Thermal study of simple amino-alcohol solutions

Abstract

Widely regarded as the most promising approach to long-term cryopreservation of organs for transplantation, vitrification is a process where liquid is transformed into a disordered solid state free from crystals, known as the amorphous state. The vitreous state is obtained by rapid cooling to cryogenic temperatures in the presence of antifreeze substances called cryoprotectants, such as polyalcohols, which are known to be very good vitrification agents. This work reports on the thermal properties of a new class of compounds, the amino-alcohols, studied for its similarity to the structure of the equivalent polyalcohols. We studied by differential scanning calorimetry the glass-forming tendency and stability of the amorphous state for de-ionized water solutions containing 2-amino-1-ethanol and 3-amino-1-propanol at the concentrations of 35%, 40%, 43%, and 45% (w/w). A comparison is made with previous results obtained by Mehl [Cryobiology 27 (1990) 687-688] on the same compounds under different experimental conditions. The results are also compared with those obtained by Boutron [Cryobiology 30 (1993) 87-97] for the corresponding dialcohols. A further comparison is made with a few results obtained for the 1-amino-2-propanol and the 2-amino-1-propanol tested under the same conditions. Ó 2002 Elsevier Science (USA). All rights reserved.

Effect of common cryoprotectants on critical warming rates and ice formation in aqueous solutions

“…As the warming rate q w increases, T m − T d decreases, and over the range of warming rates explored T m − T d varies roughly linearly with log(1/ q w ) [2,3,5,8–12,14–17,44,45,58]. Ice formation on warming at a given rate is reduced if the difference between T d and T m is small.…”

Technologies for Vitrification Based Cryopreservation

“…This is mainly because glass is a form of liquid with high viscosity. In a method reported in the literature, Boutron [46][47][48][49] and others [50][51][52][53] measure the glass transition temperature (T g ) based on a sudden change in heat capacity using a differential scanning calorimeter (DSC) [24].…”

Description of the hydrogen bond network in liquid 3-amino-1-propanol by graph theory and percolation methods

“…Aliphatic amino alcohols with a small bridge of СН 2 groups in the molecule can be used as inhibitors of crystallization of aqueous solutions; therefore, they are used in cryobiology as cryoprotectants for vitrification of biological materials [1]. They have a wide temperature range of the liquid phase and its strong hypothermia [2].…”