Thyrotropin-Releasing Hormone

Investigation of herpes simplex virus type 1 genes encoding multiply inserted membrane proteins

“…Synthetic oligopeptides representing three different Immunoprecipitation of the UL20 gene product. Antiserum was raised against a synthetic oligopeptide, NH2-Gln-Met-Leu-Pro-Pro-Thr-Asp-Pro-Leu-Arg-ThrArg-Tyr-COOH, representing amino acids 168 to 179 of the UL20 ORF with a carboxy-terminal tyrosine residue added to facilitate coupling to BSA (Bassiri et al, 1979). Proteins from HSV-l-infected cell extracts labelled with [35S]methionine (lane 1) were precipitated using immune serum in the presence of 10 ~tg of a control peptide (lane 2) or the peptide against which the serum was raised (lane 3).…”

Identification of Two Genes in the Unique Short Region of Pseudorabies Virus; Comparison with Herpes Simplex Virus and Varicella-zoster Virus

“…Two peptides, corresponding to amino acid residues RRPSADEILNFG of the predicted protein kinase and amino acid residues RRAADEDTPRQ of the predicted 28K protein, respectively, were synthesized on a Biosearch peptide synthesizer and coupled to bovine serum albumin as described (Bassiri et al, 1979). Adult NZ rabbits were immunized by injection of 3 mg of conjugate in complete Freund's adjuvant both in the hind leg lymph nodes and intradermally at different sites in the back.…”

Identification of the Herpes Simplex Virus Protein Kinase as the Product of Viral Gene US3

“…The peptide NHz-Tyr-Cys-Leu-Pro-Leu-Phe-Gln-Gln-Lys-COOH (Cambridge Research Biochemicals, Cambridge, U.K.) corresponds to the C-terminal eight amino acids of the predicted US3 protein sequence plus an additional tyrosine residue which facilitated coupling of the peptide to bovine serum albumin (BSA) (Bassiri et al,, 1979). Rabbits were immunized with the peptide-BSA conjugate as described previously and anti-BSA antibodies were removed from the immune sera by incubation with BSA-Sepharose.…”