Therapeutic isoniazid monitoring using a simple high-performance liquid chromatographic method with ultraviolet detection

Ethyl Chloroformate as a Derivatizing Reagent for the Gas Chromatographic Determination of Isoniazid and Hydrazine in Pharmaceutical Preparations

“…For 10-fold addition of glucose, magnesium stearate, gum acacia, talcum, methylparaben, lactose and starch to INH did not affect its determination. Now, comparing the results with the reported HPLC method with UV detection, 32 which indicated a linear calibration range within 0.5 -8.0 mg/ml INH with a 50-ml loop injector with an analyses time of 15 min, as compared to the present method with linear calibration of 3.5 -37.5 mg/ml INH with 1 ml injection with a split ratio of 10:1, v/v and an analyses time of 11 min. The GC procedure is also free from the problem of safe disposal the used mobile phase.…”

“…10 Hydrazine (HZ) is a toxic substance and may be present in INH-based pharmaceuticals preparations as one of the decomposition products. 11 Various analytical techniques, such as titrimetry, 12,13 spectrophotometry, [14][15][16] spectrofluorometry, 17 atomic absorption, 18 chemiluminescence, 19 electroanalytical techniques, 20,21 kinetic determination, 22 flow injection, 23,24 thin layer chromatography, 25,26 capillary electrophoresis 27,28 and liquid chromatography [29][30][31][32] have been used for the quantitation of INH in pharmaceutical preparations and biological samples. Liquid chromatography with UV detection is carried out either by measuring the perse absorbance of INH at 263 nm or by precolumn derivatization with a suitable derivatizing reagent.…”

Simultaneous determination of isoniazid and pyrazinamide in plasma by high performance liquid chromatography

“…Several HPLC methods for the determination of one or more of the antituberculosis drugs in plasma or serum are available [8][9][10][11][12][13]. Some of these methods are developed for determining INH alone or with its major metabolites [8][9][10][11]; as a result, these methods cannot be applied for determination of PZA.…”

Simultaneous Determination of Isoniazid, Pyrazinamide, Rifampicin and Acetylisoniazid in Human Plasma by High-Performance Liquid Chromatography

“…However, these methods could not be applied to the determination of drugs in plasma or urine because of the complexity of these samples. Some HPLC methods [14][15][16] were developed to analyze the drugs in biological samples. However, these methods analyzed only one or two drugs in plasma, 12,[14][15][16] in urine 10 or in cells, 17 or have the limitation of complicated derivatization of the samples.…”

“…Some HPLC methods [14][15][16] were developed to analyze the drugs in biological samples. However, these methods analyzed only one or two drugs in plasma, 12,[14][15][16] in urine 10 or in cells, 17 or have the limitation of complicated derivatization of the samples. 14 Unsalan 18 had reported an LC method for the simultaneous determinations of PZA, INH and RFP within 11 min using a 150 mm long C18 column.…”