The Wheat Peptidyl Prolyl<i>cis</i>-<i>trans-</i>Isomerase FKBP77 Is Heat Induced and Developmentally Regulated1

Kurek, Isaac; Aviezer, Keren; Erel, Noa; Herman, Eliot M.; Breiman, Adina

doi:10.1104/pp.119.2.693

Cited by 81 publications

(68 citation statements)

References 64 publications

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“…AU164106 was very similar to the rice lipid transfer protein b21 gene (Ltp b21) (BLASTN E-value = e-103) whose expression was reported in the mesocotyl and root (Vignols et al 1997). On the other hand, AU092028 and AU090552 were very similar to the gene for wheat peptidylprolyl isomerase FKBP77 (EC 5.2.1.8) (BLASTX E-value = 7e-35) displaying a heat shock-induced expression (Kurek et al 1999), and the gene for rice chitinase IIb (EC 3.2.1.14) (BLASTX E-value = 5e-72) identified in rice husks as a candidate for dormancy-related protein (Nakazaki and Ikehashi 1998), respectively. AU164106 showed a similar expression pattern to that of AGPase, suggesting the existence of functions related to starch accumulation or accumulating sink (Fig.…”

Section: Unclassified Genesmentioning

confidence: 76%

Microarray Analysis of Sink-Source Transition in Rice Leaf Sheaths

et al. 2005

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In rice (Oryza sativa L.) plants, the leaf sheaths of the upper leaves accumulate a large amount of starch before heading, and the accumulated starch is converted to sucrose and translocated to the panicles after heading. To analyze the regulation of sink-source transition, we carried out large-scale monitoring of gene expression by microarray analysis using 8987 rice expressed sequence tags (ESTs), and identified 102 developmentally regulated genes in the leaf sheaths of the first leaves below the flag leaves during the heading period. Identified genes included multiple genes related to starch biosynthesis, cell division and expansion, and photosynthesis. All of them showed early stage-preferential expression, probably reflecting the decrease of starch biosynthesis, end of elongation and decrease of photosynthesis in the leaf sheaths during the heading period, respectively. The expression patterns of the genes for starch biosynthesis enzymes and α-tubulin suggest that the leaf sheaths displayed both accumulating-and consuming-sink functions at the very early stage of the heading period. Northern blot analysis of the genes for starch degradation and sucrose biosynthesis enzymes (α-amylase3D and sucrose phosphate synthase) revealed that the induction of the genes did not occur when the starch amount began to decrease, suggesting that the mechanisms of starch degradation and sucrose re-synthesis in the leaf sheaths during the heading period were different from those in germinating seeds. Furthermore, we identified 18 developmentally regulated genes whose functions in the leaf sheaths are unknown. They included seven genes those were preferentially expressed in the bottom part of the leaf sheaths, implying that they were involved in functions related to the starch metabolism. The results obtained showed the effectiveness of the microarray technique to analyze complex and uncharacterized phenomena.

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Section: Unclassified Genesmentioning

confidence: 76%

Microarray Analysis of Sink-Source Transition in Rice Leaf Sheaths

et al. 2005

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“…In plants, these proteins are involved in flowering (Wang et al, 2010) and in controlling cell proliferation, since PPIase expression increases in the presence of cytokinin (Vittorioso et al, 1998). PPIases are induced by wounding, heat and salt stress (Vucich & Gasser, 1996;Kurek et al, 1999), and have been directly involved in membrane protein folding (i.e., chloroplast and mitochondria) (Breiman et al, 1992). Thus, the quick re-establishment of the normal condition after desiccation in P. columbina could be in part explained by both: i) the re-folding of structural proteins with important functions such as those involved in transcriptional regulation, and ii) by the removal of oxidized proteins.…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

Contreras–Porcia¹,

López-Cristoffanini²,

Lovazzano³

et al. 2013

lajar

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ABSTRACT. In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone. Keywords: Pyropia, desiccation stress, ESTs, seaweeds, transcriptomics, proteins. Expresión diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta)bajo hidratación y desecación natural RESUMEN. En zonas rocosas costeras, la desecación es gatillada por cambios diarios en los niveles de marea, y la evidencia experimental indica que la distribución de las algas en la zona intermareal está relacionada con su capacidad para tolerar la desecación. En este contexto, la presencia de Pyropia columbina en la zona alta del intermareal se explica por su excepcional tolerancia fisiológica a la desecación. Este estudio explora las vías metabólicas involucradas en la tolerancia a la desecación en P. columbina, a través de la caracterización de su transcriptoma bajo condiciones de hidratación contrastantes. Se obtuvo 1,410 ESTs provenientes de dos librerías de substracción de cDNA de frondas naturalmente hidratadas y desecadas. Los transcriptomas de ambas librerías contienen transcritos de diversas rutas metabólicas relacionadas a la tolerancia. Entre los transcritos expresados 15% están involucrados en la síntesis de proteínas, su procesamiento y degradación, 14,4% asociados a fotosíntesis y cloroplasto, 13,1% a respiración y función mitocondrial, 10,6% al metabolismo de la pared celular y 7,5% a la actividad antioxidante, proteínas chaperonas y factores de defensa (catalasa, tiorredoxina, proteínas de shock térmico, citocromo P450). En ambas librerías se de...

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“…At least two high-molecular weight FKBP members have been identified from wheat (wFKBP73 and wFKBP77), two from Arabidopsis (ROF1͞AtFKBP61 and PAS1͞AtFKBP71) and one from maize (mzFKBP66; refs. [15][16][17][18][19][20]. A cytosolic FKBP12 also has been characterized in Arabidopsis (21,22).…”

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confidence: 99%

A chloroplast FKBP interacts with and affects the accumulation of Rieske subunit of cytochrome bf complex

Gupta

Mould

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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Immunophilins are intracellular receptors of the immunosuppressants cyclosporin A, FK506, and rapamycin. Although all immunophilins possess peptidyl-prolyl isomerase activity and are identified from a wide range of organisms, little is known about their cellular functions. We report the characterization and functional analysis of an FK506 and rapamycin-binding protein (AtFKBP13) from Arabidopsis. The AtFKBP13 protein is synthesized as a precursor that is imported into chloroplasts and processed to the mature form located in the thylakoid lumen, as shown by chloroplast import assays and Western blot analysis. Experiments show that AtFKBP13 is translocated across the thylakoid membrane by the ⌬pH-dependent pathway. Yeast twohybrid screening identified Rieske FeS protein, a subunit of the cytochrome bf complex in the photosynthetic electron transport chain, as an interacting partner for AtFKBP13. Both yeast two-hybrid and in vitro protein-protein interaction assays showed that the precursor, but not the mature form, of AtFKBP13 interacted with Rieske protein, suggesting that interaction between the two proteins occurs along the import pathway. When AtFKBP13 expression was suppressed by RNA interference method, the level of Rieske protein was significantly increased in the transgenic plants.

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The Wheat Peptidyl Prolylcis-trans-Isomerase FKBP77 Is Heat Induced and Developmentally Regulated1

Cited by 81 publications

References 64 publications

Microarray Analysis of Sink-Source Transition in Rice Leaf Sheaths

Microarray Analysis of Sink-Source Transition in Rice Leaf Sheaths

Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

A chloroplast FKBP interacts with and affects the accumulation of Rieske subunit of cytochrome bf complex

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