Therapeutic Effect of Hyperoxygenated Solution on Acute Lung Injury Induced by Oleic Acid

Abstract

Background/Aims: Hyperoxygenated solution (HOS) has been shown to protect the myocardium, spinal cord and brain from ischemic injury. In this study, we evaluated the effect of HOS on acute lung injury (ALI) induced by oleic acid in rabbits. Methods: 24 rabbits were randomized into four groups: control (C), oleic acid (OA), inhaled oxygen (OX), and HOS treatment (HOS). The ALI model was produced by administrating oleic acid intravenously. Half an hour after oleic acid infusion, animals received inhaled oxygen at 30% FiO₂ or 20 ml/kg HOS intravenously. Various parameters were measured during the 2 h after oleic acid treatment. Results: After treatment with oleic acid, mean arterial pressure and PaO₂ decreased significantly compared to group C (p < 0.01), while lung/body ratio, lung water content, and the levels of myeloperoxidase (MPO) and TNF-α in the serum and BALF increased significantly (p < 0.01). Histologically, interstitial edema, alveoli exudation and massive inflammatory cell infiltration were observed in the lung. However, when treated with 20 ml/kg HOS, PaO₂ significantly increased compared to group OA (p < 0.05). The MPO and TNF-α levels in the serum and BALF were decreased (p < 0.01), pulmonary edema was reduced (p < 0.01). Improved pathological manifestations were observed. Conclusion: HOS at 20 ml/kg has therapeutic effects to ameliorate the biological and morphological changes to the lung induced by oleic acid. HOS is a safe, simple and effective measure to protect animals from ALI.

Hyperoxygenated Solution: Effects on Acute Hypobaric Hypoxia-Induced Oxidative Damage in Rabbits

“…This may explain why the Pao 2 levels in the HOS group were higher than those in the HI group in the present study, although the use of an anesthetic induced a decrease in ventilatory responses in both experimental groups. Administration of HOS has been shown to preserve organ function and integrity after injury of the myocardium, spinal cord, brain, intestine, and lung in experimental animals ( Jia et al, 2000;Sun et al, 2004;Gao et al, 2006;Xu et al, 2008). In addition to its high O 2 dissolving capacity, HOS possesses other characteristics that may play key roles in alleviating tissue injury.…”

“…Photochemistry techniques can be used to transform commonly used medical solutions (such as 0.9% normal saline and 5% glucose solution) into HOS, the oxygen partial pressure (Po 2 ) of which can increase from 21 kPa (157.5 mmHg) to 106 AE 7.8 kPa (795 AE 58.5 mmHg) (Gao et al, 2006;Xu et al, 2004). Administration of HOS in animal models and patients has been shown to preserve organ function and integrity during ischemic injury and anoxic damage ( Jia et al, 2000;Sun et al, 2004;Gao et al, 2006;Xu et al, 2008). In the present study we used a rabbit simulated hypobaric hypoxia model to explore the applicability of HOS as a novel method for oxygen delivery compared with conventional therapies.…”

Propofol Activation of the Nrf2 Pathway Is Associated with Amelioration of Acute Lung Injury in a Rat Liver Transplantation Model

“…The water content of the lung was calculated: water content = (lung wet weight − lung dry weight)/lung wet weight × 100 [32]. …”

Potential Use of Hyperoxygenated Solution as a Treatment Strategy for Carbon Monoxide Poisoning

“…In addition, non-cardiogenic pulmonary edema caused by CO poisoning has another important role in the process of tissue hypoxia [25-27]. Our previous study indicated that in addition to hemodynamic amelioration, HOS could improve pulmonary permeability in the lungs of patients and decrease the likelihood of pulmonary edema [17]. Altogether, HOS could effectively relieve hypoxia, which could ultimately prevent or reduce the late cognitive sequelae.…”

“…The oxygen partial pressure in these solutions can reach 100-120 kPa, which is ten times higher than that in arterial blood [16]. Some animal experiments have proven that the administration of an HOS can partly attenuate the injuries caused by ischemia and hypoxia [17,18]. HOSs are expected to potentially serve as a novel and effective treatment for acute CO poisoning in human patients.…”