Thin‐layer chromatographic determination of antioxidants by the stable free radical ॅ, ॅ′‐diphenyl‐ॆ‐picrylhydrazyl

Glavind, J.; Hølmer, Gunhild

doi:10.1007/bf02679243

Cited by 43 publications

(17 citation statements)

References 9 publications

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“…Thus it is no longer necessary to purify every single constituent for off-line assays, leading to very significant reductions of cost and faster results. f. Thin Layer Chromatography (TLC)-DPPH methods TLC combined with DPPH radical detection (Glavind and Holmer 1967) involved TLC separation of the analyte, followed by detection of DPPH radical scavengers by spraying the TLC plates with DPPH solution. The plates were evaluated visually and a quantitative determination could be achieved by eluting the spots with DPPH reagent (Takao et al 1994).…”

Section: Developments In Dpph Methodsmentioning

confidence: 99%

Genesis and development of DPPH method of antioxidant assay

2011

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α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method offers the first approach for evaluating the antioxidant potential of a compound, an extract or other biological sources. This is the simplest method, wherein the prospective compound or extract is mixed with DPPH solution and absorbance is recorded after a defined period. However, with the advancement and sophistication in instrumental techniques, the method has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them. This article presents a critical review on various developments to the DPPH method.

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Section: Developments In Dpph Methodsmentioning

confidence: 99%

Genesis and development of DPPH method of antioxidant assay

2011

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“…The n-hexane/EtOAc (1:1) eluate fraction showed DPPH radical-scavenging activity in the TLC assay. 8 This fraction was further applied to a silica gel column and eluted with n-hexane/EtOAc (2:1). The active fraction was purified by repeated Sephadex LH-20 column chromatography (MeOH), and the fraction containing DPPH radical-scavenging compounds was purified using preparative ODS HPLC (CH 3 CN-H 2 O-TFA).…”

Section: Isolation Of Compoundmentioning

confidence: 99%

Surugapyrone A from Streptomyces coelicoflavus strain USF-6280 as a new DPPH radical-scavenger

et al. 2010

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Surugapyrone A, a novel 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging 2-pyrone, was isolated from the culture filtrate of an actinomycete strain USF-6280. This strain was assigned to the genus Streptomyces owing to its morphological and chemotaxonomic characteristics. Phylogenetic analysis based on 16S ribosomal RNA gene sequences supported the classification of the producing strain as a member of the genus Streptomyces and indicated that it was closely related to Streptomyces coelicoflavus. As the morphological, physiological and biochemical characteristics of the producing strain were in agreement with those of the type strain of S. coelicoflavus, we concluded that strain USF-6280 should be identified as a member of S. coelicoflavus. The structure of surugapyrone A was determined to be 4-hydroxy-6-isopropyl-3-methyl-2-pyrone on the basis of the spectroscopic data. The results of feeding experiments with 13 C-labeled compounds indicated that surugapyrone A was biosynthesized through a polyketide pathway involving isobutyrate, acetate and propionate.

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“…TLC combined with DPPH9 radical detection of antioxidants in situ was first introduced in 1967 by Glavind and Holmer [11]. A similar method both on normal phase [12] and on RP [13] TLC was used for screening antioxidants present in plant extracts.…”

Section: Introductionmentioning

confidence: 99%

Separation and evaluation of free radical‐scavenging activity of phenol components of Emblica officinalis extract by using an HPTLC–DPPH^• method

Pozharitskaya¹,

Иванова²,

Shikov³

et al. 2007

J of Separation Science

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A new procedure has been developed to separate and quantify the free radical-scavenging activity of individual compounds from an Emblica officinalis extract based on the combination of HPTLC with a diode array detector (DAD) and postchromatographic DPPH* radical derivatization. Free gallic and ellagic acids and emblicanins A and B in the E. officinalis extract were separated by TLC and identified. All the compounds of the extract were capable of scavenging of DPPH* radicals. It was established that the DPPH* scavenging activity of emblicanins A and B was 7.86 and 11.20 times more than that of ascorbic acid and 1.25 and 1.78 times more than gallic acid, respectively. From the estimated ID50 values, it can be seen that the increasing order of activity was emblicanin B > emblicanin A > gallic acid > ellagic acid > ascorbic acid. Probably, the antioxidant activity of E. officinalis extract is associated with the presence of hydrolyzable tannins having ascorbic acid-like action.

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Thin‐layer chromatographic determination of antioxidants by the stable free radical ॅ, ॅ′‐diphenyl‐ॆ‐picrylhydrazyl

Cited by 43 publications

References 9 publications

Genesis and development of DPPH method of antioxidant assay

Genesis and development of DPPH method of antioxidant assay

Surugapyrone A from Streptomyces coelicoflavus strain USF-6280 as a new DPPH radical-scavenger

Separation and evaluation of free radical‐scavenging activity of phenol components of Emblica officinalis extract by using an HPTLC–DPPH^• method

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Thin‐layer chromatographic determination of antioxidants by the stable free radical ॅ, ॅ′‐diphenyl‐ॆ‐picrylhydrazyl

Cited by 43 publications

References 9 publications

Genesis and development of DPPH method of antioxidant assay

Genesis and development of DPPH method of antioxidant assay

Surugapyrone A from Streptomyces coelicoflavus strain USF-6280 as a new DPPH radical-scavenger

Separation and evaluation of free radical‐scavenging activity of phenol components of Emblica officinalis extract by using an HPTLC–DPPH• method

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Separation and evaluation of free radical‐scavenging activity of phenol components of Emblica officinalis extract by using an HPTLC–DPPH^• method