Thiosemicarbazide Used After Periodic Acid Makes Methenamine Silver Staining of Renal Glomerular Basement Membranes Faster and Cleaner

Abstract

The periodic acid-methenamine silver staining technique, which is frequently used for demonstrating the renal glomerular basement membrane, require a high degree of skill, and in some cases it may be difficult t o obtain a p o d result To overcome such difficulty and inconsistency, we have improved the method by performing methenamine silver staining after oxidation with periodic acid and subsequent application of thi-aubazide.In this procedure, this semicarbazide enhanced the reaction of methenamine silver with the glomcrular basement membrane and the reaction was completed within a shorter time in comparison with the conventional method. This modification also eliminated any nonspecific W o n with the surface of the glass slide and the solution container and yielded excellent and reproducible results irrespective of the fixation method and material employed. It wpll also. found to stain the renal glomerular basement membrane of rabbits, which is demonstrable only with difficulty by the conventional method. ABSTRACT.Biotech Histochem Downloaded from informahealthcare.com by Nyu Medical Center on 11/03/14For personal use only.

Compound effects of aging and experimental FSGS on glomerular epithelial cells

“…Because of the different responses in both types of glomerular epithelial cells between young and aged mice in the same model of FSGS, we next examined extracellular matrix accumulation, by staining for collagen IV (Col IV) (Figure 7A-L), and Jones’ basement membrane stain (methenamine silver-Periodic acid Schiff stain, Silver Stain) [37, 38] (Figure 7M-X). Table 2 shows semi-quantitation for Col IV staining performed with two specific questions in mind: are there differences between OC, JM and total glomeruli that stain for Col IV, and within OC and JM glomeruli, are there differences between intra-glomerular staining patterns?…”

Effect of ADAM28 on Carcinoma Cell Metastasis by Cleavage of von Willebrand Factor

“…The formalin-fi xed lung tissues were embedded in paraffin , and paraffin sections were subjected to HE staining and immunostaining for Venus as described above. To examine the apoptosis of PC-9 ffLuc-cp156 cells within the lung tissues obtained from mice killed at 1 and 3 days after intravenous injection, serial sections (4 μ m thick) were made and stained with HE and thiosemicarbazide-periodic acid methenamine silver-hematoxylin (TSC-PAM) ( 23 ). They were also subjected to immunohistochemistry for cleaved CASP3 protein using rabbit anti-human cleaved CASP3 polyclonal antibody (20 μ g/mL) (Millipore), followed by reactions with EnVision system HRP-labeled polymer anti-rabbit IgG (1:200 dilution) (Dako).…”

New Grocott Stain without Using Chromic Acid

“…We also referred to the report by Hayashi et al [ 8 ], who improved PAM stain by enhancing the silver reaction through the pretreatment with thiosemicarbazide after periodic acid oxidization. However, thiosemicarbazide belongs to the hazardous chemical category and so we abandoned using it.…”