The pandemic COVID -19 causing the teaching style in higher education to change. This research aims to This study aims to investigate learners ' Digital Literacy (DL) knowledge in an applied computer course and how to improve assessment for learning (AfL). The sampling in research was 74 sample, student of studying Bachelor degree of Science in Food Technology. Mahidol University Kanchanauri Campus. Register course: Computer program applications. Statistics used for this research, frequency, mean, standard deviation, t-test, one-way ANOVA, two-way ANOVA and multiple regression analysis were used to analyse the data. which was the general information such as gender, the Operating System (OS) Dependent Variable, which was DL level (Access, Analyse, Create and Evaluate). The result show that multiple regression analysis with squared multiple correlations (R2) = 90.8%. It can be said that when the data of all 4 variables (creating, evaluating, analyse, accessing) were used to predict the learners ' learning level in relation to DL.