The Use of Response Surface Methodology as a Statistical Tool for Media Optimization in Lipase Production from the Dairy Effluent Isolate <i>Fusarium solani</i>

Kanmani, Paulraj; Karthik, S.; Aravind, J.; Kumaresan, Kuppamuthu

doi:10.5402/2013/528708

Cited by 33 publications

(22 citation statements)

References 26 publications

(22 reference statements)

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“…The analysis indicated significant quadratic models for the respective responses (< 0.0001), and adequate to denoting the mathematical relationship between variables and to measuring the degree of variability in response variables accounted for by the experimental process variables and their specific interactions [39]. The quadratic models were acknowledged good and adequate for the prediction of the responses, owing to their high R 2 values, low p values and high F values, respectively [38,40]. It was reported that the magnitude of adjusted and predicted R 2 values can be applied as a measure of proximity of experimental and predicted values [40,41].…”

Section: Discussionmentioning

confidence: 83%

“…The quadratic models were acknowledged good and adequate for the prediction of the responses, owing to their high R 2 values, low p values and high F values, respectively [38,40]. It was reported that the magnitude of adjusted and predicted R 2 values can be applied as a measure of proximity of experimental and predicted values [40,41]. In the present study, the adjusted and predicted R 2 values for the respective responses were in close conformity, with indication of high correlation between the experimental and predicted data (Table 3).…”

Section: Discussionmentioning

confidence: 99%

“…2). Kanmani et al [40] and Ramanan et al [42] reported the numerical importance of adequate precision to determining which experimental variable generates larger signal in relation to noise during the experiment. It was then inferred that adequate precision of experimental responses greater than four was considered beneficial.…”

Section: Discussionmentioning

confidence: 99%

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Role of Anthocleista vogelii in serum antioxidant defence system in cadmium-induced oxidative stress in Wistar rats

Apiamu

Asagba

Tonukari

2019

Beni-Suef Univ J Basic Appl Sci

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Background: Cadmium (Cd) toxicity, which runs across the food chain, is chiefly regulated by in vivo antioxidant defence system or through antioxidant supplementation of biological systems predisposed to this environmental stressor. The present study was designed to examine the role of Anthocleista vogelii leaves in Cd-induced oxidative stress in the serum of Wistar rats through the application of response surface methodology (RSM) and biomonitoring of selective responses: malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione-s-transferase (GST) and peroxidase (POD) activities, respectively. The cold macerated plant leaves were subjected to fractionation process using methanol-hexane-chloroform (3:2:1 v/v) solvent system such that n-hexane fraction with ample antioxidant levels in terms of total phenolic content (TPC) and total flavonoid content (TFC) among others at p < 0.05 was selected for the study. The study employed central composite design (CCD) with twenty experimental "runs" of male Wistar rats for twenty-eight days, following a week of acclimatization, where n-hexane fraction of A. vogelii (NFAV), cadmium chloride (CdCl 2) and body weights of rats were considered input factors in the study. Results: The study generated five quadratic models, which differed significantly at p < 0.05 for MDA levels as well as CAT, SOD, GST and POD activities in the sera of Wistar rats. The study revealed that exposure to Cd toxicity caused a marked increase (p < 0.05) in serum MDA levels, but a significant inhibition (p < 0.05) of serum SOD, CAT, GST and POD activities. However, Cd interaction with NFAV showed marked amelioration of Cd-induced oxidative stress, which was confirmed by significant decrease in serum MDA levels, but significant increase in serum SOD, CAT, GST and POD activities at p < 0.05 via the response surface plots. The study also confirmed the reliability and adequacy of the models for accurate prediction of the responses since R-squared (R 2) values obtained were greater than 90%. Conclusion: It was inferred from the present study that the adequacy of the models validated the potency of A. vogelii leaves graphically in the amelioration of Cd-induced oxidative stress in the serum of Wistar rats. Hence, the plant was considered a rich source of bioactive compounds with significant antioxidant properties.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

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Role of Anthocleista vogelii in serum antioxidant defence system in cadmium-induced oxidative stress in Wistar rats

Apiamu

Asagba

Tonukari

2019

Beni-Suef Univ J Basic Appl Sci

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“…Statistical optimizations such as RSM are studies where participating parameters are varied simultaneously for optimization experiments (Greasham and Inamine, 1986; Greasham and Herber, 1997; Zhang et al, 2000; Margarita et al, 2005). RSM can collectively optimize all the affecting parameters to eliminate the limitations with single-factor optimization process (Gough et al, 1996; Dubey et al, 2011; Kanmani et al, 2013). We therefore, attempted to decipher and model glucose and NaCl content of the growth medium, using R. toruloides.…”

Section: Introductionmentioning

confidence: 99%

Concomitant Production of Lipids and Carotenoids in Rhodosporidium toruloides under Osmotic Stress Using Response Surface Methodology

et al. 2016

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As a replacement to existing fossil fuels, biofuels, have proven their worth; however, their widespread use is limited due to inconsistent yields, higher costs and poor productivity. An oleaginous yeast, Rhodosporidium toruloides has been reported to accumulate substantial amounts of lipids (that can be converted to biofuels) and therefore, it was selected for study and optimization. Apart from lipids, R. toruloides is also reported to produce carotene that can be used as a therapeutic agent. In this study, the culture medium was statistically modeled and optimized for concomitant production of lipids and carotenoids and for improving and maximizing the productivity of lipids as well as carotenes. The two metabolites were expressed differentially in the growth cycle of the organism. Culture medium components were simultaneously varied at five different levels using statistical modeling employing response surface methodology (RSM). Osmotic stress was introduced in order to simulate saline conditions and optimize the carotenoid as well as lipid production process, to be used in conditions with high salt contents. We observed a 10% (w/v) increase in carotenoid production in initial experiments under osmotic stress due to high salt concentration, while the increase in lipid synthesis was not pronounced. In this study, we demonstrate 36.2% (w/v) lipid production and 27.2% (w/v) carotenoid production, under osmotic stress with high salt concentrations, for the first time.

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“…This method is feasible and practical when only a limited number of variables are involved in the experiment, but when the variables involved are large then this method becomes laborious and necessitates a daunting number of trials compounded with a drawback as it fails to incorporate the interaction effects between the variables on the process (Kanmani et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Response surface methodology optimization of nickel (II) removal using pigeon pea pod biosorbent

Aravind¹,

Lenin²,

Nancyflavia³

et al. 2013

Int. J. Environ. Sci. Technol.

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Pod of pigeon pea (Cajanus cajan), a celluloserich agricultural residue, was investigated for its nickel binding efficiency. The influence of key physicochemical parameters such as contact time, initial metal ion concentration, adsorbent dosage and pH on nickel (II) removal was studied. The equilibrium time was found to be 45 min. The optimum Ni (II) removal was obtained at an initial metal ion concentration of 80 mg/l, pH of 9.0 and an adsorbent dose of 400 mg/100 ml. A search for optimal combination of key variables was studied by response surface methodology for maximum removal of nickel. The experiment encompassing 17 runs was established with the aid of Box-Behnken design. Owing to the reasonable agreement between predicted and adjusted R 2 value (0.9714), the corresponding quadratic model gives the most appropriate relationship between the variables and response. The optimal point obtained was located in the valid region and the optimum adsorption parameters were predicted as an initial Ni (II) concentration of 60 mg/l, pH value of 9.0 and contact time of 75 min. Under these adsorption conditions, a maximum removal of 96.54 % of initial metal concentration was demonstrated.

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The Use of Response Surface Methodology as a Statistical Tool for Media Optimization in Lipase Production from the Dairy Effluent Isolate Fusarium solani

Cited by 33 publications

References 26 publications

Role of Anthocleista vogelii in serum antioxidant defence system in cadmium-induced oxidative stress in Wistar rats

Role of Anthocleista vogelii in serum antioxidant defence system in cadmium-induced oxidative stress in Wistar rats

Concomitant Production of Lipids and Carotenoids in Rhodosporidium toruloides under Osmotic Stress Using Response Surface Methodology

Response surface methodology optimization of nickel (II) removal using pigeon pea pod biosorbent

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