Thermostable alkaline protease from newly isolated Vibrio sp.: extraction, purification and characterisation

Subhashini, Ponnambalam; Annamalai, Neelamegam; Saravanakumar, A.; Balasubramanian, T.

doi:10.2478/s11756-012-0067-0

Cited by 8 publications

(1 citation statement)

References 37 publications

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“…Usually, the optimum catalysis pH for alkaline proteases (APases) ranges from 8.0 to 10.0. [34][35][36] The protease showed activity over a wide range of pH values for casein substrate hydrolysis and exhibited maximum activity at pH 10.0 (Fig. 4(A)).…”

Section: Effect Of Ph Temperature and Salinity On Protease Activitymentioning

confidence: 99%

Genome sequencing of cold‐adapted Planococcus bacterium isolated from traditional shrimp paste and protease identification

Leng

Gao

et al. 2020

J Sci Food Agric

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BACKGROUND: Psychrophiles have evolved to adapt to freezing environments, and cold-adapted enzymes from these organisms can maintain high catalytic activity at low temperature. The use of cold-adapted enzymes has great potential for the revolution of food and molecular biology industries.RESULTS: In this study, four different strains producing protease were isolated from traditional fermented shrimp paste, one of which, named Planococcus maritimus XJ11 by 16S rRNA nucleotide sequence analysis, exhibited the largest protein hydrolysis clear zone surrounding the colonies. Meanwhile, the strain P. maritimus XJ11 was selected for further investigation because of its great adaptation to low temperature, low salinity and alkaline environment. The enzyme activity assay of P. maritimus XJ11 indicated that the optimum conditions for catalytic activity were pH 10.0 and 40°C. Moreover, the enzyme also showed an increasing activity with temperatures from 10 to 40°C and retained more than 67% activity of the maximum over a broad range of salinity (50-150 g L −1 ). Genome sequencing analysis revealed that strain XJ11 possessed one circular chromosome of 3 282 604 bp and one circular plasmid of 67 339 bp, with a total number of 3293 open reading frames (ORFs). Besides, 21 genes encoding protease, including three serine proteases, were identified through the NR database.CONCLUSION: Cold-adapted bacterium P. maritimus XJ11 was capable of producing alkaline proteases with high catalytic efficiency at low or moderate temperatures. Furthermore, the favorable psychrophilic and enzymatic characters of strain P. maritimus XJ11 seem to have a promising potential for industrial application.

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Section: Effect Of Ph Temperature and Salinity On Protease Activitymentioning

confidence: 99%

Genome sequencing of cold‐adapted Planococcus bacterium isolated from traditional shrimp paste and protease identification

Leng

Gao

et al. 2020

J Sci Food Agric

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Optimization of Protease Enzyme Production by the Halo-Tolerant Vibrio alginolyticus Isolated from Marine Sources

Malathi

Priya

Palani

2014

Microbial Diversity and Biotechnology in Food Security

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A renewed interest in the study of proteolytic enzymes is chiefly attributable to its surging industrial applications apart from its pivotal role in the cellular metabolic process. We report the screening, isolation and partial purification of a low-molecular-weight halo-tolerant alkaline protease from a bacterial isolate of marine environment. Twenty four bacterial isolates were collected from marine environment, of which Vibrio alginolyticus showed relatively higher proteolytic activity on agar plate and was subjected to further characterization. Enzyme production kinetics under submerged fermentation was optimized, that included incubation time, pH and heavy metal tolerance. The bacterium produced the highest protease activity (4650 U/mL) when incubated for 48 h at pH 8.0. The organism was found to be more tolerant to iron than other metals like Cu 2+ , Mn2 + , Zn 2+ , Co 2+ and Hg 2+ . Natural substrates such as rice and wheat brans were evaluated for protease enzyme production under solid state fermentation. A mixture of rice and wheat bran at a ratio of 1:1 supported the higher enzyme production. Effect of NaCl, heavy metals and EDTA on the catalytic activity of the crude enzyme was assayed. The total activity of the crude enzyme was unaffected at elevated concentrations of NaCl and retained 40 % of its activity even at 4 M concentration which indicated the halo-tolerant ability of the bacterium. An increase in the catalytic activity (about 30 % or 1.2 fold) was recorded when incubated with 1 mM of Co 2+ while considerable loss in function was recorded with other heavy metals. Similar loss of function has been recorded with EDTA indicating that it is a metal ion-dependent enzyme. The partially purified enzyme was able to digest the meat and showed high capability of removing blood stains from fabric. KeywordsVibrio alginolyticus · Protease · Halo-tolerance · Marine bacterium

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Purification and Characterization of a Cold Alkaline Protease from a Psychrophilic Pseudomonas aeruginosa HY1215

Hao

Sun

2014

Appl Biochem Biotechnol

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A novel alkaline protease was purified from Pseudomonas aeruginosa HY1215 using ammonium sulfate, DEAE-Sepharose and Sephacryl S-200 gel filtration chromatographic techniques. The protease had a relative molecular weight of 32.8 KDa by SDS-PAGE, and the optimal temperature and pH for excellent stability and activity were determined as 25 °C and 10.0, respectively. Within the pH range of 7.0-11.0, the protease had a good stability, which could retain more than 80 % of its original activity; in the temperature range of 15-35 °C, the protease had a higher activity, and its activity at 20 °C could amount to 85 % of the maximum activity at 25 °C. Besides, the enzyme activity showed a valuable stability towards several commercially available surfactants (Tween-80, Tween-40, and Triton X-100) and bleaches (H2O2) even when their concentrations ranged up to 2.0 and 1.6 %.

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Thermostable alkaline protease from newly isolated Vibrio sp.: extraction, purification and characterisation

Cited by 8 publications

References 37 publications

Genome sequencing of cold‐adapted Planococcus bacterium isolated from traditional shrimp paste and protease identification

Genome sequencing of cold‐adapted Planococcus bacterium isolated from traditional shrimp paste and protease identification

Optimization of Protease Enzyme Production by the Halo-Tolerant Vibrio alginolyticus Isolated from Marine Sources

Purification and Characterization of a Cold Alkaline Protease from a Psychrophilic Pseudomonas aeruginosa HY1215

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