Thin-Layer Gel Chromatography

Abstract

225 INTRODUCTION 226 PECULIARITY OF THIN-LAYER GEL CHROMATOGRAPHY 226 EXPERIMENTAL TECHNIQUES 228 Development apparatus 228 Preparation of gel plates 231 Sample application 232 Development 233 Detection 235 INTERPRETATION OF CHROMATOGRAMS 237 APPLICATIONS 239 Molecular weight and size estimations 239 Biological macro molecules 242 Lypophilic substances 245 Inorganic substances 246 REFERENCES 252 ABSTRACTThin-layer gel chromatography (TLGC), in which the separation is achieved on the basis of molecular size, has all the advantages offered by the thin-layer technique, i.e., speed, sensitivity and simplicity. TLGC can be carried out only when the pores of the gel materials are filled with the eluent solvent, so that this method requires a number of special experimental techniques different from the usual thin-layer chromatography. This review describes an essential peculiarity of TLGC and the experimental techniques involved, and emphasizes recent applications of TLGC to lypophilic substances and inorganic substances, although most of the published applications are concerned with biological materials. 225Brought to you by | Purdue University Librarie Authenticated Download Date | 7/2/15 10:16 AM