Three isolates of novel polyphosphate-accumulating gram-positive cocci, obtained from activated sludge, belong to a new genus, Tetrasphaera gen. nov., and description of two new species, Tetrasphaera japonica sp. nov. and Tetrasphaera australiensis sp. nov.

Maszenan, A. M.; Seviour, Robert J.; Patel, Bharat K. C.; Schümann, Peter; Burghardt, Jutta; Tokiwa, Yutaka; Stratton, Helen

doi:10.1099/00207713-50-2-593

Cited by 149 publications

(131 citation statements)

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“…The morphology of these isolates varies, with T. australiensis and T. japonica always growing as cocci in tetrads or clusters (Maszenan et al, 2000), T. elongata as rod or oval unicells (Hanada et al, 2002), while the morphology of T. jenkinsii depends on the medium on which it is cultured; being filamentous on GS media and growing as irregularly sized cocci in clusters on R2A media (McKenzie et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

“…Members of the Actinobacterial genus Tetrasphaera were suggested to be putative PAOs (Maszenan et al, 2000;Kong et al, 2005), but with a markedly different physiology to Accumulibacter. Both could take up phosphate aerobically and store it intracellularly as polyphosphate, and assimilate a range of substrates under anaerobic conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, the genus Tetrasphaera consists of six species cultured from activated sludge; T. australiensis (strains Ben 109 and Ben 110), T. japonica (strain T1-X7) (Maszenan et al, 2000), T. elongata (strain Lp2) (Hanada et al, 2002), T. elongata (strain ASP12) (Onda and Takii, 2002), T. jenkinsii, T. veronensis and T. vanveenii (McKenzie et al, 2006), but no genome sequence data exist for any of these isolates. As these organisms seem to have an important role in phosphorus removal in EBPR plants (Kong et al, 2005;Nguyen et al, 2011), a metabolic model for Tetrasphaera is needed to clarify what their function in these systems is, and how their ecophysiology might differ from that of Accumulibacter.…”

Section: Introductionmentioning

confidence: 99%

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A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

Kristiansen

Nguyen²,

Saunders³

et al. 2012

The ISME Journal

206

154

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Members of the genus Tetrasphaera are considered to be putative polyphosphate accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) from wastewater. Although abundant in Danish full-scale wastewater EBPR plants, how similar their ecophysiology is to 'Candidatus Accumulibacter phosphatis' is unclear, although they may occupy different ecological niches in EBPR communities. The genomes of four Tetrasphaera isolates (T. australiensis, T. japonica, T. elongata and T. jenkinsii) were sequenced and annotated, and the data used to construct metabolic models. These models incorporate central aspects of carbon and phosphorus metabolism critical to understanding their behavior under the alternating anaerobic/aerobic conditions encountered in EBPR systems. Key features of these metabolic pathways were investigated in pure cultures, although poor growth limited their analyses to T. japonica and T. elongata. Based on the models, we propose that under anaerobic conditions the Tetrasphaerarelated PAOs take up glucose and ferment this to succinate and other components. They also synthesize glycogen as a storage polymer, using energy generated from the degradation of stored polyphosphate and substrate fermentation. During the aerobic phase, the stored glycogen is catabolized to provide energy for growth and to replenish the intracellular polyphosphate reserves needed for subsequent anaerobic metabolism. They are also able to denitrify. This physiology is markedly different to that displayed by 'Candidatus Accumulibacter phosphatis', and reveals Tetrasphaera populations to be unusual and physiologically versatile PAOs carrying out denitrification, fermentation and polyphosphate accumulation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

Kristiansen

Nguyen²,

Saunders³

et al. 2012

The ISME Journal

206

154

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“…(2) Members of the genus Tetrasphaera grow very slowly and utilize only a limited number of substrates (Maszenan et al, 2000;Ishikawa & Yokota, 2006). (3) Tetrasphaera duodecadis IAM 14868 T indispensably requires vitamin B 12 for robust growth (Lochhead, 1958;Ishikawa & Yokota, 2006) and isoprenoid quinones cannot be detected in Tetrasphaera veronensis Ver1 T (McKenzie et al 2006).…”

mentioning

confidence: 99%

Phycicoccus endophyticus sp. nov., an endophytic actinobacterium isolated from Bruguiera gymnorhiza

Liu

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A novel endophytic actinobacterium, designated strain IP6SC6 T , was isolated from surfacesterilized bark of Bruguiera gymnorhiza collected from Zhanjiang Mangrove Forest National Nature Reserve in Guangdong, China. Cells of strain IP6SC6 T were Gram-stain-positive, aerobic, non-spore-forming, non-motile rods. Strain IP6SC6 T grew at 20-42 8C (optimum, 37 8C), at pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-8 % (w/v) NaCl (optimum, 0-2 %). Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan, galactose and glucose as whole-cell sugars, and MK-8(H 4 ) as the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and an unknown lipid. The major fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 . The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain IP6SC6 T belonged to the genus Phycicoccus and shared the highest sequence similarity with Phycicoccus jejuensis NRRL B-24460 T (96.97 %). On the basis of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain IP6SC6 T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus endophyticus sp. nov. is proposed. The type strain is IP6SC6 T (5DSM 100020 T 5CGMCC 4.7300 T ).

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“…Furthermore, the genus Bogoriella (Groth et al, 1997) and the genus Rarobacter (Yamamoto et al, 1988) were excluded for obligate alkaliphily and for requirement of catalase, haemoglobin or haemin for growth in air, respectively. The genera Tetrasphaera (Maszenan et al, 2000) and Mycobacterium Lehmann and Neumann 1896 are not represented due to their extremely slowly growing species.…”

Section: Composition Of the Identification Databasementioning

confidence: 99%

Identification of coryneform bacteria and related taxa by Fourier-transform infrared (FT-IR) spectroscopy.

Oberreuter¹,

Seiler²,

Scherer³

2002

International Journal of Systematic and Evolutionary Microbiology

128

112

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Three isolates of novel polyphosphate-accumulating gram-positive cocci, obtained from activated sludge, belong to a new genus, Tetrasphaera gen. nov., and description of two new species, Tetrasphaera japonica sp. nov. and Tetrasphaera australiensis sp. nov.

Cited by 149 publications

References 57 publications

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

Phycicoccus endophyticus sp. nov., an endophytic actinobacterium isolated from Bruguiera gymnorhiza

Identification of coryneform bacteria and related taxa by Fourier-transform infrared (FT-IR) spectroscopy.

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