Thidiazuron-induced hypertrophic growth from foliar disks of Kalanchoe pinnata leads to visualization of a bioactive auxin gradient across the leaf plane

Jaiswal, Sarita; Sawhney, S.

doi:10.1007/s11627-007-9084-z

Cited by 3 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More and more researches indicated that the action mechanism of TDZ can be closely linked to the biosynthesis and transportation of IAA. Jaiswal and Sawhney (2008) observed an organized hypertrophic growth at the vein ending on the proximal side of K. pinnata leaf disks induced by TDZ, which is considered an exclusive auxin-mediated response. Further experiment proved that the effect is completely reversed by auxin efflux inhibitor, 2,3,5-triiodobenzoic acid (TIBA).…”

Section: Involvement Of Phytohormonesmentioning

confidence: 89%

Thidiazuron: A multi-dimensional plant growth regulator

Guo¹,

Abbasi²,

Zeb³

et al. 2011

Afr. J. Biotechnol.

200

View full text Add to dashboard Cite

Thidiazuron (TDZ) has gained a considerable attention during past decades due to its efficient role in plant cell and tissue culture. Wide array of physiological responses were observed in response to TDZapplication in different plant species. TDZ has shown both auxin and cytokinin like effects, although, chemically, it is totally different from commonly used auxins and cytokinins. A number of biological (physiological and biochemical) events in cells are induced or enhanced by TDZ, but the mode of action of TDZ is yet unknown. However, varieties of underlying mechanisms were revealed by reports showing how morphogenic events were induced by application of TDZ. Other reports showed that TDZ may modify endogenous plant growth regulators, either directly or indirectly and produce reactions in cell/tissue, necessary for its division/regeneration. Other possibilities include modification in cell membrane, energy levels, nutrient absorption, transport and assimilation, etc. In this review, recent advancements in TDZ application in plant sciences are discussed.

show abstract

Section: Involvement Of Phytohormonesmentioning

confidence: 89%

Thidiazuron: A multi-dimensional plant growth regulator

Guo¹,

Abbasi²,

Zeb³

et al. 2011

Afr. J. Biotechnol.

200

View full text Add to dashboard Cite

show abstract

“…In addition, it exhibits clonal regeneration from roots and, more importantly, from detached leaves. New clonal plantlets are potentially formed from all undifferentiated meristem in the marginal notches of the crenated leaves (Jaiswal and Sawhney ).…”

Section: Methodsmentioning

confidence: 99%

Mating system, population growth, and management scenario for Kalanchoe pinnata in an invaded seasonally dry tropical forest

León

Herrera

Guevara

2016

Ecology and Evolution

View full text Add to dashboard Cite

Ecological invasions are a major issue worldwide, where successful invasion depends on traits that facilitate dispersion, establishment, and population growth. The nonnative succulent plant Kalanchoe pinnata, reported as invasive in some countries, is widespread in remnants of seasonally dry tropical forest on a volcanic outcrop with high conservation value in east‐central Mexico where we assessed its mating system and demographic growth and identified management strategies. To understand its local mating system, we conducted hand‐pollination treatments, germination, and survival experiments. Based on the experimental data, we constructed a life‐stage population matrix, identified the key traits for population growth, weighted the contributions of vegetative and sexual reproduction, and evaluated management scenarios. Hand‐pollination treatments had slight effects on fruit and seed setting, as well as on germination. With natural pollination treatment, the successful germination of seeds from only 2/39 fruit suggests occasional effective natural cross‐pollination. The ratios of the metrics for self‐ and cross‐pollinated flowers suggest that K. pinnata is partially self‐compatible. Most of the pollinated flowers developed into fruit, but the seed germination and seedling survival rates were low. Thus, vegetative propagation and juvenile survival are the main drivers of population growth. Simulations of a virtual K. pinnata population suggest that an intense and sustained weeding campaign will reduce the population within at least 10 years. Synthesis and applications. The study population is partially self‐compatible, but sexual reproduction by K. pinnata is limited at the study site, and population growth is supported by vegetative propagation and juvenile survival. Demographic modeling provides key insights and realistic forecasts on invasion process and therefore is useful to design management strategies.

show abstract

“…Secondly, although kinetin is an adenine-type, synthetic, more stable cytokinin that persists in plants, it is also regulated by cytokinin oxidase (Nogue et al, 2003) and this could be a probable reason why media containing kinetin or ZR resulted in comparable numbers of cultivars with regeneration capacity (Yamada et al, 2001;Osugi and Sakakibara, 2015;Bhatla and Lal, 2018). Thirdly, TDZ is a synthetic phenyl-urea that inhibits the activity of cytokinin oxidase, probably due to its auxin-like properties and persistence in plants (Bilyeu et al, 2001;Nogue et al, 2003;Jaiswal and Sawhney, 2008). However, some cultivars produced RE > 10% on media containing TDZ, indicative of type on RE was evident, our data clearly demonstrated that genotype plays a more important role in RE than cytokinin type.…”

Section: Genotype and Cytokinin Dependentmentioning

confidence: 99%