Thermoperiodic Control of Hypocotyl Elongation Depends on Auxin-Induced Ethylene Signaling That Controls Downstream <i>PHYTOCHROME INTERACTING FACTOR3</i> Activity

Bours, Ralph; Kohlen, Wouter; Bouwmeester, Harro J.; Krol, Alexander R. van der

doi:10.1104/pp.114.254425

Cited by 36 publications

(28 citation statements)

References 59 publications

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“…Indeed, we found that the expression of SAUR22 was higher at 25°C than at 18°C (Supplemental Fig. S13), which is consistent with previous reports indicating that SAUR19 subfamily members are induced by warm temperatures (Franklin et al, 2011;Delker et al, 2014;Johansson et al, 2014;Bours et al, 2015). This indicates that SAUR19 subfamily genes are regulated by temperature in our growth conditions.…”

Section: Sob3 Binds To the Promoters Of Genes Associated With Auxin Ssupporting

confidence: 81%

SUPPRESSOR OF PHYTOCHROME B4-#3 Represses Genes Associated with Auxin Signaling to Modulate Hypocotyl Growth

et al. 2016

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Developing seedlings are well equipped to alter their growth in response to external factors in order to maximize their chances of survival. SUPPRESSOR OF PHYTOCHROME B4-#3 (SOB3) and other members of the AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED (AHL) family of transcription factors modulate the development of Arabidopsis (Arabidopsis thaliana) by repressing hypocotyl elongation in young seedlings growing in light. However, the molecular mechanism behind how AHLs influence seedling development is largely unknown. We have identified genes associated with auxin-mediated hypocotyl elongation as downstream targets of SOB3. We found that YUCCA8 (YUC8) as well as members of the SMALL AUXIN UP-REGULATED RNA19 (SAUR19) subfamily were down-regulated in the short-hypocotyl, gain-of-function SOB3-D mutant and up-regulated in the dominant-negative, tall-hypocotyl sob3-6 mutant. SOB3-D and sob3-6 hypocotyls also exhibited altered sensitivity to the polar auxin transport inhibitor N-1-napthylphthalamic acid, suggesting a critical connection between auxin and the modulation of seedling elongation by SOB3. Finally, we found that overexpression of GREEN FLUORESCENT PROTEIN-SAUR19 in the SOB3-D line partially rescued defects in hypocotyl elongation, and SOB3 bound directly to the promoters of YUC8 and SAUR19 subfamily members. Taken together, these data indicate that SOB3 modulates hypocotyl elongation in young seedlings by directly repressing the transcription of genes associated with auxin signaling.

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Section: Sob3 Binds To the Promoters Of Genes Associated With Auxin Ssupporting

confidence: 81%

SUPPRESSOR OF PHYTOCHROME B4-#3 Represses Genes Associated with Auxin Signaling to Modulate Hypocotyl Growth

et al. 2016

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“…1A,B), suggesting that alteration of cortical microtubule orientation from transverse to longitudinal was significantly suppressed in ein2-5 cells in response to ACC. Multiple physiological processes are regulated by crosstalk between the responses to ethylene and auxin (Muday et al, 2012;Bours et al, 2015). We found that 1-naphthalene acetic acid (NAA) altered microtubule orientation in wild-type cells, although this effect was significantly suppressed in ein2-5 etiolated hypocotyl cells (Fig.…”

Section: Ethylene Signaling Participates In Regulation Of Cortical MImentioning

confidence: 94%

Microtubule bundling plays a role in ethylene-mediated cortical microtubule reorientation in etiolated Arabidopsis hypocotyls

Sun

Mao

2016

Journal of Cell Science

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The gaseous hormone ethylene is known to regulate plant growth under etiolated conditions (the 'triple response'). Although organization of cortical microtubules is essential for cell elongation, the underlying mechanisms that regulate microtubule organization by hormone signaling, including ethylene, are ambiguous. In the present study, we demonstrate that ethylene signaling participates in regulation of cortical microtubule reorientation. In particular, regulation of microtubule bundling is important for this process in etiolated hypocotyls. Time-lapse analysis indicated that selective stabilization of microtubule-bundling structures formed in various arrays is related to ethylene-mediated microtubule orientation. Bundling events and bundle growth lifetimes were significantly increased in oblique and longitudinal arrays, but decreased in transverse arrays in wild-type cells in response to ethylene. However, the effects of ethylene on microtubule bundling were partially suppressed in a microtubule-bundling protein WDL5 knockout mutant (wdl5-1). This study suggests that modulation of microtubule bundles that have formed in certain orientations plays a role in reorienting microtubule arrays in response to ethylenemediated etiolated hypocotyl cell elongation.

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“…Increasing evidence points to PIF3 playing a central role in promoting hypocotyl elongation under multiple abiotic and environmental conditions. For example, one study has shown thermoperiodic control of hypocotyl elongation through ethylene signaling-mediated regulation of PIF3 activity (Bours et al, 2015). Future studies are needed to investigate whether and how MDP60 functions in these processes.…”

Section: Light and Ethylene Coordinate Hypocotyl Cell Elongation Thromentioning

confidence: 99%

Coordinated Regulation of Hypocotyl Cell Elongation by Light and Ethylene through a Microtubule Destabilizing Protein

Wang

Sun

et al. 2017

Plant Physiol.

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Precise regulation of hypocotyl cell elongation is essential for plant growth and survival. Light suppresses hypocotyl elongation by degrading transcription factor phytochrome-interacting factor 3 (PIF3), whereas the phytohormone ethylene promotes hypocotyl elongation by activating PIF3. However, the underlying mechanisms regarding how these two pathways coordinate downstream effectors to mediate hypocotyl elongation are largely unclear. In this study, we identified the novel MicrotubuleDestabilizing Protein 60 (MDP60), which plays a positive role in hypocotyl cell elongation in Arabidopsis (Arabidopsis thaliana); this effect is mediated through PIF3. Ethylene signaling up-regulates MDP60 expression via PIF3 binding to the MDP60 promoter. MDP60 loss-of-function mutants exhibit much shorter hypocotyls, whereas MDP60 overexpression significantly promotes hypocotyl cell elongation when grown in light compared to the control. MDP60 protein binds to microtubules in vitro and in vivo. The organization of cortical microtubules was significantly disrupted in mdp60 mutant cells and MDP60-overexpressing seedlings. These findings indicate that MDP60 is an important mediator of hypocotyl cell elongation. This study reveals a mechanism in which light and ethylene signaling coordinate MDP60 expression to modulate hypocotyl cell elongation by altering cortical microtubules in Arabidopsis.

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Thermoperiodic Control of Hypocotyl Elongation Depends on Auxin-Induced Ethylene Signaling That Controls Downstream PHYTOCHROME INTERACTING FACTOR3 Activity

Cited by 36 publications

References 59 publications

SUPPRESSOR OF PHYTOCHROME B4-#3 Represses Genes Associated with Auxin Signaling to Modulate Hypocotyl Growth

SUPPRESSOR OF PHYTOCHROME B4-#3 Represses Genes Associated with Auxin Signaling to Modulate Hypocotyl Growth

Microtubule bundling plays a role in ethylene-mediated cortical microtubule reorientation in etiolated Arabidopsis hypocotyls

Coordinated Regulation of Hypocotyl Cell Elongation by Light and Ethylene through a Microtubule Destabilizing Protein

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