Three-dimensional scaffolds for bioengineering of cartilage tissue

Wasyłeczko, Monika; Krysiak, Zuzanna J.; Łukowska, Ewa; Gruba, Marcin; Sikorska, Wioleta; Kruk, Aleksandra; Dulnik, Judyta; Czubak, Jarosław; Chwojnowski, Andrzej

doi:10.1016/j.bbe.2022.03.004

Cited by 7 publications

(17 citation statements)

References 127 publications

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“…The top layer was perforated, allowing cells to enter the membrane. Their cross-section was characterized by a unique spatial structure formed by an interconnected network of pores [ 7 , 36 , 62 ]. Their main task, in addition to keeping cells in space, is to provide an environment as similar as possible to that naturally occurring in the human body.…”

Section: Resultsmentioning

confidence: 99%

“…A development of new membranes permits the replacement of transplants or provides new alternative methods allowing the treatment of damaged tissues or even diseases such as diabetes [ 1 , 2 , 3 ]. For example, flat membranes can be used for skin regeneration [ 4 , 5 ], the spatial form of membranes (scaffolds) are used for cell support during cultivation [ 6 , 7 , 8 , 9 ], and hollow fiber membranes (HFMs) are used in dialysis [ 10 ]. Membranes are also used to encapsulate active ingredients/cells in a drug delivery system (DDS) [ 1 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Synthetic polymers, compared to natural polymers, have better mechanical resistance and durability under various conditions. The most commonly used synthetic polymers in scaffold development are polylactic acid (PLA) [ 51 , 52 ], polycaprolactone (PCL) [ 53 , 54 ], polyurethane (PUR) [ 55 , 56 ], and polyethersulfone (PES) [ 7 , 57 , 58 , 59 ]. Due to their good mechanical, physical, and chemical properties, they can be used to produce various scaffold shapes using different techniques.…”

Section: Introductionmentioning

confidence: 99%

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Scaffolds for Cartilage Tissue Engineering from a Blend of Polyethersulfone and Polyurethane Polymers

et al. 2023

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In recent years, one of the main goals of cartilage tissue engineering has been to find appropriate scaffolds for hyaline cartilage regeneration, which could serve as a matrix for chondrocytes or stem cell cultures. The study presents three types of scaffolds obtained from a blend of polyethersulfone (PES) and polyurethane (PUR) by a combination of wet-phase inversion and salt-leaching methods. The nonwovens made of gelatin and sodium chloride (NaCl) were used as precursors of macropores. Thus, obtained membranes were characterized by a suitable structure. The top layers were perforated, with pores over 20 µm, which allows cells to enter the membrane. The use of a nonwoven made it possible to develop a three-dimensional network of interconnected macropores that is required for cell activity and mobility. Examination of wettability (contact angle, swelling ratio) showed a hydrophilic nature of scaffolds. The mechanical test showed that the scaffolds were suitable for knee joint applications (stress above 10 MPa). Next, the scaffolds underwent a degradation study in simulated body fluid (SBF). Weight loss after four weeks and changes in structure were assessed using scanning electron microscopy (SEM) and MeMoExplorer Software, a program that estimates the size of pores. The porosity measurements after degradation confirmed an increase in pore size, as expected. Hydrolysis was confirmed by Fourier-transform infrared spectroscopy (FT-IR) analysis, where the disappearance of ester bonds at about 1730 cm −1 wavelength is noticeable after degradation. The obtained results showed that the scaffolds meet the requirements for cartilage tissue engineering membranes and should undergo further testing on an animal model.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Scaffolds for Cartilage Tissue Engineering from a Blend of Polyethersulfone and Polyurethane Polymers

et al. 2023

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“…Some studies also illustrate the utilization of this novel technology for producing exoskeletons, ears, stem cells, bones and microvascular networks [15] , [16] , [17] . The technology utilizes different biomaterials including metals, powders, liquids, ceramics, polymers and living cells to develop intricate structures with excellent mechanical characteristics, which cannot be attained through conventional manufacturing techniques [18] , [19] , [20] . Biomaterials used for the development of such implants and human organs can be classified into three types of materials, i.e., metals, polymers and ceramics [21] .…”

Section: Introductionmentioning

confidence: 99%

Additive manufacturing of sustainable biomaterials for biomedical applications

Arif

Khalid

Noroozi

et al. 2023

Asian Journal of Pharmaceutical Sciences

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“…In addition, methods for obtaining scaffolds from these materials are limited due to their lack of resistance to process parameters, like high temperature or pressure [ 9 , 16 , 24 , 25 , 26 , 27 , 28 , 33 ]. Synthetic polymers: polycaprolactone (PCL), polyurethane, polylactic acid (PLA), and polyethersulfone (PES) [ 21 , 31 , 38 , 39 , 40 , 41 , 42 , 43 ] are more diverse and promising. Compared to natural materials, they can be used to produce a variety of membrane structures using different methods.…”

Section: Introductionmentioning

confidence: 99%

Intraarticular Implantation of Autologous Chondrocytes Placed on Collagen or Polyethersulfone Scaffolds: An Experimental Study in Rabbits

et al. 2023

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Hyaline cartilage has very limited repair capability and cannot be rebuilt predictably using conventional treatments. This study presents Autologous Chondrocyte Implantation (ACI) on two different scaffolds for the treatment of lesions in hyaline cartilage in rabbits. The first one is a commercially available scaffold (Chondro–Gide) made of collagen type I/III and the second one is a polyethersulfone (PES) synthetic membrane, manufactured by phase inversion. The revolutionary idea in the present study is the fact that we used PES membranes, which have unique features and benefits that are desirable for the 3D cultivation of chondrocytes. Sixty-four White New Zealand rabbits were used in this research. Defects penetrating into the subchondral bone were filled with or without the placement of chondrocytes on collagen or PES membranes after two weeks of culture. The expression of the gene encoding type II procollagen, a molecular marker of chondrocytes, was evaluated. Elemental analysis was performed to estimate the weight of tissue grown on the PES membrane. The reparative tissue was analyzed macroscopically and histologically after surgery at 12, 25, and 52 weeks. RT-PCR analysis of the mRNA isolated from cells detached from the polysulphonic membrane revealed the expression of type II procollagen. The elementary analysis of polysulphonic membrane slices after 2 weeks of culture with chondrocytes revealed a concentration of 0.23 mg of tissue on one part of the membrane. Macroscopic and microscopic evaluation indicated that the quality of regenerated tissue was similar after the transplantation of cells placed on polysulphonic or collagen membranes. The established method for the culture and transplantation of chondrocytes placed on polysulphonic membranes resulted in the growth of the regenerated tissue, revealing the morphology of hyaline-like cartilage to be of similar quality to collagen membranes.

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Three-dimensional scaffolds for bioengineering of cartilage tissue

Cited by 7 publications

References 127 publications

Scaffolds for Cartilage Tissue Engineering from a Blend of Polyethersulfone and Polyurethane Polymers

Scaffolds for Cartilage Tissue Engineering from a Blend of Polyethersulfone and Polyurethane Polymers

Additive manufacturing of sustainable biomaterials for biomedical applications

Intraarticular Implantation of Autologous Chondrocytes Placed on Collagen or Polyethersulfone Scaffolds: An Experimental Study in Rabbits

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