The Use of Vegetative Compatibility and Heterokaryosis to Determine Potential Asexual Gene Exchange in Colletotrichum gloeosporioides

Chacko, R. J.; Weidemann, G. J.; TeBeest, D. O.; Correll, James C.

doi:10.1006/bcon.1994.1048

Cited by 25 publications

(15 citation statements)

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“…The characterization of VCGs has been employed successfully in various species of Colletotrichum (Brooker et al, 1991;Skroch et al, 1992;Wasilwa et al, 1993;Correll et al, 1993;Chacko et al, 1994;Beynon et al, 1995;Gichuru et al, 2000;Nitzan et al, 2002;Varzea et al, 2002;Abang et al, 2004;Pereira, 2005), and nitrate non-utilizing mutants have been used to demonstrate heterokaryosis and parasexual segregation in C. lindemuthianum isolates (Castro-Prado et al, 2007). However, little could be inferred about vegetative compatibility in this species, because the nit mutants were obtained from only five isolates.…”

Section: Introductionmentioning

confidence: 99%

Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil

Barcelos¹,

Souza²,

Silva³

2011

Genet. Mol. Res.

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ABSTRACT. The causal agent of common bean anthracnose, Colletotrichum lindemuthianum, has considerable genetic and pathogenic variability, which makes the development of resistant cultivars difficult. We examined variability within and between Brazilian pathotypes of C. lindemuthianum through the identification of vegetative compatibility groups (VCGs) and by RAPD analysis. Two hundred and ninety-five nit mutants were obtained from 47 isolates of various pathotypes of the fungus collected from different regions, host cultivars and years. In complementation tests, 45 VCGs were identified. Eighteen RAPD primers were employed in the molecular analyses, producing 111 polymorphic bands. Estimates of genetic similarities, determined from the Sorence-Dice coefficient, ranged from 0.42 to 0.97; the dendrogram obtained by cluster analysis revealed 18 groups of isolates. RAPD and VCG markers presented high genotypic diversity. The number of significant associations (P = 0.05) between RAPD, VCG and pathogenicity markers ranged from 0 (VCG) to 80% (pathogenicity). The test of multilocus association (r d ) for RAPD markers was significantly different from zero (P < 0.001), suggesting linkage disequilibrium. However, the results for VCG markers show the presence of recombination mechanisms. In conclusion, RAPD markers and VCGs were useful for detecting genetic variability among isolates of C. lindemuthianum. We found considerable diversity among isolates from the same geographic origin within a short interval; this suggests rapid evolution. There is a need for further studies to elucidate the population structure of this pathogen in agro-ecosystems.

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Section: Introductionmentioning

confidence: 99%

Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil

Barcelos¹,

Souza²,

Silva³

2011

Genet. Mol. Res.

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“…In some species, the diploid phase is considered transient, usually not identified, and the recombinants can be obtained directly from the heterokaryon. This process was called parameiosis (Bonatelli Jr et al, 1983;Bagagli et al, 1991) and have been observed in many fungal species, including Fusarium oxysporum (Molnar et al, 1990), Metarhizium anisopliae (Bagagli et al, 1991;Kava-Cordeiro et al, 2005), Cladosporium fulvum (Arnau and Oliver, 1993), Colletotrichum gloesporioides (Chacko et al, 1994), Beauveria bassiana (Paccola-Meirelles and Azevedo, 1991;Bello and Paccola-Meirelles, 1998). The results presented here showed that the oval conidia were efficient in obtaining the heterokaryon in C. sublineolum, representing an additional tool for genetic studies, mainly in strains incapable of producing falcate conidia.…”

Section: Resultsmentioning

confidence: 99%

Use of oval conidia as a tool to assess the genetic transfer among Colletotrichum sublineolum mutants

Nunes

Souza-Paccola

Andrade-Nobrega

et al. 2010

Braz. arch. biol. technol.

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The aim of this study was to assess oval conidia production in mutants for the reductase nitrate gene in C. sublineolum and verify the possibility of using them as a tool to transfer the genetic material in this species. The mutants used in the present study lost the ability to form falcate conidia but all produced oval conidia. The number of nuclei by conidium was evaluated. Oval conidia were efficient in the heterokaryon formation and these heterokaryons in liquid culture medium they also produced oval conidia in abundance. Recombinants were obtained and the genetic exchanges were confirmed by the RAPD analyses.

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“…Traditionally, the heterokaryon status of C. gloeosporioides has been determined by the recovery of both parental types from mononucleate conidia (Chacko et al 1994). To test whether the AB hph/phleo cultures contained both nuclei of the A hph and B phleo parents, aliquots of approximately 200 conidia from five AB hph/phleo cultures were spread on PDA media with either hygromycin or phleomycin, as well as onto media with both antibiotics, and allowed to germinate.…”

Section: Heterokaryon Formation From a Hph And B Phleo Conidial Pairingsmentioning

confidence: 99%

Slow-growing heterokaryons as potential intermediates in supernumerary chromosome transfer between biotypes of Colletotrichum gloeosporioides

Manners

2011

Mycol Progress

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The vegetative transfer of a supernumerary chromosome between two biotypes (A and B) of Colletotrichum gloeosporioides pathogenic on Stylosanthes spp. has been previously demonstrated. The mechanism of transfer is unknown, and transient heterokaryosis has been implicated as an intermediary step, but inter-biotype heterokaryons have not so far been isolated. Conidia of a hygromycin-resistant strain of biotype A and a phleomycinresistant strain of biotype B were mixed, co-cultured and plated on media containing both antibiotics and extremely slow-growing colonies resistant to both antibiotics isolated using repeated hyphal tip sub-culturing. Mononucleate conidia derived from these colonies were unable to germinate on media containing both antibiotics, but were able to germinate on media containing only one antibiotic, with hygromycin-resistant colonies predominating, indicating that unbalanced heterokaryons had formed. The heterokaryons had highly impaired growth rates suggesting some incompatibility. DNA marker analysis confirmed their heterokaryon status. These results demonstrate that unfit inter-biotype heterokaryons can form and potentially provide an intermediate step for supernumerary chromosomal exchange.

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The Use of Vegetative Compatibility and Heterokaryosis to Determine Potential Asexual Gene Exchange in Colletotrichum gloeosporioides

Cited by 25 publications

References 0 publications

Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil

Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil

Use of oval conidia as a tool to assess the genetic transfer among Colletotrichum sublineolum mutants

Slow-growing heterokaryons as potential intermediates in supernumerary chromosome transfer between biotypes of Colletotrichum gloeosporioides

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