Tick-borne encephalitis virus isolates from natural foci of the Irkutsk region: clarification of the genotype landscape

Мельникова, О. В.; Adelshin, R. V.; Корзун, В. М.; Trushina, Yu. N.; Андаев, Е. И.

doi:10.18821/0507-4088-2016-61-5-229-234

Cited by 3 publications

(2 citation statements)

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“…Многие исследователи при проведении диагностики КЭ зачастую сталкиваются с несовпадением положительных результатов в ИФА и ПЦР [22,31,33]. Кроме того, выявленные положительные пробы в клещах и лейкоцитарной фракции крови людей в ИФА или в ПЦР довольно часто не подтверждаются изоляцией ВКЭ.…”

Section: основная частьunclassified

“…Мельниковой и соавт. [33], большая часть суспензий, содержащих антиген ВКЭ, не вызывала клинических проявлений болезни при заражении ими белых мышей. Авторам удалось выделить вирус из клещевых суспензий в 25% наблюдений, из мозговых суспензий мелких млекопитающих -в 10,5%.…”

Section: основная частьunclassified

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Tick-borne encephalitis in the Far East focal region of the Eurasian continent

Леонова¹

2020

Journal of microbiology, epidemiology and immunobiology

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The analysis of the activity of natural foci of tick-borne encephalitis (TBE) in the Far Eastern Federal District (Primorsky and Khabarovsk Territories, the Jewish Autonomous and Amur Regions), represented by a single Sikhote-Alin focal region was performed. Endemic territories of China, Korea, Japan are adjacent to this focal region. In the last decade the decrease of the infection rate in ixodid ticks and the morbidity of TBE compared to the 1990s was observed. Data on the infection rates in ixodid ticks based on ELISA, PCR and TBEV isolation are often discrepant. Active circulation of TBEV that involved dominant and less significant species of ixodid ticks and mouse-like rodents has been demonstrated since 1952 in China, since the 1990s in Japan and since 2011 in South Korea. The circulation of TBEV strains of the Far Eastern subtype was determined in all these territories. The exception was 7 strains isolated in South Korea, which appeared to be the European subtype based on the results of molecular genetic analysis. Two new strains of TBEV from the organs of wild rodents Marmota himalayana were isolated in 2017 in the territories of previously unknown natural foci in the Tien Shan, China. The genetic divergence of these strains from three TBEV subtypes allowed us to establish the existance of a new Himalayan TBEV subtype (Him-TBEV), which, according to the authors, has been "hidden" for hundreds of years. One can also speculate the processes of emergence of new TBEV subtypes The most complete picture of the Far Eastern population of TBEV was obtained in the last decade of the twentieth century, which served as the basis and impetus for the study and comparative analysis of the differences in numerous strains of TBEV isolated on the territory of the Eurasian continent.

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Section: основная частьunclassified

Tick-borne encephalitis in the Far East focal region of the Eurasian continent

Леонова¹

2020

Journal of microbiology, epidemiology and immunobiology

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Tickborne Encephalitis Virus

Beltz¹

2021

Zika and Other Neglected and Emerging Flaviviruses

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Comparative Analysis of Methods Efficiency of Virification of the Tick-Borne Encephalite Virus

Леонова¹

2019

RCLD

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Based on experimental studies with 10-fold dilutions of two the strains TBEV of the Far Eastern subtype, different in molecular-genetic characteristic complex data of simultaneously taking into account three indicators of their verification (virus titer, ELISA and PCR-RT) were obtained. The efficiency of detecting a genetic marker in PCR compared with ELISA for a weakly pathogenic strain with defects in the genetic structure was higher by a factor of 10, and for a highly pathogenic strain by a factor of 5,000. At the same time, positive results in both reactions with respect to two strains were detected with a virus titer of not less than 1-1.5 log TCID50, i.e. this level of virus in the sample is defined as epidemically significant. An algorithm for conducting research on the verification of TBEV is proposed: 1) Ticks collected from vegetation can be examined by ELISA or by PCR. All positive results can be summarized and considered viral ticks; 2) All samples with positive results only in PCR or ELISA must be investigated in two reactions in order to obtain confirmation of the possible infectivity of the pathogen; 3) To obtain a fast complex result of infection of the removed ticks from patients or blood after a tick bite, studies should be carried out simultaneously in two reactions simultaneously in ELISA and PCR; 4) Isolation virus should be carried out in biological samples (ticks collected from vegetation, ticks removed from patients, the blood of patients with suspected TBE, mammals) only with the same results in PCR and ELISA. Thus, such an approach to verifying TBEV in a tick or in the blood of patients will improve the reliability of laboratory diagnostics, identifying not only markers of TBEV, but also determining the infectivity of the pathogen, which may be the basis for the appointment of early intensive antiviral therapy.

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Tick-borne encephalitis virus isolates from natural foci of the Irkutsk region: clarification of the genotype landscape

Cited by 3 publications

References 9 publications

Tick-borne encephalitis in the Far East focal region of the Eurasian continent

Tick-borne encephalitis in the Far East focal region of the Eurasian continent

Tickborne Encephalitis Virus

Comparative Analysis of Methods Efficiency of Virification of the Tick-Borne Encephalite Virus

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