The warm temperature acclimation protein (Wap65) has an important role in the inflammatory response of turbot (Scophthalmus maximus)

Díaz‐Rosales, Patricia; Pereiro, Patricia; Figueras, António; Novoa, Beatriz; Dios, S.

doi:10.1016/j.fsi.2014.04.012

Cited by 32 publications

(6 citation statements)

References 55 publications

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“…Similarly, warmtemperature acclimation-related 65 kDa protein (Wap65), which is involved in the scavenging of free heme (80), was increased in abundance by net handling and hypoxia stressors. Wap65 in fish is the homologue of mammalian hemopexin (81) and in most teleosts presents two proteoforms (82). In this study, two spots were also matched to this protein suggesting the presence of these two proteoforms.…”

Section: Discussionsupporting

confidence: 66%

Protein changes as robust signatures of fish chronic stress: a proteomics approach in fish welfare research

Magalhães

Schrama

Farinha

et al. 2020

Preprint

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Background: In order to have a greater understanding of fish welfare, sensitive technological tools, such as proteomics, may assist the aquaculture industry as it allows an unbiased approach for the discovery of potential biomarkers for stress monitoring. Stress is characterized by a cascade of physiological responses that end-up inducing further changes at the whole-animal level that might either increase fitness or impair welfare. Monitorization of this dynamic process, up till now relies on indicators that are only a snapshot of the stress level experienced. Within this scope, using G ilthead seabream ( Sparus aurata ) as model, three chronic stress conditions, namely overcrowding, handling and hypoxia, were employed to evaluate the potential of the fish protein-based adaptations as reliable signatures of chronic stress, in contrast with the commonly used hormonal and metabolic indicators. Results: A large spectrum of biological variation regarding cortisol and glucose levels was observed, which values rose higher in net handled fish. In this sense, a potential pattern of stressor-specificity was evidenced since the magnitude of response and tolerance varied markedly from a permanent (crowding) to a repetitive stressor (handling). Gel-based proteomics analysis of the plasma proteome also revealed that net handled fish had the highest number of differential proteins, compared to the other trials . M ass spectrometric analysis, followed by gene ontology enrichment and protein-protein interaction analyses, characterized those as humoral components of the innate immune system and key elements on the response to stimulus. Conclusions: Overall, this study represents the first screening of more reliable signatures of physiological adaptation to chronic stress in fish, allowing the future development of novel biomarker models to monitor fish welfare.

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Section: Discussionsupporting

confidence: 66%

Protein changes as robust signatures of fish chronic stress: a proteomics approach in fish welfare research

Magalhães

Schrama

Farinha

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…WAP65 has been associated with seasonal acclimation to warm temperature in several fishes (goldfish, Kikuchi, Yamashita, Watabe, & Aida, ; mumichog killifish, Picard & Schulte, ; sweetfish, Li & Chen, ). This protein has high affinity to heme, and its main function is to transport this co‐factor to the liver and sequester reactive oxygen species generated as a reaction to a particular stressor (Diaz‐Rosales, Pereiro, Figueras, Novoa, & Dios, ). The WAP65 gene was also upregulated in Transgenerational +3.0°C with regard to Step +3.0°C.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and molecular consequences of stepwise temperature increase across generations in a coral reef fish

et al. 2018

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Global warming will have far‐reaching consequences for marine species over coming decades, yet the magnitude of these effects may depend on the rate of warming across generations. Recent experiments show coral reef fishes can compensate the metabolic challenges of elevated temperature when warm conditions are maintained across generations. However, the effects of a gradual temperature increase across generations remain unknown. In the present study, we analysed metabolic and molecular traits in the damselfish Acanthochromis polyacanthus that were exposed to +1.5°C in the first generation and +3.0°C in the second (Step +3.0°C). This treatment of stepwise warming was compared to fish reared at current‐day temperatures (Control), second‐generation fish of control parents reared at +3.0°C (Developmental +3.0°C) and fish exposed to elevated temperatures for two generations (Transgenerational +1.5°C and Transgenerational +3.0°C). Hepatosomatic index, oxygen consumption and liver gene expression were compared in second‐generation fish of the multiple treatments. Hepatosomatic index increased in fish that developed at +3.0°C, regardless of the parental temperature. Routine oxygen consumption of Step +3.0°C fish was significantly higher than Control; however, their aerobic scope recovered to the same level as Control fish. Step +3.0°C fish exhibited significant upregulation of genes related to mitochondrial activity and energy production, which could be associated with their increased metabolic rates. These results indicate that restoration of aerobic scope is possible when fish experience gradual thermal increase across multiple generations, but the metabolic and molecular responses are different from fish reared at the same elevated thermal conditions in successive generations.

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“…Most teleost species have two WAP65 (Warm Acclimation Protein 65) genes that are similar to the mammalian Hx. Although differences in their physiological function remain unclear, both retain heme binding motifs (Machado et al, 2014;Diaz-Rosales et al, 2014). Only one of these could be found in the available transcriptomes and genomes of the Antarctic notothenioids (WAP65-2), suggesting that the other has been lost.…”

Section: Divergent Evolutionary Fate Of Hp and Hxmentioning

confidence: 99%

A tale of two genes: divergent evolutionary fate of haptoglobin and hemopexin in hemoglobinless antarctic icefishes

Bilyk

Zhuang

Murphy

et al. 2019

Journal of Experimental Biology

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The evolution of Antarctic notothenioid fishes in the isolated freezing Southern Ocean has led to remarkable trait gains and losses. One of the most extraordinary was the loss of the major oxygen carrier hemoglobin (Hb) in the icefishes (family Channichthyidae). Although the mechanisms of this loss and the resulting compensatory changes have been well studied, the impact of Hb loss on the network of genes that once supported its recycling and disposal has remained unexplored. Here, we report the functional fate and underlying molecular changes of two such key Hb-supporting proteins across the icefish familyhaptoglobin (Hp) and hemopexin (Hx), crucial in removing cytotoxic free Hb and heme, respectively. Hp plays a critical role in binding free Hb for intracellular recycling and absent its primary client, icefish Hp transcription is now vanishingly little, and translation into a functional protein is nearly silenced. Hp genotype degeneration has manifested in separate lineages of the icefish phylogeny with three distinct nonsense mutations and a deletion frame shift, as well as mutated polyadenylation signal sequences. Thus, Hb loss appears to have diminished selective constraint on Hp maintenance, resulting in its stochastic, co-evolutionary drift towards extinction. Hx binds free heme for iron recycling in hepatocytes. In contrast to Hp, Hx genotype integrity is preserved in the icefishes and transcription occurs at levels comparable to those in the red-blooded notothenioids. The persistence of Hx likely owes to continued selective pressure for its function from mitochondrial and non-Hb cellular hemoproteins.

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The warm temperature acclimation protein (Wap65) has an important role in the inflammatory response of turbot (Scophthalmus maximus)

Cited by 32 publications

References 55 publications

Protein changes as robust signatures of fish chronic stress: a proteomics approach in fish welfare research

Protein changes as robust signatures of fish chronic stress: a proteomics approach in fish welfare research

Phenotypic and molecular consequences of stepwise temperature increase across generations in a coral reef fish

A tale of two genes: divergent evolutionary fate of haptoglobin and hemopexin in hemoglobinless antarctic icefishes

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