Therapeutic effect of vitamin B3 on hyperglycemia, oxidative stress and DNA damage in alloxan induced diabetic rat model

Abdullah, K.M.; Alam, Maroof; Iqbal, Zarmin; Naseem, Imrana

doi:10.1016/j.biopha.2018.06.085

Cited by 35 publications

(24 citation statements)

References 40 publications

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“…Different approaches have been used to develop DNP animal models. For example, DNP models can be established spontaneously, e.g., the WBN/Kob rat [46] and Ins2 Akita mouse [47], genetically, e.g., the C57BL/Ks (db/db) mouse model [48], through high-fat diets [49], or pharmacologically with alloxan [50] or STZ [51]. Because STZ triggers diabetes features that closely recapitulate those of human diabetes, it is extensively used to generate diabetes models [52].…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture alleviates diabetic neuropathic pain in rats by suppressing P2X3 receptor expression in dorsal root ganglia

Fei

Tai

et al. 2020

Purinergic Signalling

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Diabetic neuropathic pain (DNP) is a troublesome diabetes complication all over the world. P2X3 receptor (P2X3R), a purinergic receptor from dorsal root ganglion (DRG), has important roles in neuropathic pain pathology and nociceptive sensations. Here, we investigated the involvement of DRG P2X3R and the effect of 2 Hz electroacupuncture (EA) on DNP. We monitored the rats’ body weight, fasting blood glucose level, paw withdrawal thresholds, and paw withdrawal latency, and evaluated P2X3R expression in DRG. We found that P2X3R expression is upregulated on DNP, while 2 Hz EA is analgesic against DNP and suppresses P2X3R expression in DRG. To evaluate P2X3R involvement in pain modulation, we then treated the animals with A317491, a P2X3R specific antagonist, or α β-me ATP, a P2X3R agonist. We found that A317491 alleviates hyperalgesia, while α β-me ATP blocks EA’s analgesic effects. Our findings indicated that 2 Hz EA alleviates DNP, possibly by suppressing P2X3R upregulation in DRG.

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Section: Discussionmentioning

confidence: 99%

Electroacupuncture alleviates diabetic neuropathic pain in rats by suppressing P2X3 receptor expression in dorsal root ganglia

Fei

Tai

et al. 2020

Purinergic Signalling

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“…Under alkaline conditions the comet assay was performed in accordance with the protocol of Singh et al 30 A detailed protocol for the assay has been described in our previous publication by K. M. Abdullah et al 40 Scoring of the comet slides was performed using an image analysis system (Komet 5.5, Kinetic Imaging, Liverpool, UK) attached to an Olympus (CX41) fluorescence microscope (Olympus Optical Co, Tokyo, Japan).…”

Section: Comet Assay (Single Cell Alkaline Gel Electrophoresis)mentioning

confidence: 99%

Anti-diabetic study of vitamin B6 on hyperglycaemia induced protein carbonylation, DNA damage and ROS production in alloxan induced diabetic rats

et al. 2019

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“…Blood lipid disorders (HDL-C, LDL-C, cholesterol, and triglycerides) often lead to insulin resistance [2]. Compared to the control group, the serum levels of HDL-C were significantly lower in T2DM mice, which were restored by DhHP-6 at concentrations of 20 and 60 mg/kg bodyweight (Figure 8A).…”

Section: Resultsmentioning

confidence: 99%

“…Excessive ROS can induce oxidative damage to organs associated with metabolic process, like the liver and kidney. Moreover, T2DM can lead to liver dysfunction, such as fatty liver, liver fibrosis, and non-alcoholic fatty liver disease (NAFLD) [2,34,35]. In line with these studies, hepatic lobules were obsolete and the permutation of liver cells caused them to be irregular and swollen in T2DM mice.…”

Section: Discussionmentioning

confidence: 98%

“…Type 2 diabetes mellitus (T2DM) has increased in prevalence and emerged as one of the largest public health problems globally. According to the World Health Organization (WHO) report on diabetes, the number of people suffering from diabetes rose from 108 million to 422 million from 1980 to 2014, respectively [2]. The global prevalence of diabetes in adults according to the International Diabetes Federation (IDF) report published in 2013 was 8.3%, and the global prevalence is expected to rise to 10.1% [3].…”

Section: Introductionmentioning

confidence: 99%

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Oral DhHP-6 for the Treatment of Type 2 Diabetes Mellitus

Wang

Liang

et al. 2019

IJMS

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Type 2 diabetes mellitus (T2DM) is associated with pancreatic β-cell dysfunction which can be induced by oxidative stress. Deuterohemin-βAla-His-Thr-Val-Glu-Lys (DhHP-6) is a microperoxidase mimetic that can scavenge reactive oxygen species (ROS) in vivo. In our previous studies, we demonstrated an increased stability of linear peptides upon their covalent attachment to porphyrins. In this study, we assessed the utility of DhHP-6 as an oral anti-diabetic drug in vitro and in vivo. DhHP-6 showed high resistance to proteolytic degradation in vitro and in vivo. The degraded DhHP-6 product in gastrointestinal (GI) fluid retained the enzymatic activity of DhHP-6, but displayed a higher permeability coefficient. DhHP-6 protected against the cell damage induced by H2O2 and promoted insulin secretion in INS-1 cells. In the T2DM model, DhHP-6 reduced blood glucose levels and facilitated the recovery of blood lipid disorders. DhHP-6 also mitigated both insulin resistance and glucose tolerance. Most importantly, DhHP-6 promoted the recovery of damaged pancreas islets. These findings suggest that DhHP-6 in physiological environments has high stability against enzymatic degradation and maintains enzymatic activity. As DhHP-6 lowered the fasting blood glucose levels of T2DM mice, it thus represents a promising candidate for oral administration and clinical therapy.

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Therapeutic effect of vitamin B3 on hyperglycemia, oxidative stress and DNA damage in alloxan induced diabetic rat model

Cited by 35 publications

References 40 publications

Electroacupuncture alleviates diabetic neuropathic pain in rats by suppressing P2X3 receptor expression in dorsal root ganglia

Electroacupuncture alleviates diabetic neuropathic pain in rats by suppressing P2X3 receptor expression in dorsal root ganglia

Anti-diabetic study of vitamin B6 on hyperglycaemia induced protein carbonylation, DNA damage and ROS production in alloxan induced diabetic rats

Oral DhHP-6 for the Treatment of Type 2 Diabetes Mellitus

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