Three-dimensionally porous graphene: A high-performance adsorbent for removal of albumin-bonded bilirubin

Ma, Chun−Fang; Gao, Qiang; Xia, Kaisheng; Huang, Zhiyuan; Han, Bo; Zhou, Chenggang

doi:10.1016/j.colsurfb.2016.10.015

Cited by 57 publications

(33 citation statements)

References 54 publications

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“…The expanded graphite was reported to have substantial adsorption capacity for bovine serum albumin (BSA) from aqueous solution with a view of assessing its application as a wound dressing, in particular for wound drainage [20]. Three-dimensional (3D) porous graphene showed high adsorption capacity towards BSA-bound bilirubin, however the adsorption of BSA was not studied separately and it is not therefore clear whether high adsorption capacity towards bilirubin was a result of a competition between the graphene surface and BSA for bilirubin, or graphene adsorbed the whole bilirubin-BSA complex [21]. Conformational changes of BSA and fibrinogen adsorbed on graphene and graphene oxide nanoribbons were also studied [22].…”

Section: Introductionmentioning

confidence: 99%

Adsorption of Bovine Serum Albumin on Carbon-Based Materials

Seredych

Mikhalovska

Mikhalovsky

et al. 2018

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Abstract:The protein adsorption plays a very important role in biotechnology, biomolecular engineering and it is one of the main factors determining bio-and hemocompatibility of biomedical materials in medical applications, such as blood purification and wound healing. Here we report adsorption properties of two carbon-based materials, thermally expanded graphite (EGr) and graphene nanoplatelets (GnP), for bovine serum albumin (BSA), the most abundant blood plasma protein. The influence of the surface chemistry of expanded graphite on the mechanism of BSA adsorption was studied by using EGr modified with oxygen or nitrogen functionalities. Having low microporosity and the specific surface area in the range of 5 to 50 m 2 /g, the expanded graphite exhibits high protein adsorption capacity at high equilibrium concentrations, which makes this material a potential candidate for biomedical applications as a carrier for high molecular weight (HMW) drug delivery or adsorption of HMW metabolites. At low equilibrium concentrations, the effect of specific protein-surface functional groups interaction reveals the differences between the adsorption affinity of different surface modified EGr materials to BSA. The adsorption of BSA on GnP with a specific surface area of 286 m 2 /g and a developed micro-/mesoporous structure did not follow the same mechanism as seen with EGr materials. At low equilibrium concentration of BSA, GnP exhibits high adsorption efficiency. An important finding is that no release of nanoparticles from expanded graphite adsorbents was observed, which makes them potentially suitable for direct contact with blood and other tissues while very small nanoparticles were noticed in the case of graphene nanoplatelets.

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Section: Introductionmentioning

confidence: 99%

Adsorption of Bovine Serum Albumin on Carbon-Based Materials

Seredych

Mikhalovska

Mikhalovsky

et al. 2018

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“…Moreover, adsorption mainly occurs at the entrances of pores, and as a result, the adsorbed bilirubin may block the pores and reduce its further adsorption. [36] We also employed Langmuir and…”

Section: Bilirubin Adsorptionmentioning

confidence: 99%

Biocompatible Composite Microspheres of Chitin/Ordered Mesoporous Carbon CMK3 for Bilirubin Adsorption and Cell Microcarrier Culture

Yue

Chen

et al. 2022

Macromolecular Bioscience

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Extra bilirubin in the blood can provoke serious illness in patients with severe liver disease. Hemoperfusion is an effective method to remove the extra bilirubin, but its application is limited by the low adsorption efficiency and poor biocompatibility of available adsorbent materials. In this study, chitin/ordered mesoporous carbon CMK3 (Ch/CMK3) microspheres are successfully prepared. Results of characterization experiments indicated that these composite microspheres possess a multilayered porous nanofibrous structure with an extremely large specific surface area (300.19 m 2 g −1 ) and large pore size. Notably, the Ch/CMK3 microspheres demonstrated a high bilirubin adsorption capacity (228.19 mg g −1 ) in phosphate buffer solution (PBS), and an outstanding bilirubin removal ratio (76.78% ± 4.40%) in the plasma of rabbits with hyperbilirubinemia without affecting the protein components. More importantly, the Ch/CMK3 microspheres showed no effect on other blood components, no cytotoxicity, and no systemic toxicity to mice. Cell co-culture experiments revealed that the microspheres can provide a 3-dimensional (3D) space to promote cell adhesion, proliferation, and nutrient exchange. These Ch/CMK3 microspheres featuring a strong ability for bilirubin adsorption and good biocompatibility can be a promising candidate in biomedical applications such as hemoperfusion, cell microcarrier, and 3D tissue engineering.

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“…Recently, Chen et al [38] showed that rGO possessing quinone groups can also assist in electron transfer and enhance the enzyme stability and activity. At the same time, more porous and well-developed external surface of rGO-10min electrode may further promote the proper orientation of BOD that helps to achieve efficient DET between enzyme and rGO [41][42][43]. Therefore, a combination of high amount of carbonyl/quinone groups along with enhanced porosity is a key to improving the electrocatalytic performance of BOD-modified rGO electrodes.…”

Section: Effect Of Treatment Time On Degree Of Surface Functionalizationmentioning

confidence: 99%