Therapeutic angiogenesis by a myoblast layer harvested by tissue transfer printing from cell-adhesive, thermosensitive hydrogels

Kim, Dong Wan; Jun, Indong; Lee, Tae Jin; Lee, Ji hye; Jang, Hyeon-Ki; Kang, Seokyung; Парк, Ки Донг; Cho, Seung Woo; Kim, Byung Soo; Shin, Heungsoo

doi:10.1016/j.biomaterials.2013.07.071

Cited by 17 publications

(11 citation statements)

References 44 publications

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“…Kim et al . () adopted a cell sheet‐engineering approach for the reconstruction of ischaemia‐damaged myocardium; they cultured C2C12 myoblasts on thermosensitive tetronic–tyramine hydrogel surfaces, incorporating cell adhesive GRGDGGGGGY peptides. After 1 day of culture, confluent sheet‐like myoblast monolayers were obtained by decreasing the temperature to 4°C.…”

Section: Potential Biomedical Applicationsmentioning

confidence: 99%

Horseradish peroxidase-catalysedin situ-forming hydrogels for tissue-engineering applications

Bae

Choi

Lee

et al. 2014

J Tissue Eng Regen Med

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117

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In situ-forming hydrogels are an attractive class of implantable biomaterials that are used for biomedical applications. These injectable hydrogels are versatile and provide a convenient platform for delivering cells and drugs via minimally invasive surgery. Although several crosslinking methods for preparing in situ forming hydrogels have been developed over the past two decades, most hydrogels are not sufficiently versatile for use in a wide variety of tissue-engineering applications. In recent years, enzyme-catalysed crosslinking approaches have been emerged as a new approach for developing in situ-forming hydrogels. In particular, the horseradish peroxidase (HRP)-catalysed crosslinking approach has received increasing interest, due to its highly improved and tunable capacity to obtain hydrogels with desirable properties. The HRP-catalysed crosslinking reaction immediately occurs upon mixing phenol-rich polymers with HRP and hydrogen peroxide (H2O2) in aqueous media. Based on this unique gel-forming feature, recent studies have shown that various properties of formed hydrogels, such as gelation time, stiffness and degradation rate, can be easily manipulated by varying the concentrations of HRP and H2O2. In this review, we outline the versatile properties of HRP-catalysed in situ-forming hydrogels, with a brief introduction to the crosslinking mechanisms involved. In addition, the recent biomedical applications of HRP-catalysed in situ-forming hydrogels for tissue regeneration are described.

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Section: Potential Biomedical Applicationsmentioning

confidence: 99%

Horseradish peroxidase-catalysedin situ-forming hydrogels for tissue-engineering applications

Bae

Choi

Lee

et al. 2014

J Tissue Eng Regen Med

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117

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“…The localization of highly differentiated C2C12 myoblasts in the implanted cell-sheet-graphene hybrid enhances vascularization, regeneration of skeletal muscles, and functional recovery. [ 31,62 ] Furthermore, the fl uorescence images indicate that C2C12 myoblasts on the surface of graphene electrodes suppress the recruitment of CD68-positive macrophages at the implanted site (Figure 7 f). Without the cell sheet, the device can cause acute immune responses.…”

Section: In Vivo Therapeutic Application Of the Cell Sheetmentioning

confidence: 95%

“…[ 34 ] All materials are stable and biocompatible in vivo according to previous reports. [31][32][33][34] Chronic immune responses in the long-term implantation study should be observed in the future. Serpentine-shaped, mesh-patterned graphene electrodes (≈50 µm width; Figure S1a, Supporting Information) are used to improve stretchability and softness of the system.…”

Section: Introductionmentioning

confidence: 99%

“…[31][32][33][34] The device is composed of a cell-sheet-graphene hybrid, an aligned C2C12 myoblast sheet (≈10 µm) [ 31 ] integrated on buckled, Au-doped, and mesh-patterned graphene electrodes (≈5 nm), [ 32 ] which is mounted on a submicronthick polyimide (PI) membrane (≈600 nm) [ 33 ] and a soft polydimethylsiloxane (PDMS) substrate (500 µm) ( Figure 1 a). [ 34 ] All materials are stable and biocompatible in vivo according to previous reports.…”

Section: Introductionmentioning

confidence: 99%

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Stretchable and Transparent Biointerface Using Cell‐Sheet–Graphene Hybrid for Electrophysiology and Therapy of Skeletal Muscle

Kim

Cho

et al. 2016

Adv Funct Materials

131

125

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Implantable electronic devices for recording electrophysiological signals and for stimulating muscles and nerves have been widely used throughout clinical medicine. Mechanical mismatch between conventional rigid biomedical devices and soft curvilinear tissues, however, has frequently resulted in a low signal to noise ratio and/or mechanical fatigue and scarring. Multifunctionality ranging from various sensing modalities to therapeutic functions is another important goal for implantable biomedical devices. Here, a stretchable and transparent medical device using a cell‐sheet–graphene hybrid is reported, which can be implanted to form a high quality biotic/abiotic interface. The hybrid is composed of a sheet of C2C12 myoblasts on buckled, mesh‐patterned graphene electrodes. The graphene electrodes monitor and actuate the C2C12 myoblasts in vitro, serving as a smart cell culture substrate that controls their aligned proliferation and differentiation. This stretchable and transparent cell‐sheet–graphene hybrid can be transplanted onto the target muscle tissue, to record electromyographical signals, and stimulate implanted sites electrically and/or optically in vivo. Additional cellular therapeutic effect of the cell‐sheet–graphene hybrid is obtained by integrated myobalst cell sheets. Any immune responses within implanted muscle tissues are not observed. This multifunctional device provides many new opportunities in the emerging field of soft bioelectronics.

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“…In this pilot study, a dose-dependent positive correlation was seen with limb reperfusion and the cell mass injected into the adductor muscle of a PAD mouse model, but especially pronounced in diabetic mice. Innovative approaches based on tissue transferprinting [120] of myoblast monolayers as well as three-dimensional aggregates of human umbilical vein endothelial cells and cord-blood mesenchymal stem cells [121] have demonstrated promise towards hastening tube formation.…”

Section: Future Directions and Considerationsmentioning

confidence: 99%

Engineering Integrative Stem Cell and Biomaterial Therapies for Peripheral Artery Disease

Balikov

Lee

Boire

et al. 2015

Biosystems &Amp; Biorobotics

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Because of their potential to regenerate tissues and organs, stem cells garner extensive interest worldwide for treating a wide range of degenerative diseases. In numerous efforts, a variety of stem cell formats have been considered for therapeutic purposes to combat such pathologies, one being vascular-related diseases. In particular, the prevalence of peripheral artery disease (PAD) has steadily increased with the growth of the aging population in many first-world countries. Considering this disturbing trend as well as the obesity epidemic and the ballooning population growth in third-world nations, the burden of PAD is expected to increase worldwide to alarming levels in the coming decades. The advent of stem cell treatments could stymie this burden by alleviating the complications and improving upon the less-than-satisfactory outcomes from current standard-of-care surgical/pharmacological interventions for PAD. This chapter reviews the relevant, cutting-edge clinical and animal model research efforts in the field and explores the remaining questions as they pertain to convergence technologies that may provide the potential for stem cells to reverse PAD-induced tissue damage. Harnessing and translating this potential to create more viable and efficacious PAD treatments should be of paramount global and public health concern.

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Therapeutic angiogenesis by a myoblast layer harvested by tissue transfer printing from cell-adhesive, thermosensitive hydrogels

Cited by 17 publications

References 44 publications

Horseradish peroxidase-catalysedin situ-forming hydrogels for tissue-engineering applications

Horseradish peroxidase-catalysedin situ-forming hydrogels for tissue-engineering applications

Stretchable and Transparent Biointerface Using Cell‐Sheet–Graphene Hybrid for Electrophysiology and Therapy of Skeletal Muscle

Engineering Integrative Stem Cell and Biomaterial Therapies for Peripheral Artery Disease

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