomic profiling was conducted in pregnant women with T1DM in the late third trimester. Serum samples were collected from 40 diabetic women and 38 healthy controls within 3 days before delivery at term pregnancy. Significant differences between serum proteomic patterns were revealed, showing discriminative peaks for complement C3 and C4-A, kininogen-1, and fibrinogen alpha chain. Quantification of selected discriminative proteins by ELISA kits was also performed. The serum concentration of kininogen-1 was significantly lower in women with T1DM than in controls. There were no significant differences in serum concentrations of complement C3 and complement C4-A between study groups. These data indicate that pregnant women with T1DM have a distinct proteomic profile involving proteins in the coagulation and inflammatory pathways. However, their utility as biomarkers of pregnancy complications in women with T1DM warrants further investigation.

show abstract

The Use of New Technologies in the Study of Pregnancy Disorders: The OMICS Approach

Cited by 3 publications

References 31 publications

Epigenetic impact of long non-coding RNA lnc-ADAM9 on extracellular matrix pathway in preterm syndrome through down-regulation of mRNA-ADAM9

Epigenetic impact of long non-coding RNA lnc-ADAM9 on extracellular matrix pathway in preterm syndrome through down-regulation of mRNA-ADAM9

Maternal serum proteomic profiles of pregnant women with type 1 diabetes

Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes

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