Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae

Ohkita, Shuhei; Lee, Yui; Nguyen, Quyet; Ikeda, Kenichi; Suzuki, Nobuhiro; Nakayashiki, Hitoshi

doi:10.1016/j.virol.2019.05.015

Cited by 24 publications

(17 citation statements)

References 40 publications

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“…Members of the genus Ourmiavirus typically contain three genomic RNA segments, with a unique bacilliform virion structure (Brown, 1997;Hull, 2014). Recently, several mycoviruses that are phylogenetically related to ourmiaviruses have been identified (Donaire et al, 2016;Hrabakova et al, 2017;Illana et al, 2017;Li et al, 2019;Ohkita et al, 2019). Therefore, ICTV has created a new family (Botourmiaviridae) to adapt these ourmia-like viruses (classified into three genera) and existing ourmiaviruses.…”

Section: Introductionmentioning

confidence: 99%

A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi

Wang

Xiè

et al. 2020

Front. Microbiol.

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Recently, an increasing number of ourmia-like viruses have been found in fungi; however, the features of these viruses remain unknown. Here, we report a novel ourmia-like virus isolated from Sclerotinia sclerotiorum. This virus, named S. sclerotiorum ourmia-like virus 4 (SsOLV4), has a genome 2,982 nt in length with a G-pentamer (GGGGG) at the 5terminus and a C-pentamer (CCCCC) at the 3-terminus. The SsOLV4 genome has only one large putative open reading frame (ORF) predicted with both standard codes and mitochondrial codes and encodes an RNA-dependent RNA polymerase (RdRp). SsOLV4 is closely phylogenetically related to Pyricularia oryzae ourmia-like virus 1, with 42% identity between the RdRp amino acid sequences. We constructed full-length cDNA of SsOLV4 and synthesized RNA in vitro using the T7 RNA polymerase. The synthesized RNA could transfect S. sclerotiorum protoplasts efficiently. We further found that viral RNA could infect mycelia when mixed with PEG buffer. Our study suggests that a novel genus in family Botourmiaviridae should be established for SsOLV4 and other related viruses and demonstrates that one single-stranded RNA segment encoding RdRp is sufficient for ourmia-like viruses in fungi.

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Section: Introductionmentioning

confidence: 99%

A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi

Wang

Xiè

et al. 2020

Front. Microbiol.

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“…Similar to Narnaviriridae and Mitoviridae, the Botourmiaviridae family is composed of viruses infecting the cytoplasm of plant cells and, unlike these two families, their genome is composed of three monocistronic segments [45]. Nevertheless, some studies have identified members of the Ourmiaviridae family infecting the cytoplasm of filamentous fungi [46].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Mitovirus of the Sand Fly Lutzomyia longipalpis Using Genomic and Virus–Host Interaction Signatures

Fonseca

Ferreira

Silva

et al. 2020

Viruses

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Hematophagous insects act as the major reservoirs of infectious agents due to their intimate contact with a large variety of vertebrate hosts. Lutzomyia longipalpis is the main vector of Leishmania chagasi in the New World, but its role as a host of viruses is poorly understood. In this work, Lu. longipalpis RNA libraries were subjected to progressive assembly using viral profile HMMs as seeds. A sequence phylogenetically related to fungal viruses of the genus Mitovirus was identified and this novel virus was named Lul-MV-1. The 2697-base genome presents a single gene coding for an RNA-directed RNA polymerase with an organellar genetic code. To determine the possible host of Lul-MV-1, we analyzed the molecular characteristics of the viral genome. Dinucleotide composition and codon usage showed profiles similar to mitochondrial DNA of invertebrate hosts. Also, the virus-derived small RNA profile was consistent with the activation of the siRNA pathway, with size distribution and 5′ base enrichment analogous to those observed in viruses of sand flies, reinforcing Lu. longipalpis as a putative host. Finally, RT-PCR of different insect pools and sequences of public Lu. longipalpis RNA libraries confirmed the high prevalence of Lul-MV-1. This is the first report of a mitovirus infecting an insect host.

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“…There are examples of mycoviruses related to families of viruses that infect other hosts. Among ssRNA1 mycoviruses, since the discovery of the first ourmia-like mycoviruses (3,5,15) related to plant ourmiaviruses (16), several similar ourmia-like mycoviruses have been discovered (17)(18)(19); also, some of the identified ssRNA-mycoviruses are related to plant or animal viruses (4,11,20). However, a clear example of host change is the finding of the plant cucumber mosaic virus inside the plant-pathogenic fungus Rhizoctonia solani (6).…”

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confidence: 99%

Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus

Ruiz-Padilla

Rodríguez‐Romero

Gómez-Cid

et al. 2021

mBio

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Botrytis cinerea is one of the most important plant-pathogenic fungus. Products based on microorganisms can be used in biocontrol strategies alternative to chemical control, and mycoviruses have been explored as putative biological agents in such approaches. Here, we have explored the mycovirome of B. cinerea isolates from grapevine of Italy and Spain to increase the knowledge about mycoviral diversity and evolution, and to search for new widely distributed mycoviruses that could be active ingredients in biological products to control this hazardous fungus. A total of 248 B. cinerea field isolates were used for our metatranscriptomic study. Ninety-two mycoviruses were identified: 62 new mycoviral species constituting putative novel viral genera and families. Of these mycoviruses, 57 had a positive-sense single-stranded RNA (ssRNA) genome, 19 contained a double-stranded RNA (dsRNA) genome, 15 had a negative-sense ssRNA genome, and 1 contained a single-stranded DNA (ssDNA) genome. In general, ssRNA mycoviruses were widely distributed in all sampled regions, the ssDNA mycovirus was more frequently found in Spain, and dsRNA mycoviruses were scattered in some pools of both countries. Some of the identified mycoviruses belong to clades that have never been found associated with Botrytis species: Botrytis-infecting narnaviruses; alpha-like, umbra-like, and tymo-like ssRNA+ mycoviruses; trisegmented ssRNA− mycovirus; bisegmented and tetrasegmented dsRNA mycoviruses; and finally, an ssDNA mycovirus. Among the results obtained in this massive mycovirus screening, the discovery of novel bisegmented viruses, phylogenetically related to narnaviruses, is remarkable. IMPORTANCE The results obtained here have expanded our knowledge of mycoviral diversity, horizontal transfers, and putative cross-kingdom events. To date, this study presents the most extensive and wide diversity collection of mycoviruses infecting the necrotrophic fungus B. cinerea. The collection included all types of mycoviruses, with dsRNA, ssRNA+, ssRNA–, and ssDNA genomes, most of which were discovered here, and some of which were previously reported as infecting B. cinerea or other plant-pathogenic fungi. Some of these mycoviruses are reported for the first time here associated with B. cinerea, as a trisegmented ssRNA– mycovirus and as an ssDNA mycovirus, but even more remarkablly, we also describe here four novel bisegmented viruses (binarnaviruses) not previously described in nature. The present findings significantly contribute to general knowledge in virology and more particularly in the field of mycovirology.

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Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae

Cited by 24 publications

References 40 publications

A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi

A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi

Characterization of a Novel Mitovirus of the Sand Fly Lutzomyia longipalpis Using Genomic and Virus–Host Interaction Signatures

Novel Mycoviruses Discovered in the Mycovirome of a Necrotrophic Fungus

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