The utility of toluidine blue staining and brush cytology as adjuncts in clinical examination of suspicious oral mucosal lesions

Güneri, Pelin; Epstein, Joel B.; Kaya, Ahmet Hilmi; Veral, Alı; Kazandi, Ali Can; Boyacıoğlu, Hayal

doi:10.1016/j.ijom.2010.10.022

Cited by 36 publications

(32 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results are in agreement with the findings of Epstein et al which show that toluidine blue retention test holds promise as a screening tool for high-risk oral precancerous lesions since it can reduce a large number of unnecessary biopsies [32]. Concurring with other studies [33,34], our results encourage consideration of TBLU as a viable and feasible screening method in high-prevalence and low-resource scenarios like India.…”

Section: Discussionsupporting

confidence: 92%

Evaluation of chemiluminescence, toluidine blue and histopathology for detection of high risk oral precancerous lesions: A cross-sectional study

Ujaoney

Motwani²,

Degwekar³

et al. 2012

BMC Clin Pathol

View full text Add to dashboard Cite

Background Early detection holds the key to an effective control of cancers in general and of oral cancers in particular. However, screening procedures for oral cancer are not straightforward due to procedural requirements as well as feasibility issues, especially in resource-limited countries. Methods We conducted a cross-sectional study to compare the performance of chemiluminescence, toluidine blue and histopathology for detection of high-risk precancerous oral lesions. We evaluated 99 lesions from 55 patients who underwent chemiluminescence and toluidine blue tests along with biopsy and histopathological examination. We studied inter-as well as intra-rater agreement in the histopathological evaluation and then using latent class modeling, we estimated the operating characteristics of these tests in the absence of a reference standard test. Results There was a weak inter-rater agreement (kappa < 0.15) as well as a weak intra-rater reproducibility (Pearson's r = 0.28, intra-class correlation rho = 0.03) in the histopathological evaluation of potentially high-risk precancerous lesions. When compared to histopathology, chemiluminescence and toluidine blue retention had a sensitivity of 1.00 and 0.59, respectively and a specificity of 0.01 and 0.79, respectively. However, latent class analysis indicated a low sensitivity (0.37) and high specificity (0.90) of histopathological evaluation. Toluidine blue had a near perfect high sensitivity and specificity for detection of high-risk lesions. Conclusion In our study, there was variability in the histopathological evaluation of oral precancerous lesions. Our results indicate that toluidine blue retention test may be better suited than chemiluminescence to detect high-risk oral precancerous lesions in a high-prevalence and low-resource setting like India.

show abstract

Section: Discussionsupporting

confidence: 92%

Evaluation of chemiluminescence, toluidine blue and histopathology for detection of high risk oral precancerous lesions: A cross-sectional study

Ujaoney

Motwani²,

Degwekar³

et al. 2012

BMC Clin Pathol

View full text Add to dashboard Cite

show abstract

“…17 Güneri et al designed a comparative study between TB and brush cytology in order to evaluate the diagnostic efficacy of the two tested methods. 18 Mild and moderate dysplasia were considered as negative. They found a concordance (TB/brush cytology) of 30% for benign lesions and 61% for malignant lesions and concluded that adjuncts identified 92% of carcinoma in situ and SCC, in contrast to clinical findings alone in which 62% of these lesions were identified.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of autofluorescence and toluidine blue in the differentiation of oral dysplastic and neoplastic lesions from non dysplastic and neoplastic lesions: a cross-sectional study

et al. 2014

View full text Add to dashboard Cite

Abstract. The objective was to compare toluidine blue (TB) and autofluorescence (AF) for the detection of oral dysplasia and squamous cell carcinoma (OSCC) in clinically suspicious lesions according to conventional examination. Fifty-six clinically suspicious lesions were subjected to AF and TB examination. Data were compared using two different scenarios: in the first, mild dysplasia was considered as positive, while in the second, it was considered as negative. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV), accuracy, and concordance were calculated. AF sensitivity and specificity were 70.0 and 57.7%, respectively, while TB showed a sensitivity of 80% and a specificity of 61.5%. The sensitivity increased in the second scenario in both AF (76.5%) and TB (88.2%). The specificity decreased in AF and TB, showing the same value (51.3%). PPV was higher in TB than in AF (70.6 versus 65.6%) and similarly for NPV (72.7 versus 62.5%). In the second scenario, TB PPV was 44.1% and NPV was 90.9%; AF PPV was 40.6% and NPV was 83.3%. TB showed greater accuracy than AF in the first scenario (62.5 versus 58.9%). AF and TB are both sensitive but not specific in OSCC and dysplasia diagnosis. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

“…6 The dentists must know the possibility of lesion changes to be carsinoma. The lesion as chronic ulcer, white or red color, and swelling on mucous membran must be confirmed by biopsy.…”

Section: Discussionmentioning

confidence: 99%

“…Most carsinoma is found in late condition which is in difficult phase to be cured. 6,7,9 The patient was done the brush biopsy by specialist of anatomic pathology. The biopsy result from ulcer scrapping did not show the malignancy cell, so it was diagnosed as the chronic traumatic ulcer.…”

Section: Discussionmentioning

confidence: 99%

The management of chronic traumatic ulcer in oral cavity

Apriasari

2012

Dent. J. (Majalah Kedokteran Gigi)

View full text Add to dashboard Cite

show abstract

The utility of toluidine blue staining and brush cytology as adjuncts in clinical examination of suspicious oral mucosal lesions

Cited by 36 publications

References 41 publications

Evaluation of chemiluminescence, toluidine blue and histopathology for detection of high risk oral precancerous lesions: A cross-sectional study

Evaluation of chemiluminescence, toluidine blue and histopathology for detection of high risk oral precancerous lesions: A cross-sectional study

Evaluation of autofluorescence and toluidine blue in the differentiation of oral dysplastic and neoplastic lesions from non dysplastic and neoplastic lesions: a cross-sectional study

The management of chronic traumatic ulcer in oral cavity

Contact Info

Product

Resources

About