Three-dimensional spatiotemporal tracking of nano-objects diffusing in water-filled optofluidic microstructured fiber

Jiang, Shende; Förster, Ronny; Plidschun, Malte; Kobelke, Jens; Ando, Ron Fatobene; Schmidt, Markus A.

doi:10.1515/nanoph-2020-0330

Cited by 8 publications

(13 citation statements)

References 29 publications

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“…Most recently, we have demonstrated particle separation and counting in an uninterrupted flow through a fiber device 31 . Furthermore, structured hollow core fibers allow for the guiding of light directly in the fluid media, which has been used for Raman sensing 32 and for the tracking of diffusion of nanoscale objects 33 , 34 including virus particles 35 . Structured optical waveguides, such as light cages, have been developed for absorption spectroscopy with possible applications in bioanalytics 36 .…”

Section: Introductionmentioning

confidence: 99%

A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection

Parker

Sengupta

Harish

et al. 2022

Sci Rep

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Microfluidics has emerged rapidly over the past 20 years and has been investigated for a variety of applications from life sciences to environmental monitoring. Although continuous-flow microfluidics is ubiquitous, segmented-flow or droplet microfluidics offers several attractive features. Droplets can be independently manipulated and analyzed with very high throughput. Typically, microfluidics is carried out within planar networks of microchannels, namely, microfluidic chips. We propose that fibers offer an interesting alternative format with key advantages for enhanced optical coupling. Herein, we demonstrate the generation of monodisperse droplets within a uniaxial optofluidic Lab-in-a-Fiber scheme. We combine droplet microfluidics with laser-induced fluorescence (LIF) detection achieved through the development of an optical side-coupling fiber, which we term a periscope fiber. This arrangement provides stable and compact alignment. Laser-induced fluorescence offers high sensitivity and low detection limits with a rapid response time making it an attractive detection method for in situ real-time measurements. We use the well-established fluorophore, fluorescein, to characterize the Lab-in-a-Fiber device and determine the generation of $$\sim$$ ∼ 0.9 nL droplets. We present characterization data of a range of fluorescein concentrations, establishing a limit of detection (LOD) of 10 nM fluorescein. Finally, we show that the device operates within a realistic and relevant fluorescence regime by detecting reverse-transcription loop-mediated isothermal amplification (RT-LAMP) products in the context of COVID-19 diagnostics. The device represents a step towards the development of a point-of-care droplet digital RT-LAMP platform.

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Section: Introductionmentioning

confidence: 99%

A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection

Parker

Sengupta

Harish

et al. 2022

Sci Rep

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Section: Resultsmentioning

confidence: 99%

“…Here, it is important to note that the transverse confinement provided by the nanofluidic channel leads to a confined diffusion [25,26] through an increased viscosity, also hindering the diffusion along the longitudinal direction because of the liquid's high bulk modulus. This effect is included here by the so-called average resistance factor R avg , leading to the corrected diffusion constant D c ¼ D f =R avg [14,27] (more details on the resistance factor can be found in the Supporting Information and in the study by Jiang [14] ). The error of the fitted slope of the MSD (Equation ( 1)) defines the precision of the retrieved diffusion D f and hydrodynamic diameter d h and depends on the number of frames N c .…”

Section: Msd Analysismentioning

confidence: 99%

“…[10] A novel NTA scheme that was introduced by the authors in 2015 relies on microstructured optical fibers that include nano-and microfluidic channels along the entire fiber length, supporting fast, label-free, and long-duration observation of nano-objects. [13] This waveguide-based scheme has opened up the field of fiber-based NTA (fNTA), examples of research including the determination of the full 3D trajectory of diffusing gold nanospheres via evanescent-field scattering position retrieval, [14] the simultaneous evaluation of hundreds of diffusing 40 nm gold particles inside antiresonant hollow core fibers, [15] or the measurement of single-virus diffusion (diameter 19 nm) using nanobore optical fibers (NBFs). [13,16] The essential advantage of the fNTA approach within the context of acquiring trajectory with a large number of frames is related to the confinement of the object to the nanofluidic channel, that is, the liquid channel region.…”

Section: Introductionmentioning

confidence: 99%

“…For our experiments, the offset obtained from fitting is À8.11 .110 À16 m 2 (entire dataset and N lag ¼ 2), which is in agreement with the theoretical prediction (À2D f t e /3 ¼ À7.96 Â 10 À16 m 2 ). Note that the nanosphere is actually never lost, that is, is present in all frames of the representative dataset, allowing to straightforwardly apply the MSD analysis reported in the study by Jiang et al[14] (more details can be found in the Supporting Information).To confirm the merits of having a trajectory with an extremely large number of frames, a segmented MSD analysis of the measured diffusion coefficient has been conducted on the representative trajectory (Figure1d). Specifically, the entire trajectory is divided into N s segments of equal length N f ¼ N t =N s which are analyzed separately.…”

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confidence: 97%

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Ultralong Tracking of Fast‐Diffusing Nano‐Objects inside Nanofluidic Channel−Enhanced Microstructured Optical Fiber

Gui

Jiang

Förster

et al. 2021

Advanced Photonics Research

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Nanoparticle tracking analysis (NTA) represents one essential technology to characterize diffusing nanoscale objects. Herein, uncovering dynamic processes and high‐precision measurements requires tracks with thousands of frames to reach high statistical significance, ideally at high frame rates. Optical fibers with nanochannels are used for NTA, successfully demonstrating acquisition of trajectories of fast diffusion nano‐objects with 100 000 frames. Due to the spatial limitation of the central nanofluidic channel, diffusion of objects illuminated by the core mode is confined, enabling the recording of Brownian motion over extraordinarily long time scales at high frame rates. The resulting benefits are discussed on a representative track of a gold nanosphere diffusing in water in over nearly 100 000 frames at 2 kHz frame rate. In addition to the verification of the fiber‐based NTA using two data processing methods, a segmented analysis reveals a correlation between precision of determined diameter and continuous time interval (i.e., number of frames per subtrajectory). The presented results demonstrate the capabilities of fiber‐based NTA in terms of 1) determining diameters with extraordinary high precision of single species and 2) monitoring dynamic processes of the object or the fluidic environment, both of which are relevant within biology, microrheology, and nano‐object characterization.

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Nanoparticle Tracking in Single‐Antiresonant‐Element Fiber for High‐Precision Size Distribution Analysis of Mono‐ and Polydisperse Samples

Nissen

Förster

Wieduwilt

et al. 2022

Small

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Accurate determination of the size distribution of nanoparticle ensembles remains a challenge in nanotechnology‐related applications due to the limitations of established methods. Here, a microstructured fiber‐assisted nanoparticle tracking analysis (FaNTA) realization is introduced that breaks existing limitations through the recording of exceptionally long trajectories of rapidly diffusing polydisperse nanoparticles, resulting in excellent sizing precision and unprecedented separation capabilities of bimodal nanoparticle mixtures. An effective‐single‐mode antiresonant‐element fiber allows to efficiently confine nanoparticles in a light‐guiding microchannel and individually track them over more than 1000 frames, while aberration‐free imaging is experimentally confirmed by cross‐correlation analysis. Unique features of the approach are (i) the highly precise determination of the size distribution of monodisperse nanoparticle ensembles (only 7% coefficient of variation) and (ii) the accurate characterization of individual components in a bimodal mixture with very close mean diameters, both experimentally demonstrated for polymer nanospheres. The outstanding performance of the FaNTA realization can be quantified by introducing a new model for the bimodal separation index. Since FaNTA is applicable to all types of nano‐objects down to sub‐20 nm diameters, the method will improve the precision standard of mono‐ and polydisperse nanoparticle samples such as nano‐plastics or extracellular vesicles.

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Three-dimensional spatiotemporal tracking of nano-objects diffusing in water-filled optofluidic microstructured fiber

Cited by 8 publications

References 29 publications

A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection

A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection

Ultralong Tracking of Fast‐Diffusing Nano‐Objects inside Nanofluidic Channel−Enhanced Microstructured Optical Fiber

Nanoparticle Tracking in Single‐Antiresonant‐Element Fiber for High‐Precision Size Distribution Analysis of Mono‐ and Polydisperse Samples

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