Therapeutic efficacy of Genistein-Cytoreg® combination in breast cancer cells

Abstract

Background: In spite the heavy investments in therapeutic research breast cancer still impacts the lives of women globally. The projected incidence of new cases of in situ breast cancer in the USA for 2011 is 57,650, with estimated 39,520 deaths. The phytoestrogen, genistein and the synthetic compound, Cytoreg® have been shown to inhibit growth and proliferation in many cancer cell lines. Purpose of the Study: In this study, we investigated the therapeutic efficacy of Cytoreg®-genistein combination on growth inhibition in the MCF-7 human breast cancer cells. Method: MCF-7 cells were treated with genistein and Cytoreg® single and combination treatments for 24-48hrs; and post treatment chemosensitivity assessed, using: Trypan Blue exclusion and MTT assays for cell viability, Ethidium bromide/Acridine orange to assess apoptosis induction, and FAM Poly-Caspase binding assay for mechanism of action. Results: The overall data indicated dose- and time-dependent cell death in the MCF-cells and apoptosis as the major means of treatment-induced growth inhibition with all the treatment regimens. Conclusion: Comparatively, the genistein-Cytoreg® combination treatment was significantly more efficacious in growth inhibition in the MCF cells than either genistein or Cytoreg® alone. Genistein seems to act additively with Cytoreg® in combination treatment-induced apoptosis in MCF-7 cells. The normal human breast epithelial cells were not significantly inhibited by either single or the combination treatments.Key words: Cytoreg®, Genistein, Combination treatment, MCF- cancer cells, apoptosis

Effect of an ionic antineoplastic agent Cytoreg on blood chemistry in a Wistar rat model

“…One of the reported therapeutic effects of Cytoreg is the inhibition of the growth and proliferation of many cancer cell lines. 36 Our findings indicate that Cytoreg has an important effect on cellular metabolism by increasing mitochondrial activity, and suggest a mechanism for cancer cell apoptosis through the generation of reactive oxygen species, which may be considered a therapeutic mechanism of action that alters cellular metabolism from a hypoxic, oxygen-deficient state with an initial pO 2 of 84.6 ± 7.97 mmHg to a totally aerobic environment 147.3 ± 10.1 mmHg 60 minutes after administration. This mechanism of action not only influences the growth of neoplastic tissue but also affects the aforementioned illnesses, which are related to proper mitochondrial function.…”

Evaluation of dermal corrosion and irritation by Cytoreg in rabbits

“…Cytoreg is an experimental therapeutic technology platform consisting of an aqueous solution of hydrofluoric, hydrochloric, sulfuric, phosphoric, citric, and oxalic acids [ 1 ]. In vitro and in vivo studies have shown Cytoreg to have oncolytic activity [ [2] , [3] , [4] , [5] ]. In Wistar rats, oral administration of Cytoreg is well tolerated [ 6 ] and was recently shown to increase arterial blood oxygen pressure (pO 2 ) by iv administration [ 7 ].…”