The use of the intestinal epithelial cell culture model, Caco-2, in pharmaceutical development

Engineering Polysaccharide-Based Polymeric Micelles to Enhance Permeability of Cyclosporin A Across Caco-2 Cells

“…Cells were incubated for 21-25 days to allow confluency, full maturation, including P-gp expression (48) and increased transepithelial electrical resistance (TEER) due to the formation of tight junctions in the cell monolayer (64). The culture medium was replaced [1.5 ml apical (AP) side and 2.6 ml basolateral (BL) side] every other day for the first week and daily thereafter.…”

A review on the effects of prebiotics on cell toxicity and integrity

“…[43] To determine the integrity of tight junctions physiological changes, trans-epithelial electrical resistance (TEER) is determined using Epithelial Voltohmmeter (EVOM). [44] The TEER readings are taken after the cells had been seeded into Transwell® insert and are polarized after days of incubation. The Transwell® has inner and outer compartments that depict apical (lumen) and basolateral (extracellular fluids) layers in vivo.…”

Magnesia–zirconia based mimetic biomembrane chromatography for predicting human drug absorption

“…Drugs are absorbed through epithelial cell layers by diffusion across the lipid bilayer of the cell membranes by transport via membrane proteins (transcellular pathway), through the paracellular pathway, or by transcytosis. In order to predict oral drug absorption, several in vitro methods had been developed: QSAR models [2][3][4][5][6][7], Caco-2 cell monolayers [8][9][10][11][12][13][14][15], parallel artificial membrane permeation assay [16,17], immobilized artificial membrane (IAM) chromatography [18][19][20][21][22], immobilized liposome chromatography [23][24][25][26][27][28], micellar chromatography [29][30][31], biopartitioning micellar chromatography [32][33][34], surface plasmon resonance (SPR) biosensors [35].…”

“…Column, 50 mm × 4.6 mm; mobile phase, Tris-HCl buffer (pH 7.4) containing 0.05 M NaCl; flow rate, 1 ml/min; solutes: (1) mannitol, (2) acyclovir, (3) ranitidine, (4) atenolol, (5) cimetidine, (6) theophylline, (7) acetaminophen, (8) antipyrine, (9) cephalexin, (10) timolol,(12) ketoprofen,(13) piroxicam,(14) lidocaine, (16) metoprolol, (17) diazepam,(19) hydrocortisone,(20) phenytoin,(22) propanolol.…”