Time for a Nuclear Meeting: Protein Trafficking and Chromatin Dynamics Intersect in the Plant Circadian System

Herrero, Eva; Davis, Seth J

doi:10.1093/mp/sss010

Cited by 36 publications

(34 citation statements)

References 137 publications

(208 reference statements)

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“…Since ELF4 was preferentially nuclear (Figures 3 and 7), our results are consistent with ELF4 acting to increase the nuclear pool of ELF3. Analogous mechanisms of cytoplasmic-tonuclear distribution dynamics are found in several circadian systems (Herrero and Davis, 2012). In plants, PRR5 was reported to stabilize the TOC1 nuclear pool (Wang et al, 2010).…”

Section: Lux Is Downstream Of Elf4mentioning

confidence: 70%

“…Both clock-and light-signaling components localize in nuclear bodies (Má s et al, 2000;Chen, 2008;Yu et al, 2008;Wang et al, 2010;Herrero and Davis, 2012). For ELF3, we found that the C termini were required for nuclear bodies (Figures 2 and 4).…”

Section: Lux Is Downstream Of Elf4mentioning

confidence: 77%

“…Hence, both transcriptional repression mediated cooperatively by ELF3/ELF4 and LUX and transcriptional activation mediated by CCA1 are required for rhythmic expression of PRR9. The competition between activator and repressor complexes has been found to be critical for sustaining circadian oscillations in plants (Li et al, 2011;Herrero and Davis, 2012) and in other clock systems, such as in mice and flies (Matsumoto et al, 2007;Ukai-Tadenuma et al, 2008;Ukai-Tadenuma et al, 2011). Therefore, it is plausible that competition of coactivators and corepressors at the promoters of clock-controlled genes is generally important for circadian oscillator function in eukaryotic organisms.…”

Section: Lux Is Downstream Of Elf4mentioning

confidence: 99%

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EARLY FLOWERING4 Recruitment of EARLY FLOWERING3 in the Nucleus Sustains the Arabidopsis Circadian Clock

et al. 2012

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The plant circadian clock is proposed to be a network of several interconnected feedback loops, and loss of any component leads to changes in oscillator speed. We previously reported that Arabidopsis thaliana EARLY FLOWERING4 (ELF4) is required to sustain this oscillator and that the elf4 mutant is arrhythmic. This phenotype is shared with both elf3 and lux. Here, we show that overexpression of either ELF3 or LUX ARRHYTHMO (LUX) complements the elf4 mutant phenotype. Furthermore, ELF4 causes ELF3 to form foci in the nucleus. We used expression data to direct a mathematical position of ELF3 in the clock network. This revealed direct effects on the morning clock gene PRR9, and we determined association of ELF3 to a conserved region of the PRR9 promoter. A cis-element in this region was suggestive of ELF3 recruitment by the transcription factor LUX, consistent with both ELF3 and LUX acting genetically downstream of ELF4. Taken together, using integrated approaches, we identified ELF4/ELF3 together with LUX to be pivotal for sustenance of plant circadian rhythms.

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Section: Lux Is Downstream Of Elf4mentioning

confidence: 70%

Section: Lux Is Downstream Of Elf4mentioning

confidence: 77%

Section: Lux Is Downstream Of Elf4mentioning

confidence: 99%

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EARLY FLOWERING4 Recruitment of EARLY FLOWERING3 in the Nucleus Sustains the Arabidopsis Circadian Clock

et al. 2012

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“…The core clockwork consists of proteins that generate self-sustained oscillations by feedback on transcription of their own genes. These negative feedback loops are controlled by several different mechanisms, including protein phosphorylation, protein turnover, gene expression, and chromatin remodeling to maintain a 24-h period (Schöning and Staiger, 2005;Más, 2008;Herrero and Davis, 2012).…”

Section: The Circadian Clockmentioning

confidence: 99%

Alternative Splicing at the Intersection of Biological Timing, Development, and Stress Responses

2013

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High-throughput sequencing for transcript profiling in plants has revealed that alternative splicing (AS) affects a much higher proportion of the transcriptome than was previously assumed. AS is involved in most plant processes and is particularly prevalent in plants exposed to environmental stress. The identification of mutations in predicted splicing factors and spliceosomal proteins that affect cell fate, the circadian clock, plant defense, and tolerance/sensitivity to abiotic stress all point to a fundamental role of splicing/AS in plant growth, development, and responses to external cues. Splicing factors affect the AS of multiple downstream target genes, thereby transferring signals to alter gene expression via splicing factor/AS networks. The last two to three years have seen an ever-increasing number of examples of functional AS. At a time when the identification of AS in individual genes and at a global level is exploding, this review aims to bring together such examples to illustrate the extent and importance of AS, which are not always obvious from individual publications. It also aims to ensure that plant scientists are aware that AS is likely to occur in the genes that they study and that dynamic changes in AS and its consequences need to be considered routinely.

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“…The regulation of protein turnover and nucleocytoplasmic distribution is part of the molecular mechanism at the core of the circadian clock (Harmer, 2009;Herrero and Davis, 2012;Más, 2008;Tataroğlu and Schafmeier, 2010). F-box proteins are key components of SCF-type E3 ligases that target proteins for degradation via the 26S proteasome pathway and play a substantial role in all circadian systems (Godinho et al, 2007;He et al, 2003;Ko et al, 2002;Koh et al, 2006;Siepka et al, 2007;Somers et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

The F-box protein ZEITLUPE controls stability and nucleocytoplasmic partitioning of GIGANTEA

et al. 2013

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SUMMARYNucleocytoplasmic partitioning of core clock components is essential for the proper operation of the circadian system. Previous work has shown that the F-box protein ZEITLUPE (ZTL) and clock element GIGANTEA (GI) heterodimerize in the cytosol, thereby stabilizing ZTL. Here, we report that ZTL post-translationally and reciprocally regulates protein levels and nucleocytoplasmic distribution of GI in Arabidopsis. We use ectopic expression of the N-terminus of ZTL, which contains the novel, light-absorbing region of ZTL (the LOV domain), transient expression assays and ztl mutants to establish that the levels of ZTL, a cytosolic protein, help govern the abundance and distribution of GI in the cytosol and nucleus. Ectopic expression of the ZTL N-terminus lengthens period, delays flowering time and alters hypocotyl length. We demonstrate that these phenotypes can be explained by the competitive interference of the LOV domain with endogenous GI-ZTL interactions. A complex of the ZTL N-terminus polypeptide with endogenous GI (LOV-GI) blocks normal GI function, causing degradation of endogenous ZTL and inhibition of other GI-related phenotypes. Increased cytosolic retention of GI by the LOV-GI complex additionally inhibits nuclear roles of GI, thereby lengthening flowering time. Hence, we conclude that under endogenous conditions, GI stabilization and cytoplasmic retention occurs naturally through a LOV domainmediated GI-ZTL interaction, and that ZTL indirectly regulates GI nuclear pools by sequestering GI to the cytosol. As the absence of either GI or ZTL compromises clock function and diminishes the protein abundance of the other, our results highlight how their reciprocal co-stabilization is essential for robust circadian oscillations.

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Time for a Nuclear Meeting: Protein Trafficking and Chromatin Dynamics Intersect in the Plant Circadian System

Cited by 36 publications

References 137 publications

EARLY FLOWERING4 Recruitment of EARLY FLOWERING3 in the Nucleus Sustains the Arabidopsis Circadian Clock

EARLY FLOWERING4 Recruitment of EARLY FLOWERING3 in the Nucleus Sustains the Arabidopsis Circadian Clock

Alternative Splicing at the Intersection of Biological Timing, Development, and Stress Responses

The F-box protein ZEITLUPE controls stability and nucleocytoplasmic partitioning of GIGANTEA

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