The Utility of Ovotransferrin and Ovotransferrin‐Derived Peptides as Possible Candidates in the Clinical Treatment of Cardiovascular Diseases

Chen, Shuang; Jiang, Hongmei; Peng, Hanhui; Wu, Xiaosong; Fang, Jun

doi:10.1155/2017/6504518

Cited by 18 publications

(18 citation statements)

References 47 publications

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“…In this study, the administration of IQW to AS model mice was found to delay progression of the disease, reduce the arthritic grade of intervertebral joints and reduce the concentrations of IL‐6, IL‐1β and TNF‐α. The expression of cytokine‐induced inflammatory protein in the vascular endothelium can be successfully downregulated by administration of IRW (Ile‐Arg‐Trp) and IQW derived from the egg‐white protein ovotransferrin (Majumder and Wu ; Chen et al ). These anti‐inflammatory effects are exerted at least partly by alteration of the NF‐κB pathway (Kaustav et al ) and have sometimes been accompanied by additional beneficial antioxidant effects (Huang et al ).…”

Section: Discussionmentioning

confidence: 99%

“…Naturally occurring peptides and proteins have shown significant promise for the prevention, management or treatment of AS, largely due to their versatility as starting materials for the formulation of novel compounds with advantageous biological effects (Orekhov ). In particular, cytokine‐induced inflammatory protein expression is effectively downregulated by Ile‐Gln‐Trp (IQW, obtained from egg white), at least partly via adjustment of the NF‐κB pathway (Kaustav et al ; Chen et al ). Moreover the positive results of such anti‐inflammatory functions are often enhanced by concomitant antioxidant effects (Huang et al ; Liu et al ).…”

Section: Introductionmentioning

confidence: 99%

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Modulation of inflammatory response and gut microbiota in ankylosing spondylitis mouse model by bioactive peptide IQW

Liu

Yang

et al. 2020

J Appl Microbiol

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Aims Ankylosing spondylitis (AS) is a widespread and chronic inflammatory autoimmune disease of unknown provenance. Naturally occurring peptides and proteins have shown significant promise as modulators of immune responses. Thus, the aims of this study were to assess the protective effects of the bioactive peptide IQW (Ile‐Gln‐Trp) with respect to inflammatory indicators, gut microbiota and oxidative stress, and to examine the potential mechanisms of these effects. Methods and Results A mouse model was prepared by four injections of human proteoglycan extract (2 mg) in dimethyldioctadecylammonium solution (2 mg) over an interval of 2 weeks. Enzyme‐linked immunosorbent assay results for the markers of oxidative stress and inflammation in the AS mice revealed increased concentrations of malondialdehyde, IL‐6, IL‐1β and TNF‐α, along with decreased concentrations of catalase (CAT), glutathione peroxidase (GSH‐PX) and superoxide dismutase (SOD). Treatment with IQW was found to decrease the concentrations of IL‐6, IL‐1β and TNF‐α, and increase the concentrations of CAT, GSH‐PX and SOD. Moreover the quantification of the microbiota via 16s rRNA sequencing revealed a reduced microbial diversity in the AS mice, while a significantly increased microbial diversity was displayed by those treated with IQW. Whereas, there was a significant reduction in the relative abundance of Bacteroidetes and an increased relative abundance of Verrucomicrobia in AS mice, this was reversed following the IQW treatment. Conclusions The results demonstrated that IQW exerts a beneficial influence in AS by delaying progression of the disease, reducing the arthritic grade of intervertebral joints, altering the concentrations of cytokines and modulating the microbial diversity and composition. Significance and Impact of the Study Oral IQW treatment might represent a new approach to mitigate the onset and development of AS.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modulation of inflammatory response and gut microbiota in ankylosing spondylitis mouse model by bioactive peptide IQW

Liu

Yang

et al. 2020

J Appl Microbiol

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“…Previous studies have also associated changes in dietary habits (particularly the adoption of a HFD) with gut microbiota dysbiosis, which suggests clear consequences in terms of metabolic disorders (Chen et al ; Yin et al ). Along with the above‐mentioned positive effects of dietary quercetin on metabolic and immune responses, this study also revealed the beneficial impacts of quercetin on dysbiosis in mice fed a HFD.…”

Section: Discussionmentioning

confidence: 99%

“…Increasing interest has been directed towards the search for natural substances that may assist with CVD treatment. Health-promoting adjustments in lifestyle and dietary habits, including cognitive-behavioural therapy, physical exercise and a personalized diet, represent the most cost-effective forms of CVD management (Tangney and Rasmussen 2013;Chen et al 2017;Ding et al 2018). Notably, several investigations have also identified the dietary intake of polyphenols as a potential option for CVD management (Tangney and Rasmussen 2013;Ding et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Quercetin reduces atherosclerotic lesions by altering the gut microbiota and reducing atherogenic lipid metabolites

et al. 2019

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Aims Epidemiological studies have correlated cardiovascular disease and atherosclerosis with lifestyle factors such as sedentary behaviour and a high‐calorie diet. Recent studies of pathogenesis have highlighted the significance of the intestinal microbiota and chronic inflammation with respect to both the onset and development of atherosclerosis. This study examined the hypothesis that the oral administration of quercetin to low‐density lipoprotein receptor‐null (Ldlr−/−) mice would improve gut health by altering the gut microbiota and controlling the levels of atherogenic lipid metabolites and proinflammatory mediators in the intestine and serum. Methods and Results Mice were maintained on a high‐fat diet with or without oral quercetin administration for 12 weeks. Quercetin treatment suppressed body weight gains and reduced the extent of atherosclerotic lesions in the aortic sinus. Reduced malondialdehyde and increased interleukin 6 levels further indicated the protective effect of quercetin against immune/inflammatory responses and oxidative stress. Furthermore, quercetin led to decreased intestinal levels of cholesterol, lysophosphatidic acids and atherogenic lysophosphatidylcholine (LPC 18:1) and an increased level of coprostanol. A phylum‐level microbial analysis revealed that quercetin treatment reduced the abundance of Verrocomicrobia and increased microbiome diversity and the abundances of Actinobacteria, Cyanobacteria and Firmicutes. A Spearman analysis revealed negative correlations of Actinobacteria with intestinal and plasma LPC 18:1 and caecal cholesterol levels and of Firmicutes and Cyanobacteria with the plasma LPC 18:1 level. Conclusions This study demonstrated the ability of quercetin treatment to reduce lipid levels, as well as the areas of atherosclerotic lesions and sizes of plaques. This treatment also altered the composition of the gut microbiota and decreased the levels of atherogenic lipid metabolites. Significance and Impact of the Study Oral quercetin treatment may represent a new approach to mitigating the onset and development of atherosclerosis.

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“…Studies have demonstrated the successful inhibition of cytokine-induced inflammatory protein expression in vascular endothelium, at least in part via the modulation of the NF-B pathway, in the presence of two novel peptides, namely IRW and IQW, which are derived from the egg white protein ovotransferrin [13][14][15]. In addition to this anti-inflammatory effect, the observed advantages of these peptides have included antioxidant effects [16].…”

Section: Introductionmentioning

confidence: 99%

Effects of IRW and IQW on Oxidative Stress and Gut Microbiota in Dextran Sodium Sulfate-Induced Colitis

Liu

Yan

Ding

et al. 2018

Cell Physiol Biochem

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Background/Aims: There are known links between inflammatory bowel disease (IBD) and changes in the microbiota of the gut and inflammation and oxidative stress. In this study, a colitis model induced by dextran sodium sulfate (DSS) in mice is used to evaluate whether the presence of bioactive peptides IRW (Ile-Arg-Trp) and IQW (Ile-Gln-Trp) peptides is advantageous. Methods: The mice were arbitrarily assigned to the following four groups: (i) control (untreated), (ii) dextran sodium sulfate (DSS) treated, (iii) IRW-DSS treated, and (iv) IQW-DSS treated. For 7 days, the control group subjects had unrestricted access to untreated drinking water, whereas the drinking water supplied to the subjects in the DSS, IRW-DSS, and IQW-DSS groups during this period consisted of 5% DSS solution. The colonic lesions were scored after hematoxylin and eosin staining. Serum antioxidant capacity was analyzed by 2,2’-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) radical cation decolorization test and the microbiota in the colonic contents were sequenced by HiSeq2500 PE250. Results: The presence of DSS reduced daily weight gain, enhanced histopathology scores, and inhibited antioxidant enzyme expression. Superoxide dismutase, catalase, and glutathione peroxidase activities in the DSS-induced colitis model were significantly enhanced (P < 0.05) in the presence of dietary IRW and IQW. Furthermore, the Simpson index was significantly increased (P < 0.05) in the presence of dietary IRW and IQW compared to the control group. IRW and IQW increased the abundance of Coprococcus_1, Ruminococcaceae_UCG-014, and Desulfovibrio compared to the control group and DSS group. Furthermore, IQW decreased the abundance of Bacteroides in relation to the control group, but increased Parabacteroides. In addition, IRW increased the level of Anaerotruncus, Oscillibacter, and Ruminiclostridium_9 compared to the control group. Conclusion: This study concludes that the presence of IRW or IQW can mitigate DSS-induced oxidative stress by improving the activities of antioxidant enzymes, increasing intestinal microbial diversity and enhancing the abundance of gut microbiota, which may help maintain the homeostasis of host health and microenvironment in a DSS-induced mouse model, thus providing a potential further treatment for IBD patients.

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The Utility of Ovotransferrin and Ovotransferrin‐Derived Peptides as Possible Candidates in the Clinical Treatment of Cardiovascular Diseases

Cited by 18 publications

References 47 publications

Modulation of inflammatory response and gut microbiota in ankylosing spondylitis mouse model by bioactive peptide IQW

Modulation of inflammatory response and gut microbiota in ankylosing spondylitis mouse model by bioactive peptide IQW

Quercetin reduces atherosclerotic lesions by altering the gut microbiota and reducing atherogenic lipid metabolites

Effects of IRW and IQW on Oxidative Stress and Gut Microbiota in Dextran Sodium Sulfate-Induced Colitis

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