Thermophilic protease production by Streptomyces sp. 594 in submerged and solid-state fermentations using feather meal

Abstract

Aims: Protease production by Streptomyces sp. 594 in submerged (SF) and solid‐state fermentation (SSF) using feather meal, an industrial poultry residue, and partial characterization of the crude enzyme. Methods and Results: Streptomyces sp. 594 produced proteases in SF (7·2 ± 0·2 U ml−1) and SSF (15·5 ± 0·41 U g−1), with pH increase in both media. Considering protease activity, values obtained in the liquid extract after SSF (6·3 ± 0·17 U ml−1) were lower than those from SF. The proteases, which belong to serine and metalloproteinase classes, were active over a wide range of pH (5·0–10·0) and high temperatures (55–80°C). Strain 594 was also able to degrade feather in agar and liquid media. Keratinase activity (80 U l−1) also confirmed the keratin degrading capacity of this streptomycete. Conclusions: Proteases produced using residues from poultry industry have shown interesting properties for industrial purposes. Significance and Impact of the Study: As far as we are concerned, this is the first contribution towards the production of thermophilic protease by a streptomycete in SSF using a keratinous waste.

Production and optimization of thermophilic alkaline protease in solid-state fermentation by Streptomyces sp. CN902

“…Various substrates for optimal enzyme production have been tested including feather meal for thermophilic protease production by Streptomyces sp. 592 [22], wheat bran for -amylase by Penicillium chrysogenum [21], red gram plant waste for -galactosidase by Aspergillus oryrae [23]. In the present work, Streptomyces sp.…”

Medium optimization for keratinase production by a local Streptomyces sp. NRC 13S under solid state fermentation

“…Previous studies dealing with the effect of initial pH on protease production by other Streptomyces such as Streptomyces rimosus and Streptomyces sp. 594 in SSF conditions indicated lower pH values, i.e., between 6 and 7 for optimal protease activity (De Azeredo et al, 2005). Moreover, pH value of 9 and 10 were found to be suitable for optimal alkaline protease by Streptomyces sp.…”

“…Other reports indicated the requirements of 60% (w/w) initial moisture content for maximum protease production by Streptomyces sp. 594 (De Azeredo et al, 2005) and Streptomyces sp. CN902 (Lazim et al, 2009).…”

Optimization of Extraction Parameters for Keratinase Recovery from Fermented Feather under Solid State Fermentation by Streptomyces sp. NRC 13S

“…Different solvents were tested for extraction of keratinase: distilled water, tap water, glycerol (1% v/v), 0.1 M glycine buffer (pH 8, 9, 10, 11), 0.1 M sodium phosphate buffer (pH 6.0, 7.0, 8.0), NaCl solution (10% w/v), and non-ionic detergents such as Tween 20, Tween 40, Tween 60, Tween 80 and Triton X-100. The kinds of solvents, the range of pH and concentration were chosen based on some previous studies of extraction of proteases (Tunga et al, 1999;Aikat and Bhattacharyya, 2000;De Azeredo et al, 2006;Sumantha et al, 2006). Keratinase assay.…”