Thermal assembly of a biomimetic mineral/collagen composite

Advances in Collagen/Hydroxyapatite Composite Materials

“…Trying to obtain such composite with same composition and structure with the natural bone, the researchers elaborated many synthesis methods (Wahl and Czernuszka 2006) such as: in vitro collagen mineralization (Lawson and Czernuszka 1998), thermally -triggered assembly of hydroxyapatite/collagen gels (Pederson et al 2003), vacuum infiltration of collagen into a ceramic matrix (Werner et al 2002), enzymatic mineralization of collagen sheets (Yamauchi et al 2004), freeze drying and supercritical point drying (Pompe et al 2001), biomimetic synthesis (Li and Chang 2008). One of the most promising bone graft material seems to be the collagen/nanohydroxyapatite composite (Li et al 2006) due to its very good compositional and structural similarity with natural bone (Yunoki et al 2007;Yunoki et al 2006).…”

Biomimic Enamel Remineralization by Hybridization Calcium- and Phosphate-Loaded Liposomes with Amelogenin-Inspired Peptide

“…Release of entrapped ions at this temperature was due to increased permeability of phospholipid bilayers at the lipid chain melting temperature. Messersmith et al have proved the diffusion of Ca 2+ and PO 4 3-out of liposomes and their reaction to form crystalline calcium phosphate minerals, which were deposited on dentin and enamel surfaces or formation of mineral/collagen composite [11,12]. In the present study, we integrated the liposomes and the self-assembly amelogenin-inspired peptide to for enamel remineralization.…”

“…Calcium-and phosphate-loaded liposomes preparation Calcium-and phosphate-loaded liposomes were prepared using the interdigitation-fusion (IF) method as reported by Messersmith with some modification [10,11]. Briefly, 1,2-bis(palmitoyl)-sn-glycero-3-phosphocholine (DPPC > 99%) and 1,2-bis(myristoyl)-sn-glycero-3-phosphocholine (DMPC> 99%) (Sigma-Aldrich Trading Co., Ltd, American)were prepared at the mass ratio of 9:1, and hydrated with aqueous CaCl 2 or Na 2 HPO 4 .…”

“…Messersmith have developed a temperature-sensitive liposomes system to mimetic matrix vesicle to control mineralization study [10,11]. The liposomes loaded calcium and phosphate salts, and were stable at room temperature, but slowly released calcium and phosphate ions when heated to 37…”

The Use of Micro- and Nanospheres as Functional Components for Bone Tissue Regeneration

“…Fourth, micro-/nanospheres can be used as microscopic bioreactors to induce formation of apatite crystals and subsequent mineralization of hydrogels by releasing the resultant minerals, thus forming self-hardening biomaterials for bone regeneration. 19 This approach is inspired by the function of matrix vesicles in human skeletal tissues, which function as microcapsules embedded in ECM to create a compartmentalized environment for the nucleation and formation of bone minerals. 20 Fifth, microspheres made of cytocompatible polymers containing cell-adhesive peptide sequences can serve as a cell delivery vehicle by either cell encapsulation inside spheres or cell attachment at the exposed surface of the spheres.…”