Three transcripts of EDS1-like genes respond differently to Vitis flexuosa infection

Abstract

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Enhanced disease susceptibility1 (EDS1) is a regulator of basal defense responses required for resistance mediated by TIR-NBS-LRR containing R proteins. We identified three transcripts of EDS1-like genes encompassing diverse/ separate expression patterns, based on the transcriptome analysis by Next Generation Sequencing (NGS) of V. flexuosa inoculated with Elsinoe ampelina. These genes were designated VfEDL1 (Vitis flexuosa Enhanced Disease Susceptibility1-like1), VfEDL2 and VfEDL3, and contained 2464, 1719 and 1599 bp, with 1791, 1227 and 1599 bp open reading frames (ORFs), encoding proteins of 596, 408 and 532 amino acids, respectively. The predicted amino acid sequences of all three genes showed the L-family lipase-like domain (class 3 lipase domain), and exhibited a potential lipase catalytic triad, aspartic acid, histidine and serine in the conserved G-X-S-X-G. All three VfEDL genes were upregulated at 1 hpi against the bacterial and fungal pathogens Rizhobium vitis and E. ampelina, respectively, except VfEDL1, which was downregulated against E. ampelina at all time points. Against E. ampelina, VfEDL2 and VfEDL3 showed downregulated expression at later time points. When evaluated against R. vitis, VfEDL1 showed downregulated expression at all time points after 1 hpi, while VfEDL3 showed upregulation up to 24 hpi. Based on the expression response, all three genes may be involved in plant resistant responses against R. vitis, and VfEDL2 and VfEDL3 show additional resistant responses against E. ampelina infection.

Comparative transcriptome analysis of leaves from two grapevine species reveals an early gene expression profile associated with Elsinoe ampelina resistance

“…In contrast, SA signaling is typically involved in plant resistance to biotrophic pathogens [53], with the positive SA regulator EDS1 conferring basal defense against various pathogens by interacting with a subset of plant resistance (R) genes [54]. Three EDS1-like genes with distinct expression patterns were identified in V. flexuosa inoculated with E. ampelina, and two of them(VfEDL2 and VfEDL3)are known to be induced by infection [55]. In our study, up-regulation of EDS1 at the early time point in the HR grape is indicative of an SA pathway response in the resistant grape.…”

Current status and future prospects of grapevine anthracnose caused by Elsinoe ampelina: An important disease in humid grape‐growing regions

“…Jayasankar et al (2003) have reported that the spore germination and the hyphal growth of E. ampelina are significantly inhibited by a V. vinifera thaumatin-like protein. Several genes encoding β-1,3glucanase , nucleotide-binding site domain proteins (Islam et al, 2015a;Seehalak et al, 2011), receptor-like protein kinase (Islam et al, 2015b), glutathione S-transferase (Ahn et al, 2016), Mlo-like proteins (Islam & Yun, 2016a), carboxylesterase proteins (Islam & Yun, 2016b), and EDS1-like proteins (Islam & Yun, 2017) are responsive genes to E. ampelina infection, indicating that these genes may participate in the disease-resistance response.…”

Emergent Ascomycetes in Viticulture: An Interdisciplinary Overview

“…Ahn et al (2014a) de novo assembled the transcriptome of the resistant Korean species V. flexuosa inoculated with the pathogen. This analysis led to the identification and subsequent characterization of many resistance related responsive genes to E. ampelina inoculation, such as β-1,3-glucanase genes (Ahn et al, 2014b), RPS5-like genes (Islam et al, 2015a), receptor-like protein kinase (RLK) genes (Islam et al, 2015b), Mlo-like genes (Islam and Yun, 2016a), carboxylesterase genes (Islam and Yun, 2016b), glutathione S-transferase genes (Ahn et al, 2016) and EDS1-like genes (Islam and Yun, 2017). Several V. vinifera thaumatin-like proteins ( VvTLP ) genes responsive to anthracnose were also recently identified (Yan et al, 2017).…”