Thirty-three microsatellite loci for noninvasive genetic studies of the giant panda (Ailuropoda melanoleuca)

Wu, Hua; Zhan, Xiangjiang; Zhang, Zejun; Zhu, Lifeng; Li, Yan; Li, Ming; Wei, Fuwen

doi:10.1007/s10592-008-9599-9

Cited by 54 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, microsatellite makers are widely utilized in genetic variation studies for endangered species due to its high levels of variations, noninvasive sampling technique, and small amount of template DNA required (Jarne & Lagoda 1996;Beaumont & Bruford 1999). In previous studies, 79 microsatellite loci have been developed for giant panda (Zhang, Wang & Su, 1995;Lu, Johnson & Menotti-Raymond, 2001;Shen et al, 2005Shen et al, , 2007Zhang et al, 2009;Wu et al, 2009). However, most of the previously published microsatellite markers are dinucleotide repeats.…”

Section: Discussionmentioning

confidence: 99%

Development of new tetranucleotide microsatellite loci and assessment of genetic variation of giant panda in two largest giant panda captive breeding populations

Shen

et al. 2010

Journal of Zoology

View full text Add to dashboard Cite

The giant panda Ailuropoda melanoleuca is a critically endangered species endemic to China. In order to carry out effective genetic management for the giant panda population, sufficient and reliable polymorphic genetic markers are required to provide essential information on the genetic diversity survey of this species. Seven new tetranucleotide microsatellite loci were isolated and characterized in this study and presented here as a tool for evaluating the genetic variation of giant pandas in the world's two largest captive populations (Chengdu Research Base of Giant Panda Breeding, Sichuan Province and the China Research and Conservation Center for the Giant Panda in Wolong, Sichuan Province). A total of 45 alleles were identified from these seven new microsatellite loci on the basis of 48 giant panda individuals, including 19 private alleles (six from the Chengdu population, 13 from the Wolong population) and 26 shared alleles. The average number of alleles, the average allelic richness the and mean observed heterozygosity were 4.6, 4.367 and 0.649, respectively in the Chengdu population and 5.6, 5.697 and 0.675 in the Wolong population, suggesting that the Chengdu population has a much lower allelic diversity than the Wolong population. Thus, we proposed a better strategy for the captive breeding of giant pandas.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of new tetranucleotide microsatellite loci and assessment of genetic variation of giant panda in two largest giant panda captive breeding populations

Shen

et al. 2010

Journal of Zoology

View full text Add to dashboard Cite

show abstract

“…; Wu et al . ) and the trend towards combining multiple molecular marker types, the picture of panda genetic diversity started to change during the 2000s. Lü et al .…”

Section: Genetic Diversitymentioning

confidence: 99%

Black and white and read all over: the past, present and future of giant panda genetics

et al. 2012

View full text Add to dashboard Cite

Few species attract much more attention from the public and scientists than the giant panda (Ailuropoda melanoleuca), a popular, enigmatic but highly endangered species. The application of molecular genetics to its biology and conservation has facilitated surprising insights into the biology of giant pandas as well as the effectiveness of conservation efforts during the past decades. Here, we review the history of genetic advances in this species, from phylogeny, demographical history, genetic variation, population structure, noninvasive population census and adaptive evolution to reveal to what extent the current status of the giant panda is a reflection of its evolutionary legacy, as opposed to the influence of anthropogenic factors that have negatively impacted this species. In addition, we summarize the conservation implications of these genetic findings applied for the management of this high-profile species. Finally, on the basis of these advances and predictable future changes in genetic technology, we discuss future research directions that seem promising for giant panda biology and conservation.

show abstract

“…Twelve microsatellite loci: Ame-l5, l10, l13, l15, l22, l26 , AY116213 (Shen et al 2005), Amel11, l24, AY79, AY95, AY217 (redesigned loci; Wu et al 2009) were used to amplify DNA extracts from fecal and hair samples. To obtain reliable genotypes, a stringent multi-tube approach was used (Taberlet et al 1996).…”

Section: Molecular Analysismentioning

confidence: 99%

Spatial genetic structure and dispersal of giant pandas on a mountain-range scale

Zhan

et al. 2010

Conserv Genet

View full text Add to dashboard Cite

Understanding the spatial genetic structure of populations can provide insight into the ecological or evolutionary processes of the species, and enable wise conservation decisions. We examined the spatial genetic structure of a giant panda (Ailuropoda melanoleuca) population in a heterogeneous mountainous landscape using noninvasive genetic sampling and 12 microsatellite loci. Nonrandom genetic structure was detected through spatial autocorrelation analysis, demonstrating a significantly positive autocorrelation over closer distances. Additional spatial analyses showed significantly positive genetic correlation among spatially-proximate males, and no correlation among females and among male-female pairs. These findings suggest a female-biased dispersal pattern and cryptic family grouping among giant pandas on a large mountain-range scale. The spatial extent of genetic structure occurred within 12.5 km, measured by a least-cost path distance model integrating information of habitat quality and habitat preferences of this species. Using the bearing analysis of PASSAGE, we found that directional genetic autocorrelations were in agreement with habitat structure, and habitat heterogeneity may affect the direction of giant panda dispersal. The characterization of spatial genetic structure can provide potentially valuable information for the conservation and management of giant pandas and their habitat.

show abstract

Thirty-three microsatellite loci for noninvasive genetic studies of the giant panda (Ailuropoda melanoleuca)

Cited by 54 publications

References 21 publications

Development of new tetranucleotide microsatellite loci and assessment of genetic variation of giant panda in two largest giant panda captive breeding populations

Development of new tetranucleotide microsatellite loci and assessment of genetic variation of giant panda in two largest giant panda captive breeding populations

Black and white and read all over: the past, present and future of giant panda genetics

Spatial genetic structure and dispersal of giant pandas on a mountain-range scale

Contact Info

Product

Resources

About